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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
MSP11-bio
 
Resource Report
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RRID:Addgene_47734 Codon-optimised MSP11 Synthetic Ampicillin PMID:24043421 Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Exogenous signal peptide of mouse origin, changed Serine41, Serine87, Threonine93 and Serine128 to Alanine residues 2026-08-15 01:15:43 0
Pf113-bio
 
Resource Report
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1+ mentions
RRID:Addgene_47729 Codon-optimised Pf113 Synthetic Ampicillin PMID:24043421 Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Exogenous signal peptide of mouse origin, changed Serine209, Serine270, Threonine319, Serine362, Serine663, Threonine699, Serine781, Threonine878 and Threonine940 to Alanine residues 2026-08-15 01:15:44 2
pFAB3869
 
Resource Report
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RRID:Addgene_47842 promoter 11 and BCD1 Synthetic Kanamycin PMID:23474465 http://biofab.org/services RFP fluorescence (Arbitrary Units, AU) is used to measure the efficiency of the promoter/BCD combination. Promoter/BCD combinations ranged from a low score of 6.14 to a high score of 634.54. The score for this combination was: 122.59 Backbone Size:3000; Vector Backbone:pFAB1781; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:15:44 0
pFAB3845
 
Resource Report
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RRID:Addgene_47840 promoter 9 and BCD1 Synthetic Kanamycin PMID:23474465 http://biofab.org/services RFP fluorescence (Arbitrary Units, AU) is used to measure the efficiency of the promoter/BCD combination. Promoter/BCD combinations ranged from a low score of 6.14 to a high score of 634.54. The score for this combination was: 69.62 Backbone Size:3000; Vector Backbone:pFAB1781; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:15:44 0
pFAB3737
 
Resource Report
Resource Website
RRID:Addgene_47838 promoter 7 and BCD1 Synthetic Kanamycin PMID:23474465 http://biofab.org/services RFP fluorescence (Arbitrary Units, AU) is used to measure the efficiency of the promoter/BCD combination. Promoter/BCD combinations ranged from a low score of 6.14 to a high score of 634.54. The score for this combination was: 404.1 Backbone Size:3000; Vector Backbone:pFAB1781; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:15:46 0
CLAG3.2-bio
 
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RRID:Addgene_47797 Codon-optimised CLAG3.2 Synthetic Ampicillin PMID:24043421 Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Exogenous signal peptide of mouse origin, changed Threonine75, Threonine114, Threonine476, Threonine542, Threonine641, Serine941, Serine1013, Serine1062, Threonine1130 and Threonine1373 to Alanine residues 2026-08-15 01:15:44 0
pFAB3999
 
Resource Report
Resource Website
RRID:Addgene_47830 promoter 14 and BCD23 Synthetic Kanamycin PMID:23474465 http://biofab.org/services RFP fluorescence (Arbitrary Units, AU) is used to measure the efficiency of the promoter/BCD combination. Promoter/BCD combinations ranged from a low score of 6.14 to a high score of 634.54. The score for this combination was: 52.63 Backbone Size:3000; Vector Backbone:pFAB1781; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:15:44 0
pFAB3701
 
Resource Report
Resource Website
RRID:Addgene_47835 promoter 4 and BCD1 Synthetic Kanamycin PMID:23474465 http://biofab.org/services RFP fluorescence (Arbitrary Units, AU) is used to measure the efficiency of the promoter/BCD combination. Promoter/BCD combinations ranged from a low score of 6.14 to a high score of 634.54. The score for this combination was: 50.75 Backbone Size:3000; Vector Backbone:pFAB1781; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:15:46 0
PTRAMP-bio
 
Resource Report
Resource Website
RRID:Addgene_47793 Codon-optimised PTRAMP Synthetic Ampicillin PMID:24043421 Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Exogenous signal peptide of mouse origin, changed Serine114, Serine151, Serine157, Serine172, Threonine197, Threonine204 and Serine255 to Alanine residues 2026-08-15 01:15:44 0
RAP1-bio
 
Resource Report
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RRID:Addgene_47794 Codon-optimised RAP1 Synthetic Ampicillin PMID:24043421 Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Exogenous signal peptide of mouse origin, changed Serine74, Threonine84, Serine92, Serine170, Threonine329, Serine357 and Threonine404 to Alanine residues 2026-08-15 01:15:44 0
PF3D7_1431400-bio
 
