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Species: Saccharomyces cerevisiae
Genetic Insert: tTDH1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 4b parts are terminators used when a type 4a has been included as a coding sequence. For more information, please visit the supplemental information in PMID:25871405.
This plasmid stock has been found to contain dimers. Dimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_65173 Copy
Species: Synthetic
Genetic Insert: ConRE
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 5 parts are 3' assembly connectors in the Duebery YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65179 Copy
Species: Synthetic
Genetic Insert: ConR3
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 5 parts are 3' assembly connectors in the Duebery YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65176 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HIS3
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65183 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LEU2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65182 Copy
Species: Synthetic
Genetic Insert: NourseothricinR
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65185 Copy
Species: Synthetic
Genetic Insert: KanamycinR
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65184 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: URA3
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65181 Copy
Species: Synthetic
Genetic Insert: ConRE'
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 5 parts are 3' assembly connectors in the Duebery YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65180 Copy
Species: Synthetic
Genetic Insert: ZeocinR
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65187 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CEN6/ARS4
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 7 and 8 parts are Yeast propagation machinery in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65188 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LEU2 3' Homology
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 7 and 8 parts are Yeast propagation machinery in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65194 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: URA3 3' Homology
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 7 and 8 parts are Yeast propagation machinery in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65193 Copy
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pKS013; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26900850
Proper citation: RRID:Addgene_65466 Copy
Genetic Insert: Ntag-mCherry
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26900850
Proper citation: RRID:Addgene_65465 Copy
Genetic Insert: MBP-Ntag-mCherry
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26900850
Proper citation: RRID:Addgene_65464 Copy
Species: Staphylococcus aureus
Genetic Insert: mammalian codon-optimized SaCas9, and SaCas9 gRNA
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26098369
Comments: Use BsaI sites to insert sgRNA spacer sequence into the vector.
Proper citation: RRID:Addgene_65770 Copy
Species: Other
Genetic Insert: mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26098369
Comments: Use BspMI sites to insert sgRNA spacer sequence into the vector.
Proper citation: RRID:Addgene_65769 Copy
Species: Other
Genetic Insert: mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26098369
Comments: Use BsaI sites to insert sgRNA spacer sequence into the vector.
Proper citation: RRID:Addgene_65768 Copy
Species: Arabidopsis thaliana
Genetic Insert: Cold temperature germinating 10 (CTG10)
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:6494; Vector Backbone:pBD-GAL4 Cam; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29632208
Proper citation: RRID:Addgene_67911 Copy
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