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Species: Saccharomyces cerevisiae
Genetic Insert: U6 U57A
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4783; Vector Backbone:pRS314; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33547186
Comments: Please note that this plasmid contains an additional IS4-like element ISVsa5 family transposase compared to the pRS314 backbone.
Proper citation: RRID:Addgene_169927 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: U6 U57C
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4783; Vector Backbone:pRS314; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33547186
Comments: Please note that this plasmid contains an additional IS4-like element ISVsa5 family transposase compared to the pRS314 backbone.
Proper citation: RRID:Addgene_169928 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yeast Tim12
Vector Backbone Description: Vector Backbone:pET28a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33974880
Proper citation: RRID:Addgene_170281 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Mini-Tn6677, GNAc cassette, lacI
Vector Backbone Description: Vector Backbone:pUC57mini; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34152213
Proper citation: RRID:Addgene_170637 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA_RNH1_RNH201
Vector Backbone Description: Vector Backbone:p0054; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34107026
Proper citation: RRID:Addgene_177271 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:6300; Vector Backbone:pRS415U; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34107026
Proper citation: RRID:Addgene_177272 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADH1t:T7p:cgRNA_HIS3_stop:tZ
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pEDJ400; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34107026
Proper citation: RRID:Addgene_177275 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADH1t:HIS3_23∆29-XII-5 :<-PGK1p
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pCfB2909; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34107026
Comments: Integrative plasmid
Please note: Plasmid contains a 68bp insertion in mADH1t compared to the depositor's provided sequence. This insertion is not known to affect plasmid function.
Proper citation: RRID:Addgene_177274 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL80
Vector Backbone Description: Backbone Size:7855; Vector Backbone:pCaSpeR4; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10197526
Proper citation: RRID:Addgene_17748 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pTEFmut8-mCherry
Vector Backbone Description: Vector Backbone:Empty backbone; Vector Types:Yeast Expression, Used as donor DNA in genome integration after linearisation with NotI; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35069506
Proper citation: RRID:Addgene_177706 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
Vector Backbone Description: Vector Backbone:Empty backbone; Vector Types:Yeast Expression, Used as donor DNA in genome integration after linearisation with NotI; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35069506
Proper citation: RRID:Addgene_177711 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter
Vector Backbone Description: Backbone Size:5726; Vector Backbone:pRS426; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27323850
Comments: Please refer to Roney et al. 2016 Sci Rep. "Improvement of the reverse tetracycline transactivator by single amino acid substitutions that reduce leaky target gene expression to undetectable levels" (doi: 10.1038/srep27697) for more information.
Proper citation: RRID:Addgene_177925 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter
Vector Backbone Description: Backbone Size:5726; Vector Backbone:pRS426; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27323850
Comments: Please refer to Roney et al. 2016 Sci Rep. "Improvement of the reverse tetracycline transactivator by single amino acid substitutions that reduce leaky target gene expression to undetectable levels" (doi: 10.1038/srep27697) for more information.
Proper citation: RRID:Addgene_177924 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:12778056
Comments: does not contain lacI/
ERG1, ERG8, MVD1: S. cerevisiae/
idi: E. Coli
Proper citation: RRID:Addgene_17816 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MevT
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBAD24; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17239639
Proper citation: RRID:Addgene_17820 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: I-SceI
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33589718
Proper citation: RRID:Addgene_165041 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MAT Z1
Vector Backbone Description: Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t, Daran Lab; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: Addgene QC identified differences to the theoretical sequence that the depositing lab does not believe are of functional concern.
Proper citation: RRID:Addgene_166099 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL2
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrXII:291472..291491 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166088 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CUP1-1
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrVIII:212613..212632 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166086 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MET16
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrXVI:877523..877542 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome.
Addgene QC identified several discrepancies compared to the theoretical reference.
Proper citation: RRID:Addgene_166091 Copy
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