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Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Scott Sternson; Backbone Size:5088; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: The plasmid is fully sequenced in the coding sequence regions (fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.
In addition, the published CaMKII promoter contains 10 basepair differences vs. the wild-type, and that persists here.
Proper citation: RRID:Addgene_64545 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: g1 gRNA
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pRS425; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23977949
Proper citation: RRID:Addgene_64387 Copy
Species: Other
Genetic Insert: MCP
Vector Backbone Description: Vector Backbone:pHAGE-UbiC; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25792328
Proper citation: RRID:Addgene_64541 Copy
Species: Synthetic
Genetic Insert: 12x PP7 stem-loops
Vector Backbone Description: Vector Backbone:pSF4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25792328
Proper citation: RRID:Addgene_64544 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yeast enhanced GFP
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23977949
Proper citation: RRID:Addgene_64389 Copy
Species: Other
Genetic Insert: MCP
Vector Backbone Description: Vector Backbone:pHAGE-UbiC; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25792328
Proper citation: RRID:Addgene_64540 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: c7 gRNA
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pRS425; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23977949
Proper citation: RRID:Addgene_64385 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yeast enhanced BFP2
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23977949
Proper citation: RRID:Addgene_64390 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yeast enhanced BFP2
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23977949
Proper citation: RRID:Addgene_64393 Copy
Species: E. coli
Genetic Insert: BirA
Vector Backbone Description: Backbone Marker:Yves Durocher; Backbone Size:6000; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18296487
Proper citation: RRID:Addgene_64395 Copy
Vector Backbone Description: Backbone Marker:Robert Gnuegge; Backbone Size:5364; Vector Backbone:pRG201; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26647923
Proper citation: RRID:Addgene_64394 Copy
Genetic Insert: MKIIK11A::nlsLexADBDo
Vector Backbone Description: Backbone Marker:see publication for details; Vector Backbone:pattB-nsyb; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25961791
Proper citation: RRID:Addgene_64726 Copy
Genetic Insert: MKII::nlsLexADBDo
Vector Backbone Description: Backbone Marker:see publication for details; Vector Backbone:pattB-nsyb; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25961791
Proper citation: RRID:Addgene_64725 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AKAR4-NES
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21807900
Proper citation: RRID:Addgene_64727 Copy
Genetic Insert: MKIIK11A::GAL4DBDo
Vector Backbone Description: Backbone Marker:see publication for details; Vector Backbone:pattB-nsyb; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25961791
Proper citation: RRID:Addgene_64721 Copy
Genetic Insert: MKII::GAL4DBDo
Vector Backbone Description: Backbone Marker:see publication for details; Vector Backbone:pQUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25961791
Proper citation: RRID:Addgene_64723 Copy
Genetic Insert: mCitrine V163A
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pKT; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24189399
Proper citation: RRID:Addgene_64685 Copy
Species: Homo sapiens
Genetic Insert: CTNNB1
Vector Backbone Description: Backbone Marker:John Doench/Kathleen Ottina; Backbone Size:9240; Vector Backbone:pcw107 (V5); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25538079
Comments: TCAG (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-E3-V5
Proper citation: RRID:Addgene_64616 Copy
Species: Homo sapiens
Genetic Insert: CTNNB1
Vector Backbone Description: Backbone Marker:John Doench/Kathleen Ottina; Backbone Size:9240; Vector Backbone:pcw107 (V5); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25538079
Comments: TCAG (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-E3-STOP
Proper citation: RRID:Addgene_64615 Copy
Species: Other
Genetic Insert: Map2k7 linker MAPK9
Vector Backbone Description: Backbone Marker:John Doench/Kathleen Ottina; Backbone Size:9240; Vector Backbone:pcw107 (V5); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25538079
Comments: GGCG (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-F2-STOP
Proper citation: RRID:Addgene_64618 Copy
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