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Species: Rattus norvegicus
Genetic Insert: S6K1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pRK7; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11967149
Comments: See Schalm et. al., 2005 JBC Vol. 280(12)11101-11106.
Proper citation: RRID:Addgene_8991 Copy
Species: Rattus norvegicus
Genetic Insert: S6K1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pRK7; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11967149
Proper citation: RRID:Addgene_8990 Copy
Species: Rattus norvegicus
Genetic Insert: Cacna2d1
Vector Backbone Description: Backbone Size:5129; Vector Backbone:pMT2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27782881
Comments: 5 amino acids are mutated to valines in the region believe to be involved in post translational cleavage of the alpha2 and delta-1 moities (there is already a Valine at AA position 946) leading to a stretch of 6 valines
Proper citation: RRID:Addgene_89894 Copy
Species: Rattus norvegicus
Genetic Insert: Cacnb1
Vector Backbone Description: Backbone Size:5129; Vector Backbone:pMT2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27489103
Comments: Cut out B1b with EcoRI and SpeI (but then you lose the start codon), cut out mCherry with SpeI and NotI (but then you lose the Stop codon). The mCherry is missing the N-terminal start codon. The SpeI site introduces 2 extra amino acids: Threonine and serine. Cut out the entire insert with EcoRI and NotI.
Proper citation: RRID:Addgene_89892 Copy
Species: Rattus norvegicus
Genetic Insert: S6K1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pRK7; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11967149
Proper citation: RRID:Addgene_8988 Copy
Species: Rattus norvegicus
Genetic Insert: ERK2
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16051177
Comments: DEF-domain docking mutant.
Proper citation: RRID:Addgene_8978 Copy
Species: Rattus norvegicus
Genetic Insert: ERK2
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16051177
Comments: DEF-domain docking mutant.
Proper citation: RRID:Addgene_8977 Copy
Species: Rattus norvegicus
Genetic Insert: ERK2
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16051177
Comments: DEF-domain docking mutant.
Proper citation: RRID:Addgene_8976 Copy
Species: Rattus norvegicus
Genetic Insert: ALK7
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10980615
Proper citation: RRID:Addgene_80887 Copy
Species: Rattus norvegicus
Genetic Insert: GluN1
Vector Backbone Description: Backbone Marker:Addgene#61463; Vector Backbone:pAAV-CW3SL-EGFP; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34977503
Proper citation: RRID:Addgene_180173 Copy
Species: Rattus norvegicus
Genetic Insert: GluN1
Vector Backbone Description: Backbone Marker:Addgene#61463; Vector Backbone:pAAV-CW3SL-EGFP; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34977503
Proper citation: RRID:Addgene_180174 Copy
Species: Rattus norvegicus
Genetic Insert: GluN1
Vector Backbone Description: Backbone Marker:Addgene #82612; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34977503
Proper citation: RRID:Addgene_180175 Copy
Species: Rattus norvegicus
Genetic Insert: neu NT
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBR322; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2898299
Comments: See scanned map.
(Leder #C-172)
Proper citation: RRID:Addgene_1823 Copy
Species: Rattus norvegicus
Genetic Insert: TrkB
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pRC/CMV AC7 vector, a derivative of the pRC/CMV vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9927421
Comments: cloned in the pRC/CMV AC7 vector, a derivative of the pRC/CMV vector (Invitrogen), which contains the BM40 signal peptide
Please note that the endogenous signal sequence of TrkB was replaced by the BM40 signal peptide sequence (MRAWIFFLLCLAGRALAAPLA). When compared to translated sequences for GenBank ID M55291.1 or NM_012731.2, the TrkB protein sequence starts at amino acid #32
Proper citation: RRID:Addgene_39978 Copy
Species: Rattus norvegicus
Genetic Insert: SAPAP3
Vector Backbone Description: Vector Backbone:pCMV5 (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9115257
Proper citation: RRID:Addgene_40217 Copy
Species: Rattus norvegicus
Genetic Insert: SAPAP1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCI-neo Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9115257
Comments: Please note that after the stop codon of SAPAP1 and before the NotI site Addgene's sequencing results identified an untranslated portion of Cnksr2 (amino acids 850-1028 when compared GenBank ID NP_001106837.1)
Proper citation: RRID:Addgene_40215 Copy
Species: Rattus norvegicus
Genetic Insert: Clta
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17956594
Proper citation: RRID:Addgene_40271 Copy
Species: Rattus norvegicus
Genetic Insert: Camuiα-CR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5320; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22961245
Comments: Venus was replaced with GFP Clover and CFP S175G was replaced with RFP mRuby2.
Proper citation: RRID:Addgene_40256 Copy
Species: Rattus norvegicus
Genetic Insert: GCaMP6f
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3955; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23868258
Proper citation: RRID:Addgene_40755 Copy
Species: Rattus norvegicus
Genetic Insert: GCaMP6m
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3955; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23868258
Proper citation: RRID:Addgene_40754 Copy
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