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Species: Homo sapiens
Genetic Insert: CFP(1-158)-gamma-7
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4800; Vector Backbone:pcDNAI/Amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16641313
Proper citation: RRID:Addgene_55618 Copy
Species: Homo sapiens
Genetic Insert: 3xFLAG tagged HERC2 (C4762S)
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:5137; Vector Backbone:pcDNA5 FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24855649
Comments: There is a unique XhoI between the N-terminal 3xFLAG/STREP tag and the beginning of the HERC2 ORF. When cut with XhoI, PmeI, the untagged HERC2 ORF can be excised.
There is a TEV-protease site inserted between the N-terminal 3xFLAG/STREP tag and the HERC2 ORF.
Silent mutations have been incorporated into the ORF of HERC2 to render it refractory to the shRNA described in the associated publication.
Proper citation: RRID:Addgene_55614 Copy
Species: Homo sapiens
Genetic Insert: Sequestosome1
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mTurquoise-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 434; Emission = 474
Proper citation: RRID:Addgene_55581 Copy
Species: Homo sapiens
Genetic Insert: Zyxin
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mTurquoise; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 434; Emission = 474
Proper citation: RRID:Addgene_55589 Copy
Species: Homo sapiens
Genetic Insert: ZO1
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mTurquoise-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 434; Emission = 474
Proper citation: RRID:Addgene_55588 Copy
Species: Homo sapiens
Genetic Insert: Vimentin
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mTurquoise; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 434; Emission = 474
Proper citation: RRID:Addgene_55586 Copy
Species: Homo sapiens
Genetic Insert: Tubulin
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mTurquoise-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 434; Emission = 474
Proper citation: RRID:Addgene_55584 Copy
Species: Homo sapiens
Genetic Insert: Cer(1-158)-gamma-10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4800; Vector Backbone:pcDNAI/Amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16641313
Comments: Reference for monomeric Cerulean:
Rizzo MA, Springer GH, Granada B, Piston DW. An improved cyan fluorescent protein variant useful for FRET. Nat Biotechnol. 2004;22(4):445-9. PubMed PMID: 14990965.
Proper citation: RRID:Addgene_55627 Copy
Species: Homo sapiens
Genetic Insert: Cer(1-158)-gamma-7
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4800; Vector Backbone:pcDNAI/Amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16641313
Comments: Reference for monomeric Cerulean:
Rizzo MA, Springer GH, Granada B, Piston DW. An improved cyan fluorescent protein variant useful for FRET. Nat Biotechnol. 2004;22(4):445-9. PubMed PMID: 14990965.
Proper citation: RRID:Addgene_55626 Copy
Species: Homo sapiens
Genetic Insert: ATG14
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mTurquoise-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 434; Emission = 474
Proper citation: RRID:Addgene_55534 Copy
Species: Homo sapiens
Genetic Insert: Calnexin
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mTurquoise-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 434; Emission = 474
Proper citation: RRID:Addgene_55539 Copy
Species: Homo sapiens
Genetic Insert: Clathrin
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mTurquoise; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 434; Emission = 474
Proper citation: RRID:Addgene_55544 Copy
Species: Homo sapiens
Genetic Insert: CENPA
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mTurquoise-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 434; Emission = 474
Proper citation: RRID:Addgene_55542 Copy
Species: Homo sapiens
Genetic Insert: Caveolin
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mTurquoise-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 434; Emission = 474
Proper citation: RRID:Addgene_55540 Copy
Species: Homo sapiens
Genetic Insert: Endo
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mTurquoise; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 434; Emission = 474
Proper citation: RRID:Addgene_55549 Copy
Species: Homo sapiens
Genetic Insert: EB3
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mTurquoise; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 434; Emission = 474
Proper citation: RRID:Addgene_55548 Copy
Species: Homo sapiens
Genetic Insert: FYN2
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mTurquoise-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 434; Emission = 434
Proper citation: RRID:Addgene_55554 Copy
Species: Homo sapiens
Genetic Insert: FYN
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mTurquoise-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 434; Emission = 434
Proper citation: RRID:Addgene_55553 Copy
Species: Homo sapiens
Genetic Insert: Fibrillarin
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mTurquoise; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 434; Emission = 434
Proper citation: RRID:Addgene_55552 Copy
Species: Homo sapiens
Genetic Insert: Farnesyl
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mTurquoise; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 434; Emission = 474
Proper citation: RRID:Addgene_55551 Copy
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