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Vector Backbone Description: Vector Backbone:pMA2780; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128043 Copy
Vector Backbone Description: Vector Backbone:pLEX Mcs; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128048 Copy
Vector Backbone Description: Vector Backbone:pcdna3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128047 Copy
Vector Backbone Description: Vector Backbone:pcdna3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128042 Copy
Vector Backbone Description: Vector Backbone:pcdna3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128040 Copy
Vector Backbone Description: Vector Backbone:pcdna3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128032 Copy
Species: Synthetic
Genetic Insert: Cpf1
Vector Backbone Description: Backbone Size:10152; Vector Backbone:pAC575; Vector Types:Bacterial Expression, CRISPR, Fungal Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31061713
Comments: AMA1 sequence contains multiple ambiguous bases that are likely mixed nucleotide populations. AMA1 is a palindrome and thus difficult to sequence. Dep. confirms the mixed population does not affect plasmid function.
Proper citation: RRID:Addgene_127984 Copy
Vector Backbone Description: Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128038 Copy
Species: Synthetic
Genetic Insert: Cpf1
Vector Backbone Description: Backbone Size:10868; Vector Backbone:pAC573; Vector Types:Bacterial Expression, CRISPR, Fungal Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31061713
Proper citation: RRID:Addgene_127982 Copy
Vector Backbone Description: Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128037 Copy
Species: Synthetic
Genetic Insert: Cpf1
Vector Backbone Description: Backbone Size:10239; Vector Backbone:pAC572; Vector Types:Bacterial Expression, CRISPR, Fungal Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31061713
Comments: AMA1 sequence contains multiple ambiguous bases that are likely mixed nucleotide populations. AMA1 is a palindrome and thus difficult to sequence. Dep. confirms the mixed population does not affect plasmid function.
Proper citation: RRID:Addgene_127981 Copy
Vector Backbone Description: Vector Backbone:pcdna3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128031 Copy
Vector Backbone Description: Vector Backbone:pcdna3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128030 Copy
Vector Backbone Description: Vector Backbone:pcdna3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128023 Copy
Species: Aspergillus fumigatus
Genetic Insert: pyrG
Vector Backbone Description: Vector Backbone:custom; Vector Types:Bacterial Expression, Fungal Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31061713
Comments: AMA1 is a palindrome which causes issues sequencing. There are a few nucleotides with a mixed population. This however does not affect plasmid function.
Published in: Nødvig CS, Hoof JB, Kogle ME, Jarczynska ZD, Lehmbeck J, Klitgaard DK, et al. Efficient oligo nucleotide mediated CRISPR-Cas9 gene editing in Aspergilli. Fungal Genet Biol. 2018;115:78–89, https://doi.org/10.1016/j.fgb.2018.01.004.
Proper citation: RRID:Addgene_127973 Copy
Vector Backbone Description: Vector Backbone:pcdna3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128028 Copy
Species: Streptoalloteichus hindustanus
Genetic Insert: ble (bleomycin resistance marker)
Vector Backbone Description: Vector Backbone:custom; Vector Types:Bacterial Expression, Fungal Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31061713
Comments: AMA1 sequence contains multiple ambiguous bases that are likely mixed nucleotide populations. AMA1 is a palindrome and thus difficult to sequence. Dep. confirms the mixed population does not affect plasmid function.
Proper citation: RRID:Addgene_127976 Copy
Species: Synthetic
Genetic Insert: Green fluorescence protein
Vector Backbone Description: Backbone Marker:Takara; Backbone Size:2679; Vector Backbone:pMD19 T-Vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31963386
Proper citation: RRID:Addgene_128141 Copy
Species: From Acinetobacter johnsonii SH046, codon optimized for E. coli
Genetic Insert: vdh
Vector Backbone Description: Backbone Size:2987; Vector Backbone:pBEAST; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31462649
Proper citation: RRID:Addgene_128134 Copy
Species: Homo sapiens
Genetic Insert: sgRNA against human TSC2
Vector Backbone Description: Backbone Marker:Feng Zhang; Backbone Size:9271; Vector Backbone:PX458 (pSpCas9(BB)-2A-GFP); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29861175
Proper citation: RRID:Addgene_128098 Copy
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