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Species: Synthetic
Genetic Insert: 5xUAS
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34525356
Comments: Enhancer
Proper citation: RRID:Addgene_165891 Copy
Species: Synthetic
Genetic Insert: LiCre
Vector Backbone Description: Backbone Marker:Euroscarf; Vector Backbone:pSH63; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33620312
Comments: Please note: Plasmid contains 6 nucleotide discrepancies in the Met17 promoter sequence. These discrepancies are not known to affect plasmid function.
Proper citation: RRID:Addgene_166661 Copy
Species: Synthetic
Genetic Insert: LiCre
Vector Backbone Description: Backbone Marker:Euroscarf; Vector Backbone:pSH63; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33620312
Proper citation: RRID:Addgene_166660 Copy
Species: Synthetic
Genetic Insert: EGFP-2A-Tetanus Toxin
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33704065
Proper citation: RRID:Addgene_166611 Copy
Species: Synthetic
Genetic Insert: ChR2-TdTomato
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33704065
Proper citation: RRID:Addgene_166610 Copy
Species: Synthetic
Genetic Insert: TurboID
Vector Backbone Description: Vector Backbone:pDONR p2r-p3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33591275
Proper citation: RRID:Addgene_166570 Copy
Species: Synthetic
Genetic Insert: BASU
Vector Backbone Description: Vector Backbone:pDONR 221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33591275
Proper citation: RRID:Addgene_166565 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33704065
Proper citation: RRID:Addgene_166601 Copy
Species: Synthetic
Genetic Insert: GCaMP6s
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33704065
Proper citation: RRID:Addgene_166606 Copy
Species: Synthetic
Genetic Insert: EGFP-2A-Tetanus Toxin
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33704065
Proper citation: RRID:Addgene_166603 Copy
Species: Synthetic
Genetic Insert: rtTA
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33704065
Proper citation: RRID:Addgene_166598 Copy
Species: Synthetic
Genetic Insert: tTA
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33704065
Proper citation: RRID:Addgene_166597 Copy
Species: Synthetic
Genetic Insert: hM3Dq-mCherry
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33704065
Proper citation: RRID:Addgene_166599 Copy
Species: Synthetic
Genetic Insert: ActA tail anchor (Listeria monocytogenes)
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: "SGLRSRGSGLRSRAQASNSAVDARGSGLRSRAQASNSAVDARGSGLRSRAQASNSAVDAR
GSGLRSRAQASNSAVDARV" amino acid targeting sequence, which we refer to as "ActA", for the mitochondrial outer membrane. Pearson's correlation of 0.8-0.9 with mitotracker red signal in cells. "79aa" indicates the 79 amino acid flexible linker region between Venus and the ActA targeting signal.
Proper citation: RRID:Addgene_166763 Copy
Species: Synthetic
Genetic Insert: dCas12a-NLS
Vector Backbone Description: Backbone Size:7206; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34197613
Proper citation: RRID:Addgene_166731 Copy
Species: Synthetic
Genetic Insert: Venus
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35442739
Comments: Plasmid can be used to express monomeric Venus fluorophore in bacteria. We used E.coli BL21-AI (Arabinose induction) for protein production, induced 5h at 30oC. Purify protein via Nickel column chromatography. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_166756 Copy
Species: Synthetic
Genetic Insert: U6-VEGFA array presgRNA
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc.; Backbone Size:3315; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34178991
Proper citation: RRID:Addgene_166873 Copy
Species: Synthetic
Genetic Insert: U6-VEGFA presgRNA
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc.; Backbone Size:3316; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34178991
Proper citation: RRID:Addgene_166872 Copy
Species: Synthetic
Genetic Insert: U6-control presgRNA
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc.; Backbone Size:3316; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34178991
Proper citation: RRID:Addgene_166871 Copy
Species: Synthetic
Genetic Insert: SARS-COV-2 Nsp8
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34154407
Proper citation: RRID:Addgene_166861 Copy
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