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Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Addgene: 85866; Vector Backbone:AB.pCCL.sin.cPPT.GFP.miR-17-3p.sensor.PGK.dNGFR.WPRE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30778069
Comments: Bi-directional expression vector driving EGFP under control of a miR-17 MRE with NGFR as an internal control
Proper citation: RRID:Addgene_124691 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Addgene: 85866; Vector Backbone:AB.pCCL.sin.cPPT.GFP.miR-17-3p.sensor.PGK.dNGFR.WPRE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30778069
Comments: Bi-directional expression vector driving EGFP under control of a miR-17 MRE with NGFR as an internal control
Proper citation: RRID:Addgene_124692 Copy
Species: Other
Genetic Insert: Ptet
Vector Backbone Description: Backbone Size:3729; Vector Backbone:pLZ12; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31377338
Proper citation: RRID:Addgene_124768 Copy
Species: Other
Genetic Insert: Ptet
Vector Backbone Description: Backbone Size:3729; Vector Backbone:pLZ12; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31377338
Proper citation: RRID:Addgene_124769 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pCold I; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31069270
Proper citation: RRID:Addgene_124890 Copy
Species: Other
Genetic Insert: Cas12a
Vector Backbone Description: Vector Backbone:pCold I; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31069270
Proper citation: RRID:Addgene_124891 Copy
Species: Other
Genetic Insert: LacZ
Vector Backbone Description: Vector Backbone:AV0.1-CS; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_124968 Copy
Species: Other
Genetic Insert: ENO1 promoter
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Contains the appropriate YTK-compatible Golden Gate overhangs for a type 2 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125036 Copy
Species: Other
Genetic Insert: INU1 promoter
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Contains the appropriate YTK-compatible Golden Gate overhangs for a type 2 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125039 Copy
Species: Other
Genetic Insert: TDH1 promoter
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Contains the appropriate YTK-compatible Golden Gate overhangs for a type 2 part
Please note: Addgene NGS finds two ambiguous bases within the KmTDH1pr sequence. It is not known if these regions contain regulatory elements. Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125037 Copy
Species: Other
Genetic Insert: mNeon/mScarletP2A Rac1-V12
Vector Backbone Description: Backbone Size:6734; Vector Backbone:pCax; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508591
Comments: Intent is to introduce low doses of pCAG Cre to recombine a subset of cells, such that mNeon can act as an internal control to mScarlet for use in in utero electroporation or other related techniques. Rac1-V12 is expressed following a P2A site, so that Cre+ cells will be both mScarlet positive and also contain an untagged Rac1-V12.
Note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to the depositor's sequence for this section of the plasmid.
Proper citation: RRID:Addgene_125136 Copy
Species: Other
Genetic Insert: mNeon/mScarletP2A Rac1-N17
Vector Backbone Description: Backbone Size:6734; Vector Backbone:pCax; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508591
Comments: Intent is to introduce low doses of pCAG Cre to recombine a subset of cells, such that mNeon can act as an internal control to mScarlet for use in in utero electroporation or other related techniques. Rac1-N17 is expressed following a P2A site, so that Cre+ cells will be both mScarlet positive and also contain an untagged Rac1-N17.
Note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to the depositor's sequence for this section of the plasmid.
Proper citation: RRID:Addgene_125137 Copy
Species: Other
Genetic Insert: FAR protein
Vector Backbone Description: Backbone Size:6457; Vector Backbone:piggyback; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_125127 Copy
Species: Other
Genetic Insert: FAR protein
Vector Backbone Description: Backbone Size:6457; Vector Backbone:piggyback; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_125128 Copy
Species: Other
Genetic Insert: FAR protein
Vector Backbone Description: Backbone Size:6457; Vector Backbone:piggyback; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_125126 Copy
Species: Other
Genetic Insert: FAR protein
Vector Backbone Description: Backbone Size:6457; Vector Backbone:piggyback; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_125129 Copy
Species: Other
Genetic Insert: Right homology arm for targeting integration to chromosome V: 23743..21744
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Meant for the construction of integrative vectors (single- or multi-gene). Contains the appropriate YTK-compatible Golden Gate overhangs for a type 1 part. The necessary internal BsmBI sites to use this as a connector for multi-gene assembly have been added by PCR. Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125064 Copy
Species: Other
Genetic Insert: KmARS2
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Contains the appropriate YTK-compatible Golden Gate overhangs for a type 7 part.
Please Note: Some of the bases in the native sequence were modified to eliminate an endogeneous BsaI site that interfered with Golden Gate cloning, but plasmids using the modified ARS have been stable so far in experiments. Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125062 Copy
Species: Other
Genetic Insert: KmARS/CEN6
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Contains the appropriate YTK-compatible Golden Gate overhangs for a type 7 part. Equivalent to the ARS2/CEN sequence from strain ATCC 12424
Please note: Addgene NGS finds a mixed population (C/T) at single nucleotide in KmARS/Cen6. Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125061 Copy
Species: Other
Genetic Insert: INU1 terminator
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Contains the appropriate YTK-compatible Golden Gate overhangs for a type 4 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125053 Copy
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