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Species: Homo sapiens
Genetic Insert: Bmpr2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7350; Vector Backbone:pmirGLO; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24145352
Proper citation: RRID:Addgene_49380 Copy
Species: Tn10
Genetic Insert: TetR repressor
Vector Backbone Description: Vector Backbone:pEXT20; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24316737
Comments: Plasmid also contains YFP reporter (whose expression is controlled by a constitutive promoter containing an operator site for the indicated repressor).
Proper citation: RRID:Addgene_49374 Copy
Species: Other
Genetic Insert: AND gate
Vector Backbone Description: Vector Backbone:pEXT20; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24316737
Comments: AND gate controlled by Ptac and Ptet input promoters.
Proper citation: RRID:Addgene_49377 Copy
Species: Bacillus subtilis
Genetic Insert: LmrA repressor
Vector Backbone Description: Vector Backbone:pCI-YFP; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24316737
Comments: LmrA controlled by Plux promoter
Proper citation: RRID:Addgene_49375 Copy
Species: Drosophila melanogaster
Genetic Insert: dU6-2:gRNA
Vector Backbone Description: Backbone Marker:Norbert Perrimon, Jian-Quan Ni, Harvard; Vector Backbone:pValium22; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25002478
Comments: Please acknowledge Fillip Port and Simon Bullock when publishing work derived from use of this plasmid.
Visit crisprflydesign.org for more information.
Proper citation: RRID:Addgene_49409 Copy
Species: Homo sapiens
Genetic Insert: N Ras K61
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pMSCV-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_49404 Copy
Species: Mus musculus
Genetic Insert: 3prime end of Flag-mus-Ezh2deltaSET, eliminating Set domain
Vector Backbone Description: Vector Backbone:MSCV-hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: This was cloned using fusion polymerase but the sequence has not been confirmed.
Proper citation: RRID:Addgene_49403 Copy
Vector Backbone Description: Vector Backbone:pBluescript KS+; Vector Types:Insect Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24058473
Comments: Additional reference: The use of TALENs for nonhomologous end joining mutagenesis in silkworm and fruitfly. Takasu Y. et al. Methods 2014. PMID: 24565747
Proper citation: RRID:Addgene_49401 Copy
Species: Drosophila melanogaster
Genetic Insert: dU6-1:gRNA
Vector Backbone Description: Backbone Marker:Norbert Perrimon, Jian-Quan Ni, Harvard; Vector Backbone:pValium22; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25002478
Comments: Please acknowledge Fillip Port and Simon Bullock when publishing work derived from use of this plasmid.
Visit crisprflydesign.org for more information.
Proper citation: RRID:Addgene_49408 Copy
Species: Synthetic
Genetic Insert: luc2
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2693; Vector Backbone:pUC57; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25177895
Proper citation: RRID:Addgene_49352 Copy
Species: Synthetic
Genetic Insert: luc2
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2693; Vector Backbone:pUC57; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25177895
Proper citation: RRID:Addgene_49353 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4238; Vector Backbone:pGL4; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25177895
Proper citation: RRID:Addgene_49349 Copy
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Richard Mulligan; Backbone Size:4874; Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23403494
Proper citation: RRID:Addgene_49343 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Dr. James Sutherland ; Backbone Size:6600; Vector Backbone:pAc-STABLE1-Puro; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24326186
Comments: gRNA target sequence GGTTTTGGACACTGGAACCG
Proper citation: RRID:Addgene_49331 Copy
Species: Synthetic
Genetic Insert: GBP7-p65 transactivation domain fusion protein
Vector Backbone Description: Backbone Size:4823; Vector Backbone:pCAG-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23953120
Comments: Kirchhofer, A. et al. (2010). Modulation of protein properties in living cells using nanobodies. Nat. Struct. Mol. Biol. 17, 133–138.
Tang J.C. et al. (2013). A nanobody-based system using fluorescent proteins as scaffolds for cell-specific gene manipulation. Cell. 2013 Aug 15;154(4):928-39.
Proper citation: RRID:Addgene_49441 Copy
Species: Synthetic
Genetic Insert: GPD-roGFP2
Vector Backbone Description: Backbone Marker:ViraQuest Inc.; Backbone Size:5217; Vector Backbone:VQ Ad5CMV K-NpA; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20019331
Proper citation: RRID:Addgene_49436 Copy
Species: Synthetic
Genetic Insert: roGFP2
Vector Backbone Description: Backbone Marker:ViraQuest Inc.; Backbone Size:5217; Vector Backbone:VQ Ad5CMV K-NpA; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20019331
Proper citation: RRID:Addgene_49435 Copy
Species: Synthetic
Genetic Insert: GBP2-Gal4 DNA binding domain fusion protein
Vector Backbone Description: Backbone Size:4823; Vector Backbone:pCAG-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23953120
Comments: Kirchhofer, A. et al. (2010). Modulation of protein properties in living cells using nanobodies. Nat. Struct. Mol. Biol. 17, 133–138.
Tang J.C. et al. (2013). A nanobody-based system using fluorescent proteins as scaffolds for cell-specific gene manipulation. Cell. 2013 Aug 15;154(4):928-39.
Proper citation: RRID:Addgene_49439 Copy
Species: Synthetic
Genetic Insert: GBP1-Gal4 DNA binding domain fusion protein
Vector Backbone Description: Backbone Size:4823; Vector Backbone:pCAG-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23953120
Comments: The GBP1 used differs from the PDB GBP1 by two amino acids. One is Q3D at a site away from the GFP binding pocket and not expected to affect GFP binding. The second was a C98S mutation to enhance protein solubility.
Kirchhofer, A. et al. (2010). Modulation of protein properties in living cells using nanobodies. Nat. Struct. Mol. Biol. 17, 133–138.
Tang J.C. et al. (2013). A nanobody-based system using fluorescent proteins as scaffolds for cell-specific gene manipulation. Cell. 2013 Aug 15;154(4):928-39.
Proper citation: RRID:Addgene_49438 Copy
Species: Homo sapiens
Genetic Insert: Rab11A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:EGFP C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17908815
Proper citation: RRID:Addgene_49553 Copy
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