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Species: Saccharomyces cerevisiae
Genetic Insert: Ubiquitin - Leucine - 4MYC N-terminal tagging
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36650116
Proper citation: RRID:Addgene_193314 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SIR4 gRNA
Vector Backbone Description: Vector Backbone:pRS413; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36650116
Proper citation: RRID:Addgene_182520 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ubiquitin - Leucine - 4MYC N-terminal tagging
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36650116
Proper citation: RRID:Addgene_193316 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PI-SceI
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET-23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7890714
Comments: Expresses the excised intein of the VMA1 gene product including amino-terminal methionine-cysteine residues. Contains silent AvrII and RsrII sites near the borders of the N-terminal LAGLIDADG motif and silent EaeI and SnaBI sites near the borders of the C-terminal motif.
Proper citation: RRID:Addgene_195607 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: I-SceI
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18644379
Comments: Expresses the I-SceI homing endonuclease under the control of the phage T7 promoter. The sequence is codon optimized for expression in E. coli and is derived from plasmid pSCM525 (Dr. Bernard Dujon, Institut Pasteur). The protein contains a carboxyl-terminal histidine tag for purification. The expressed protein contains the K223I mutation that results in DNA nicking activity. An appended methionine-histidine sequence is present at the amino terminus.
Proper citation: RRID:Addgene_195615 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PI-SceI
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET-23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9468518
Comments: Expresses the excised intein of the VMA1 gene product including amino-terminal methionine-cysteine residues. Contains silent AvrII and RsrII sites near the borders of the N-terminal LAGLIDADG motif and silent EaeI and SnaBI sites near the borders of the C-terminal motif. Also contains silent mutations that introduce ApaI, BssHII, BstEII, BsiWI, MluI and SpeI sites. Expresses PI-SceI containing a carboxyl-terminal histidine tag and a K301A mutation in the PI-SceI ORF.
Proper citation: RRID:Addgene_195611 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PI-SceI
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET-23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7890714
Comments: Expresses the excised intein of the VMA1 gene product including amino-terminal methionine-cysteine residues. Contains silent AvrII and RsrII sites near the borders of the N-terminal LAGLIDADG motif and silent EaeI and SnaBI sites near the borders of the C-terminal motif. Contains a D218A mutation in the first LAGLIDADG motif that inactivates the protein.
Proper citation: RRID:Addgene_195608 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PI-SceI
Vector Backbone Description: Vector Backbone:pT7-7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7890714
Comments: Expresses the excised intein of the VMA1 gene product including amino-terminal methionine-cysteine residues. Contains silent AvrII and RsrII sites near the borders of the N-terminal LAGLIDADG motif and silent EaeI and SnaBI sites near the borders of the C-terminal motif. Contains a D326A mutation in the second LAGLIDADG motif that inactivates the protein.
Proper citation: RRID:Addgene_195609 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CEN6/ARSH4 ORI
Vector Backbone Description: Vector Backbone:pGLDV_17; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_198928 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 2μ ORI
Vector Backbone Description: Vector Backbone:pGLDV_17; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_198929 Copy
Species: Saccharomyces Cerevisiae
Genetic Insert: Atg32
Vector Backbone Description: Vector Backbone:pET His6 TEV LIC cloning vector (1B); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29909755
Proper citation: RRID:Addgene_190431 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pFA6-yomRuby3-SpHIS5
Vector Backbone Description: Backbone Size:4021; Vector Backbone:pFA-Envy-SpHIS5 (Addgene plasmid 60782); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37436806
Proper citation: RRID:Addgene_204287 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tRNA-gRNA scaffold
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:3500; Vector Backbone:pCR-Blunt II-TOPO; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37326023
Comments: A common PCR template used fo CRISPR Engineering of EPisomes in Yeast, or CREEPY, a method for rapid engineering of large synthetic episomal DNA constructs. This is designed and assembled based on the template consisting of gRNA scaffold with a tRNA sequence, as published in Zhang, Yueping, et al. "A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae." Nature communications 10.1 (2019): 1053.
Proper citation: RRID:Addgene_200227 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cas9 (codon optimized for human)
Vector Backbone Description: Backbone Size:4898; Vector Backbone:pRS416; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37326023
Proper citation: RRID:Addgene_192603 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cas9 (codon optimized for human)
Vector Backbone Description: Backbone Size:4898; Vector Backbone:pRS416; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37326023
Proper citation: RRID:Addgene_187970 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VP2C-FPPS(G)-NES
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_182476 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VP2C-FPPS
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_182433 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VP2C-FPPS-NES
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_182432 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VP2C-FPPS(M)
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_182435 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VP2C-FPPS(M)-NES
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_182434 Copy
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