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Species: Homo sapiens
Genetic Insert: KDR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21107320
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_23925 Copy
Species: Homo sapiens
Genetic Insert: TEC
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21107320
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_23885 Copy
Species: Homo sapiens
Genetic Insert: ERBB2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21107320
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_23888 Copy
Species: Homo sapiens
Genetic Insert: ITK
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other, Gateway Donor vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21107320
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_23881 Copy
Species: Homo sapiens
Genetic Insert: PKMYT1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21107320
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_23884 Copy
Vector Backbone Description: Backbone Size:5028; Vector Backbone:pMOTag; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin
Comments: pMOTag4H-crelox-PUR made by Jenny Li taking the HindIII-BamHI crelox-PUR fragment from pyrFEKO-PUR and inserting into HindIII-BamHI digested pMOTag4H.
Proper citation: RRID:Addgene_24028 Copy
Vector Backbone Description: Backbone Size:5626; Vector Backbone:pMOTag; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin
Comments: pMOTag4HM-crelox-HYG made by Jenny Li taking the HindIII-BamHI crelox-HYG fragment from pyrFEKO-HYG and inserting into HindIII-BamHI digested pMOTag4HM made by Jessica.
Proper citation: RRID:Addgene_24029 Copy
Vector Backbone Description: Backbone Size:5553; Vector Backbone:pyrFEKO; Vector Types:Floxed targeting vector for Trypanosome; Bacterial Resistance:Ampicillin
Comments: Unpublished (variant of constructs described in Scahill et al 2008).
For more information about using this vector, please see: http://tryps.rockefeller.edu/trypsru2_cre-lox.html
Proper citation: RRID:Addgene_24024 Copy
Vector Backbone Description: Backbone Size:5414; Vector Backbone:pMOTag; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin
Comments: pMOTag2T-crelox-HYG made by Jenny Li taking the HindIII-BamHI crelox-HYG fragment from pyrFEKO-HYG and inserting into HindIII-BamHI digested pMOTag2T made by Luisa.
Based on pMOTag series made in pBluescript II KS+
Proper citation: RRID:Addgene_24025 Copy
Vector Backbone Description: Backbone Size:4976; Vector Backbone:pMOTag; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin
Comments: pMOTag2T-crelox-PUR made by Jenny Li taking the HindIII-BamHI crelox-PUR fragment from pyrFEKO-PUR and inserting into HindIII-BamHI digested pMOTag2T made by Luisa.
Based on pMOTag series made in pBluescript II KS+
Proper citation: RRID:Addgene_24026 Copy
Vector Backbone Description: Backbone Size:5467; Vector Backbone:pMOTag; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin
Comments: pMOTag4H-crelox-HYG made by Jenny Li taking the HindIII-BamHI crelox-HYG fragment from pyrFEKO-HYG and inserting into HindIII-BamHI digested pMOTag4H.
Based on pMOTag series made in pBluescript II KS+
Proper citation: RRID:Addgene_24027 Copy
Vector Backbone Description: Backbone Size:6191; Vector Backbone:pyrFEKO; Vector Types:Floxed targeting vector for Trypanosome; Bacterial Resistance:Ampicillin
Comments: Unpublished (variant of constructs described in Scahill et al 2008).
For more information about using this vector, please see: http://tryps.rockefeller.edu/trypsru2_cre-lox.html
Proper citation: RRID:Addgene_24020 Copy
Vector Backbone Description: Backbone Size:5753; Vector Backbone:pyrFEKO; Vector Types:Floxed targeting vector for Trypanosome; Bacterial Resistance:Ampicillin
Comments: Unpublished (variant of constructs described in Scahill et al 2008).
For more information about using this vector, please see: http://tryps.rockefeller.edu/trypsru2_cre-lox.html
Proper citation: RRID:Addgene_24021 Copy
Vector Backbone Description: Backbone Size:6022; Vector Backbone:pyrFEKO; Vector Types:Floxed targeting vector for Trypanosome; Bacterial Resistance:Ampicillin
Comments: Unpublished (variant of constructs described in Scahill et al 2008).
For more information about using this vector, please see: http://tryps.rockefeller.edu/trypsru2_cre-lox.html
Proper citation: RRID:Addgene_24022 Copy
Species: Homo sapiens
Genetic Insert: NFATc1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCR4; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16740479
Comments: The c1nuc plasmid expresses the constitutively nuclear form of NFATc1 (Ho et al Nature 2004). It contains mutations in the phosphorylation sites for Dyrk1a and GSK3 that are responsible for actively exporting the protein from the nucleus (Beal et al Science 1997; Arron et al Nature 2006). It can be used as a transgene to explore NFAT signaling (Winslow et al Dev Cell 2006) and when expressed in a specific tissue can direct events such as bone development, thymocyte development or pancreatic beta cell functions (Heit J et al Nature 2007).
Proper citation: RRID:Addgene_23988 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL25
Vector Backbone Description: Backbone Size:5000; Vector Backbone:yEGFP3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Entire RPL25 ORF inserted upstream of eGFP in EcoRI/HindIII of yEGFP3
Proper citation: RRID:Addgene_24037 Copy
Vector Backbone Description: Backbone Size:5099; Vector Backbone:pMOTag; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin
Comments: pMOTag43M-crelox-PUR made by Jenny Li taking the HindIII-BamHI crelox-PUR fragment from pyrFEKO-PUR and inserting into HindIII-BamHI digested pMOTag43M.
Based on pMOTag series made in pBluescript II KS+
Proper citation: RRID:Addgene_24032 Copy
Vector Backbone Description: Backbone Size:5188; Vector Backbone:pMOTag; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin
Comments: pMOTag4HM-crelox-PUR made by Jenny Li taking the HindIII-BamHI crelox-PUR fragment from pyrFEKO-PUR and inserting into HindIII-BamHI digested pMOTag4HM made by Jessica.
Proper citation: RRID:Addgene_24030 Copy
Genetic Insert: T7 RNA Polymerase
Vector Backbone Description: Backbone Size:6000; Vector Backbone:N/A; Vector Types:Trypanosome Expression; Bacterial Resistance:Ampicillin
Comments: T7 RNA Polymerase and Tet Repressor present (see map).
Target locus: Tubulin
Regulation: None
Proper citation: RRID:Addgene_24007 Copy
Species: Rattus norvegicus
Genetic Insert: GluR1
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pSIN; Vector Types:Sindbis virus production; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19543281
Proper citation: RRID:Addgene_24004 Copy
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