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Species: Synthetic
Genetic Insert: repA
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29371642
Proper citation: RRID:Addgene_109414 Copy
Species: Synthetic
Genetic Insert: repA
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29371642
Proper citation: RRID:Addgene_109415 Copy
Species: Synthetic
Genetic Insert: repA
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29371642
Proper citation: RRID:Addgene_109416 Copy
Genetic Insert: pJ23100mut-dCas9-T- phtpG1-sgRNA
Vector Backbone Description: Vector Backbone:pAZ16 ( Plasmid has got AmpR and p15A origin); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29578536
Proper citation: RRID:Addgene_109391 Copy
Genetic Insert: "capacity monitor" (https://www.addgene.org/66073/) has been inserted in the lambda phage integration site.
Vector Backbone Description: Vector Backbone:NCBI RefSeq NC_010473.1 for DH10B; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29578536
Proper citation: RRID:Addgene_109392 Copy
Genetic Insert: "capacity monitor" (https://www.addgene.org/66073/) has been inserted in the lambda phage integration site.
Vector Backbone Description: Vector Backbone:NCBI RefSeq NC_000913.3 for MG1655; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29578536
Proper citation: RRID:Addgene_109393 Copy
Vector Backbone Description: Backbone Size:7010; Vector Backbone:pAAV; Vector Types:Mammalian Expression, Mouse Targeting, AAV, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29503867
Proper citation: RRID:Addgene_109396 Copy
Genetic Insert: phtpG1-GFP
Vector Backbone Description: Vector Backbone:YTK095 (ColE1 origin of replication and AmpR); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29578536
Proper citation: RRID:Addgene_109387 Copy
Species: Synthetic
Genetic Insert: anti-GFP nanobody fused to a T7, APEX2, HA, BAP and His6 epitope
Vector Backbone Description: Backbone Marker:Merck - Novagen; Vector Backbone:pET24a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29915061
Proper citation: RRID:Addgene_109420 Copy
Genetic Insert: phtpG2-GFP
Vector Backbone Description: Vector Backbone:YTK095 (ColE1 origin of replication and AmpR); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29578536
Proper citation: RRID:Addgene_109388 Copy
Species: Synthetic
Genetic Insert: anti-GFP nanobody fused to a T7, mCherry, HA, BAP and His6 epitope
Vector Backbone Description: Backbone Marker:Merck - Novagen; Vector Backbone:pET24a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29915061
Proper citation: RRID:Addgene_109421 Copy
Species: Homo sapiens
Genetic Insert: Rod opsin
Vector Backbone Description: Backbone Size:5374; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23554393
Comments: Also see Ballister et al. BMC Biol. 2018 (doi: 10.1186/s12915-017-0475-2) for additional use.
Proper citation: RRID:Addgene_109361 Copy
Species: Homo sapiens
Genetic Insert: Gsz chimera
Vector Backbone Description: Backbone Size:6002; Vector Backbone:pEM705; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29338718
Proper citation: RRID:Addgene_109355 Copy
Species: Homo sapiens
Genetic Insert: Gsq chimera
Vector Backbone Description: Backbone Size:6002; Vector Backbone:pEM705; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29338718
Proper citation: RRID:Addgene_109356 Copy
Vector Backbone Description: Backbone Marker:Joung lab; Backbone Size:7636; Vector Backbone:pMLM3705 (Addgene plasmid #47754).; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29524148
Comments: The DNA fragment encoding the effector domain is synthesized by PCR and cloned between the AscI and PacI sites of pdCas9-NED to create in-frame fusion between dCas9 (D10A, H840A) and the effector domain. For experiments involving cell lines stably expressing the dCas9-effector domain fusion protein, the coding sequence of the effector domain can be excised from the pdCas9-NED-based recombinant plasmid and transferred into pHAGE EF1α dCas9-NED (Addgene plasmid #109369). Cloning the AscI-PacI fragment between the MluI and AsiSI sites of pHAGE EF1α dCas9-NED preserves the reading frame of the fusion protein (Goubert et al., 2018).
Proper citation: RRID:Addgene_109358 Copy
Species: Homo sapiens
Genetic Insert: Gs13 chimera
Vector Backbone Description: Backbone Size:6002; Vector Backbone:pEM705; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29338718
Proper citation: RRID:Addgene_109359 Copy
Species: Homo sapiens
Genetic Insert: Pax7
Vector Backbone Description: Vector Backbone:PRRL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29317646
Proper citation: RRID:Addgene_109372 Copy
Species: Homo sapiens
Genetic Insert: Gsi chimera
Vector Backbone Description: Backbone Size:6002; Vector Backbone:pEM705; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29338718
Proper citation: RRID:Addgene_109373 Copy
Species: Homo sapiens
Genetic Insert: HDR-Enhancer domain of human CtIP protein
Vector Backbone Description: Backbone Marker:Church lab A; Vector Backbone:hCas9 Addgene ID 41815; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29556040
Comments: Note from depositor: The sequence used for the HDR enhancer domain of CtIP has several differences compared to the reference human CtIP cDNA sequence including T176A. These differences were present in the CtIP expression vector used to make the constructs and do not affect activity.
Proper citation: RRID:Addgene_109400 Copy
Species: Homo sapiens
Genetic Insert: human Melanopsin-mGluR6 chimera
Vector Backbone Description: Backbone Size:5366; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29338718
Proper citation: RRID:Addgene_109368 Copy
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