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Species: Mus musculus
Genetic Insert: mNeonGreen-FLAG-mgp100-T2A-PuroR
Vector Backbone Description: Backbone Marker:Based on plasmids published by Schmid-Burgk et al. 2016 (DOI: 10.1038/ncomms12338); Backbone Size:2542; Vector Backbone:pCRISPaint; Vector Types:Gene tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32750334
Proper citation: RRID:Addgene_171802 Copy
Species: Branchiostoma lanceolatum, Streptomyces alboniger
Genetic Insert: mNeonGreen-T2A-PuroR
Vector Backbone Description: Backbone Marker:Based on plasmids published by Schmid-Burgk et al. 2016 (DOI: 10.1038/ncomms12338); Backbone Size:2516; Vector Backbone:pCRISPaint; Vector Types:Gene tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32750334
Proper citation: RRID:Addgene_171800 Copy
Species: Synthetic
Genetic Insert: mNeonGreen-F-Ova-T2A-BlastR
Vector Backbone Description: Backbone Marker:Based on plasmids published by Schmid-Burgk et al. 2016 (DOI: 10.1038/ncomms12338); Backbone Size:2577; Vector Backbone:pCRISPaint; Vector Types:Gene tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32750334
Proper citation: RRID:Addgene_171808 Copy
Species: Synthetic
Genetic Insert: mNeonGreen-FLAG-gB-T2A-PuroR
Vector Backbone Description: Backbone Marker:Based on plasmids published by Schmid-Burgk et al. 2016 (DOI: 10.1038/ncomms12338); Backbone Size:2542; Vector Backbone:pCRISPaint; Vector Types:Gene tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32750334
Proper citation: RRID:Addgene_171803 Copy
Species: Synthetic
Genetic Insert: mScarlet-F-gB-T2A-PuroR
Vector Backbone Description: Backbone Marker:Based on plasmids published by Schmid-Burgk et al. 2016 (DOI: 10.1038/ncomms12338); Backbone Size:2543; Vector Backbone:pCRISPaint; Vector Types:Gene tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32750334
Proper citation: RRID:Addgene_171812 Copy
Species: Mus musculus
Genetic Insert: mScarlet-F-mgp100-T2A-PuroR
Vector Backbone Description: Backbone Marker:Based on plasmids published by Schmid-Burgk et al. 2016 (DOI: 10.1038/ncomms12338); Backbone Size:2543; Vector Backbone:pCRISPaint; Vector Types:Gene tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32750334
Proper citation: RRID:Addgene_171811 Copy
Species: Homo sapiens
Genetic Insert: mScarlet-F-hgp100-T2A-PuroR
Vector Backbone Description: Backbone Marker:Based on plasmids published by Schmid-Burgk et al. 2016 (DOI: 10.1038/ncomms12338); Backbone Size:2543; Vector Backbone:pCRISPaint; Vector Types:Gene tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32750334
Proper citation: RRID:Addgene_171810 Copy
Species: Synthetic
Genetic Insert: mScarlet-F-Ova-T2A-BlastR
Vector Backbone Description: Backbone Marker:Based on plasmids published by Schmid-Burgk et al. 2016 (DOI: 10.1038/ncomms12338); Backbone Size:2577; Vector Backbone:pCRISPaint; Vector Types:Gene tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32750334
Proper citation: RRID:Addgene_171817 Copy
Species: Homo sapiens
Genetic Insert: mScarlet-F-hgp100-T2A-BlastR
Vector Backbone Description: Backbone Marker:Based on plasmids published by Schmid-Burgk et al. 2016 (DOI: 10.1038/ncomms12338); Backbone Size:2577; Vector Backbone:pCRISPaint; Vector Types:Gene tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32750334
Proper citation: RRID:Addgene_171815 Copy
Species: Discosoma sp., Bacillus cereus
Genetic Insert: mScarlet-T2A-BlastR
Vector Backbone Description: Backbone Marker:Based on plasmids published by Schmid-Burgk et al. 2016 (DOI: 10.1038/ncomms12338); Backbone Size:2577; Vector Backbone:pCRISPaint; Vector Types:Gene tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32750334
Proper citation: RRID:Addgene_171814 Copy
Species: Synthetic
Genetic Insert: DogCatcher
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:3641; Vector Backbone:pDEST14; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34324879
Proper citation: RRID:Addgene_171772 Copy
Species: Homo sapiens
Genetic Insert: TUBG2
Vector Backbone Description: Backbone Marker:thermofisher; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34158617
Comments: The internal EcoRI was removed from the GeneCopeia mCherry tagged TUBG2/pReceiver-M56 plasmid and TUBG2 was sub-cloned into pcDNA3.1.
Proper citation: RRID:Addgene_171966 Copy
Vector Backbone Description: Backbone Marker:Moon Lab; Backbone Size:0; Vector Backbone:pCMV pGL3 Luciferase; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Comments: Description: This is a home-cooked PGL3 Luciferase expression vector.
Reference: Ajamete Kaykas.
Proper citation: RRID:Addgene_17186 Copy
Species: Homo sapiens
Genetic Insert: beta catenin
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5400; Vector Backbone:pET-28 a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Description: This is an amino terminal HIS tag, TEV protease cleavage site, then human beta catenin domains R1C, subcloned in BamHI and Xho 1 sites.
Reference: Made by Ken-Ichi Takemaru.
Proper citation: RRID:Addgene_17200 Copy
Species: Synthetic
Genetic Insert: RNA-based (p)ppGpp sensor
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5267; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34487413
Comments: Addgene QC identified a C66Y change in the lacI coding sequence that may impact the leakiness of expression of the (p)ppGpp sensor and NirFP insert.
Proper citation: RRID:Addgene_171921 Copy
Species: Synthetic
Genetic Insert: CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
Vector Backbone Description: Vector Backbone:pCMV-PE2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34534334
Comments: Full sequence: https://benchling.com/s/seq-btUItL879sEOzAzXhUuy.
Proper citation: RRID:Addgene_171991 Copy
Species: Homo sapiens
Genetic Insert: Endophilin A1
Vector Backbone Description: Vector Backbone:mRuby2-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33947811
Proper citation: RRID:Addgene_171945 Copy
Species: Homo sapiens
Genetic Insert: beta catenin
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5400; Vector Backbone:pET-28 a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Description: This is full length human beta catenin cDNA with a TEV protease site at the amino terminus following the HIS tag. Cloned into BamH1 and Xho1 sites.
Reference: Made by Ken-Ichi Takemaru.
Proper citation: RRID:Addgene_17198 Copy
Species: Danio rerio
Genetic Insert: beta catenin
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5400; Vector Backbone:pET-28 b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Description: This is full length zebrafish beta catenin cDNA with a TEV protease site at the amino terminus following the HIS tag. Cloned into Sal1 and Not1 sites.
Reference: Made by Ken-Ichi Takemaru.
Proper citation: RRID:Addgene_17199 Copy
Species: Synthetic
Genetic Insert: DogCatcher-sfGFP
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pJ404; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34324879
Proper citation: RRID:Addgene_171930 Copy
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