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Species: E. coli
Genetic Insert: TraM, TraJ
Vector Backbone Description: Backbone Marker:Registry of Standard Parts; Vector Backbone:pSB3C5; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_67966 Copy
Vector Backbone Description: Backbone Marker:epicentre; Vector Backbone:pCC1FOS; Vector Types:; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_68380 Copy
Species: Synthetic
Genetic Insert: S68TAG beta lactamase
Vector Backbone Description: Vector Backbone:pCRT7/NT-TOPO; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26350500
Comments: Used to assess phosphoserine or serine incorporation at TAG position corresponding to Ser68 in beta-lactamase protein. Ampicillin resistance only conferred if Ser is incorporated at this position.
Proper citation: RRID:Addgene_68305 Copy
Vector Backbone Description: Backbone Size:5743; Vector Backbone:N/A; Vector Types:Staphylococcal allelic exchange vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:22434850
Comments: It is prudent to check that the pIMAY is still temperature sensitive in Staph Aureus before starting any cloning. The author found out that it could lose this function while cultured in Ecoli. That's why Ecoli bearing pIMAY MUST be cultured at 37C in Chloramphenicol (10µg/ml).
Proper citation: RRID:Addgene_68939 Copy
Species: Synthetic
Genetic Insert: qacR
Vector Backbone Description: Backbone Marker:unknown; Backbone Size:1154; Vector Backbone:p15A Ori/cm resistant; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26262913
Proper citation: RRID:Addgene_69033 Copy
Species: Synthetic
Genetic Insert: qacR
Vector Backbone Description: Backbone Marker:unknown; Backbone Size:1154; Vector Backbone:p15A Ori/cm resistant; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26262913
Comments: Depositor notes that discrepancies between QC sequence and full plasmid sequence is not a concern for function.
Proper citation: RRID:Addgene_69031 Copy
Species: Synthetic
Genetic Insert: RNA-IN S3
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:2200; Vector Backbone:custom; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26350218
Comments: Mutated from sequence obtained from Arkin Lab (UC Berkeley)
Proper citation: RRID:Addgene_69455 Copy
Species: Synthetic
Genetic Insert: RNA-IN S4 with C24A, A25C mutations
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:2200; Vector Backbone:custom; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26350218
Comments: Mutated from sequence obtained from Arkin Lab (UC Berkeley)
Proper citation: RRID:Addgene_69463 Copy
Genetic Insert: GFPmut2
Vector Backbone Description: Vector Backbone:pBAD33t; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26283792
Proper citation: RRID:Addgene_69518 Copy
Genetic Insert: GFPmut2
Vector Backbone Description: Vector Backbone:pBAD33t; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26283792
Proper citation: RRID:Addgene_69515 Copy
Genetic Insert: GFPmut2
Vector Backbone Description: Vector Backbone:pBAD33t; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26283792
Proper citation: RRID:Addgene_69512 Copy
Genetic Insert: GFPmut2
Vector Backbone Description: Vector Backbone:pBAD33t; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26283792
Proper citation: RRID:Addgene_69521 Copy
Species: Other
Genetic Insert: dnaE74
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69629 Copy
Species: Other
Genetic Insert: dnaQ926 umuD' umuC recA730
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69627 Copy
Species: Other
Genetic Insert: dnaQ926 mutS538'
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69638 Copy
Species: Other
Genetic Insert: dnaQ926 dnaX2016
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69637 Copy
Species: Other
Genetic Insert: dnaQ926 dnaX36
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69636 Copy
Species: Other
Genetic Insert: dnaQ926 dnaE1026
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69635 Copy
Species: Other
Genetic Insert: rpsD16
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69649 Copy
Species: Other
Genetic Insert: rpsD14
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69648 Copy
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