Resource Report
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RRID:Addgene_47791 Codon-optimised PF3D7_1431400 Synthetic Ampicillin PMID:24043421 Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Exogenous signal peptide of mouse origin, changed Serine27, Serine31, Serine35, Threonine70, Serine102, Serine103, Serine300, Threonine309, Threonine313, Threonine387, Serine481, Serine497, Serine498, Serine502, Serine513, Serine551, Serine574, Threonine622, Threonine644, Serine732, Serine743, Serine785 and Serine962 to Alanine residues 2026-08-15 01:15:44 0
EBL1-bio
 
Resource Report
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RRID:Addgene_47792 Codon-optimised EBL1 Synthetic Ampicillin PMID:24043421 Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Exogenous signal peptide of mouse origin, changed Serine37, Serine53, Serine150, Serine255, Threonine313, Serine722, Serine845, Serine957, Serine1010, Serine1175, Serine1220, Serine1239, Serine1325, Threonine1346, Serine1352, Serine1606, Threonine1798, Serine1829, Serine2161, Serine2310, Serine2356, Serine2403, Threonine2426, Threonine2432, Serine2480, Serine2508, Threonine2518, Serine2560 and Threonine2573 to Alanine residues 2026-08-15 01:15:45 0
pMB66
 
Resource Report
Resource Website
RRID:Addgene_47946 Cas9 Synthetic Ampicillin PMID:23979586 To generate the unique SpeI site, a short, 37 bp, linker was inserted into an existing NdeI site. This linker seems to have been inserted 2 or more times, creating a short repeat region that we have been unable to sequence across. Sequencing with the M13 reverse primer will fail. Backbone Marker:Stratagene; Backbone Size:2958; Vector Backbone:pBluescript SK+; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:15:46 0
pMB62
 
Resource Report
Resource Website
RRID:Addgene_47944 Cas9 Synthetic Ampicillin PMID:23979586 To generate the unique SpeI site, a short, 37 bp, linker was inserted into an existing NdeI site. This linker seems to have been inserted 2 or more times, creating a short repeat region that we have been unable to sequence across. Sequencing with the M13 reverse primer will fail. Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3 Backbone Marker:Stratagene; Backbone Size:2958; Vector Backbone:pBluescript SK+; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:15:47 0
pMB63
 
Resource Report
Resource Website
RRID:Addgene_47945 Cas9 Synthetic Ampicillin PMID:23979586 To generate the unique SpeI site, a short, 37 bp, linker was inserted into an existing NdeI site. This linker seems to have been inserted 2 or more times, creating a short repeat region that we have been unable to sequence across. Sequencing with the M13 reverse primer will fail. Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3 Backbone Marker:Stratagene; Backbone Size:2958; Vector Backbone:pBluescript SK+; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:15:46 0
pMB70
 
Resource Report
Resource Website
RRID:Addgene_47943 guide RNA Synthetic Kanamycin PMID:23979586 Backbone Marker:Geneart; Vector Backbone:pMK; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Kanamycin 2026-08-15 01:15:46 0
pIK86
 
Resource Report
Resource Website
RRID:Addgene_47933 Cas9 Synthetic Ampicillin PMID:23979578 Backbone Size:2730; Vector Backbone:pUC57; Vector Types:CRISPR, Unspecified; Bacterial Resistance:Ampicillin 2026-08-15 01:15:45 0
pcDNA-T1ER
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_47928 T1ER Synthetic Ampicillin PMID:23838183 Ratiometric calcium sensor targeted to ER with KDEL sequence. The CFP fragment of Addgene plasmid 36325 pcDNA-D1ER was replaced with the brighter fluorescent protein mTurquoise. Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin KDEL 2026-08-15 01:15:47 2
pCS2-nCas9n
 
Resource Report
Resource Website
100+ mentions
RRID:Addgene_47929 nls-zcas9-nls Synthetic Ampicillin PMID:23918387 Note from depositor: Although this plasmid produces smaller transcripts in addition to the expected one, the product mixture displays activity comparable to that from pT3TS-nCas9n. For more information on Chen and Wente Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Chen/ Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:15:45 103
pFAB1935
 
Resource Report
Resource Website
RRID:Addgene_47860 his_T_min_linker_crp_T_min double terminator Synthetic Kanamycin PMID:23474465 Terminator efficiency measured at 99% (97% and 73% alone, respectively). Terminator inserted between RFP and GFP and flanked by Rnase III sites. RNase sites have sequences GTGATAGACTCAAGGTCGCTCCTAGCGAGTGGCCTTTATGATTATCAC and AGAGGGACAAACTCAAGGTCATTCGCAAGAGTGGCCTTTATGATTGACCTTCT, respectively. Terminator can be amplified by PCR with or without these sites, as desired. Backbone Size:3967; Vector Backbone:pFAB777; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin concatenate of his_min and crp_min 2026-08-15 01:15:46 0

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