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Species: Mus musculus
Genetic Insert: tBid-4E
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35442739
Comments: Transfection of this plasmid will express "VtBid-4E" (Venus fused to the N-terminus of the BH3-only protein, truncated Bid (tBid)) in live cells. In cells, caspase 8 cleavage of Bid produces the p15 fragment, referred to as tBid. BH3 region has hydrophobic positions H1-H4 mutated to glutamic acid (E). BH3, Mutations: I27E, L31E, I34E, M38E. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_166743 Copy
Species: Mus musculus
Genetic Insert: BimEL-4E
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30860026
Comments: Transfection of this plasmid will express "VBimEL-4E" (Venus fused to the N-terminus of the BH3-only protein, BimEL-4E) in live cells. There is a 3 amino acid flexible linker between Venus and BimEL-4E proteins. BH3 region has hydrophobic positions H1-H4 mutated to glutamic acid (E), refered to as "BH3-4E" mutation, BH3 mutations: I146E, L150E, I153E, F157E. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_166742 Copy
Species: Homo sapiens
Genetic Insert: Sec31
Vector Backbone Description: Vector Backbone:Halo-C; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33852913
Proper citation: RRID:Addgene_166895 Copy
Species: Homo sapiens
Genetic Insert: SEC23A
Vector Backbone Description: Vector Backbone:mCherry-C; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33852913
Proper citation: RRID:Addgene_166894 Copy
Species: Homo sapiens
Genetic Insert: SEC23A
Vector Backbone Description: Vector Backbone:mEmerald-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33852913
Proper citation: RRID:Addgene_166893 Copy
Species: Homo sapiens
Genetic Insert: SEC23A
Vector Backbone Description: Vector Backbone:Halo-C; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33852913
Proper citation: RRID:Addgene_166892 Copy
Species: hamster
Genetic Insert: Sar1b-H79G
Vector Backbone Description: Vector Backbone:YFP-N; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33852913
Proper citation: RRID:Addgene_166898 Copy
Species: Rattus norvegicus
Genetic Insert: p58
Vector Backbone Description: Vector Backbone:Halo; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33852913
Comments: Depositor confirms mutations in p58 (L405Q, R419S) do not affect plasmid function.
Proper citation: RRID:Addgene_166896 Copy
Species: Mus musculus
Genetic Insert: VAMP2
Vector Backbone Description: Vector Backbone:EGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33658493
Proper citation: RRID:Addgene_166891 Copy
Species: Mus musculus
Genetic Insert: VAMP2
Vector Backbone Description: Vector Backbone:EGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33658493
Proper citation: RRID:Addgene_166890 Copy
Species: Homo sapiens
Genetic Insert: NLRP1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33731932
Comments: Gateway pDONR vector encoding NLRP1 with the patient mutation M77V (Zhong et al., 2016) (no stop codon)
Proper citation: RRID:Addgene_166818 Copy
Vector Backbone Description: Backbone Marker:Rock Lab; Backbone Size:7670; Vector Backbone:plRL2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34297925
Proper citation: RRID:Addgene_166886 Copy
Species: Homo sapiens
Genetic Insert: NLRP1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33731932
Comments: Gateway pDONR vector containing NLRP1 FIIND with autoprocessing deficient S1213A mutation (no stop codon)
Proper citation: RRID:Addgene_166832 Copy
Species: Homo sapiens
Genetic Insert: NLRP1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33731932
Comments: Gateway pDONR vector containing NLRP1 FIIND (no stop codon)
Proper citation: RRID:Addgene_166831 Copy
Species: Homo sapiens
Genetic Insert: DPP9
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33731932
Comments: pDONR gateway shuttling vector with DPP9S (no stop codon)
Proper citation: RRID:Addgene_166789 Copy
Species: Homo sapiens
Genetic Insert: NLRP1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33731932
Comments: Gateway pDONR vector encoding NLRP1 with the patient mutation _787-843 (Zhong et al., 2016) (no stop codon)
Proper citation: RRID:Addgene_166821 Copy
Species: Homo sapiens
Genetic Insert: NLRP1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33731932
Comments: Gateway pDONR vector encoding NLRP1 with the patient mutation T755N (Drutman et al., 2019) (no stop codon)
Proper citation: RRID:Addgene_166820 Copy
Species: Homo sapiens
Genetic Insert: NLRP1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33731932
Comments: Gateway pDONR vector containing NLRP1 with the P1214R patient mutation (no stop codon)
Proper citation: RRID:Addgene_166824 Copy
Species: Homo sapiens
Genetic Insert: NLRP1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33731932
Comments: Gateway pDONR vector containing NLRP1 (no stop codon)
Proper citation: RRID:Addgene_166823 Copy
Species: Homo sapiens
Genetic Insert: NLRP1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33731932
Comments: pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1215 N-terminus (no stop)
Proper citation: RRID:Addgene_166828 Copy
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