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Species: Danio rerio
Genetic Insert: fishRab8A
Vector Backbone Description: Backbone Size:2500; Vector Backbone:pDONR; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17574030
Comments: This construct was generated by gene synthesis.
Proper citation: RRID:Addgene_24905 Copy
Species: Danio rerio
Genetic Insert: fishRab8A
Vector Backbone Description: Backbone Size:2500; Vector Backbone:pDONR; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17574030
Comments: This construct was generated by gene synthesis.
Proper citation: RRID:Addgene_24904 Copy
Species: Danio rerio
Genetic Insert: Papc
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript-SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9693142
Comments: antisense: linearize with Apa1, transcribe with T3
sense: linearize with Not1, transcribe with T7
Proper citation: RRID:Addgene_25774 Copy
Species: Danio rerio
Genetic Insert: ef1a
Vector Backbone Description: Backbone Size:0; Vector Backbone:pME; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20364122
Comments: EF1alpha gene insert (also known as ef1a, accession number NM_131263) encompassing the TATA box, first non-coding exon 1, intron 1 and partial exon 2, 5′ of the ATG.
There are minor mismatches in the ef1a region. These changes should not affect plasmid function.
Proper citation: RRID:Addgene_26032 Copy
Species: Danio rerio
Genetic Insert: Synaptophysin GCaMP2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3585; Vector Backbone:pBluescript II (KS); Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19898484
Comments: At both ends of the multiple cloning site of the original pBluescript II KS+, a I-SceI recognition site was cloned replacing the BssHII cutting sequence.
In the map, ORF frame 3 (1107-3368): zebrafish SyGCaMP2. Also, 5UAS is located 668-1034.
Proper citation: RRID:Addgene_26125 Copy
Species: Danio rerio
Genetic Insert: casanova
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:xenopus/mammalian/avian/zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11410530
Comments: For CG569/cas probe synthesis, NotI was used to linearize the plasmid and T7 RNA polymerase to generate antisense RNA.
Proper citation: RRID:Addgene_26729 Copy
Species: Danio rerio
Genetic Insert: Zinc finger array targeting cacna1f
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene CACNA1F (see below). Please note that this plasmid does NOT contain the CACNA1F sequence.
Users must order the complementary plasmid CACNA1F_R (OZ508) [Addgene plasmid 27183] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene.
Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203).
This zinc finger array was tested for binding activity to the sequence 5'-GAGGTCACG-3' in a bacterial two hybrid assay, and resulted in 3.89 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus).
Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication.
Other Articles:
"Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082
"Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934
Proper citation: RRID:Addgene_26957 Copy
Species: Danio rerio
Genetic Insert: Zinc finger array targeting PTRF/Cavin1
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene PTRF/Cavin1 (see below). Please note that this plasmid does NOT contain the PTRF/Cavin1 sequence.
Users must order the complementary plasmid PTRF/Cavin1_R (OZ514) [Addgene plasmid 27189] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene.
Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203).
This zinc finger array was tested for binding activity to the sequence 5'-GACGGCTGT-3' in a bacterial two hybrid assay, and resulted in 6.30 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus).
Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication.
Other Articles:
"Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082
"Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934
Proper citation: RRID:Addgene_27188 Copy
Species: Danio rerio
Genetic Insert: Zinc finger array targeting zgc:153086
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene zgc:153086 (see below). Please note that this plasmid does NOT contain the zgc:153086 sequence.
Users must order the complementary plasmid zgc:153086_R (OZ512) [Addgene plasmid 27187] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene.
Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203).
This zinc finger array was tested for binding activity to the sequence 5'-GGTGATGCT-3' in a bacterial two hybrid assay, and resulted in 11.90 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus).
Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication.
Other Articles:
"Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082
"Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934
Proper citation: RRID:Addgene_27186 Copy
Species: Danio rerio
Genetic Insert: Zinc finger array targeting xylt1
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene xylt1 (see below). Please note that this plasmid does NOT contain the xylt1 sequence.
Users must order the complementary plasmid xylt1_R (OZ508) [Addgene plasmid 27185] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene.
Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203).
This zinc finger array was tested for binding activity to the sequence 5'-GTAGAGGGT-3' in a bacterial two hybrid assay, and resulted in 6.48 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus).
Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication.
Other Articles:
"Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082
"Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934
Proper citation: RRID:Addgene_27184 Copy
Species: Danio rerio
Genetic Insert: Zinc finger array targeting xylt1
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene xylt1 (see below). Please note that this plasmid does NOT contain the xylt1 sequence.
Users must order the complementary plasmid xylt1_L (OZ509) [Addgene plasmid 27184] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene.
Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203).
This zinc finger array was tested for binding activity to the sequence 5'-GTAGCGGGT-3' in a bacterial two hybrid assay, and resulted in 5.09 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus).
Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication.
Other Articles:
"Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082
"Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934
Proper citation: RRID:Addgene_27185 Copy
Species: Danio rerio
Genetic Insert: nanos 3' UTR
Vector Backbone Description: Backbone Size:8272; Vector Backbone:pCS2-nCas9n; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26322839
Comments: vector backbone was Addgene Plasmid #47929 from Wenbiao Chen
Proper citation: RRID:Addgene_62542 Copy
Species: Danio rerio
Genetic Insert: zebrafish mmp21
Vector Backbone Description: Vector Backbone:pT7-gRNA; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26429889
Proper citation: RRID:Addgene_72890 Copy
Species: Danio rerio
Genetic Insert: jam2a
Vector Backbone Description: Backbone Marker:invitrogen; Vector Backbone:pCRII; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25119047
Proper citation: RRID:Addgene_68804 Copy
Species: Danio rerio
Genetic Insert: jam1a
Vector Backbone Description: Backbone Marker:invitrogen; Vector Backbone:pCRII; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25119047
Proper citation: RRID:Addgene_68803 Copy
Species: Danio rerio
Genetic Insert: beta-actin promoter
Vector Backbone Description: Vector Backbone:pExpress1; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20154733
Comments: Addgene NGS results found that plasmid contains 3x SV40pA repeats rather than the expected 4x as described in associated publication.
Proper citation: RRID:Addgene_68797 Copy
Species: Danio rerio
Genetic Insert: wnt16
Vector Backbone Description: Backbone Marker:invitrogen; Vector Backbone:pCRII; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21654806
Proper citation: RRID:Addgene_68796 Copy
Species: Danio rerio
Genetic Insert: shha
Vector Backbone Description: Vector Backbone:pCS2; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21654806
Proper citation: RRID:Addgene_68962 Copy
Species: Danio rerio
Genetic Insert: ZFCNGA5, 3' end only with native stop
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5362; Vector Backbone:pet28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19778592
Proper citation: RRID:Addgene_69858 Copy
Species: Danio rerio
Genetic Insert: SLC2A2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4500; Vector Backbone:pBK-CMV; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19776250
Proper citation: RRID:Addgene_65103 Copy
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