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Species: Mus musculus
Genetic Insert: PI3K regulatory subunit p55alpha
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pCMV6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Cantley lab recommends growth at room temperature.
Proper citation: RRID:Addgene_1402 Copy
Species: Mus musculus
Genetic Insert: Tcf4B
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14667415
Proper citation: RRID:Addgene_14019 Copy
Species: Mus musculus
Genetic Insert: Wnt1
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Comments: Contains an internal Cla I site, from Javier Capdevila.
Proper citation: RRID:Addgene_14017 Copy
Species: Mus musculus
Genetic Insert: Csrp2
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:pDTA-4; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: Linker between sfGFP and iCre-ERT2 is a mutated P2A sequence where 2 conserved amino acids are changed to Alanines. This mutated sequence does not skip.
The sfGFP fluorescence from the sfGFP-iCre-ERT2 is dim, as described in Nam and Capecchi, 2020 (https://doi.org/10.1186/s13064-020-00139-5) and a Figshare page (https://doi.org/10.6084/m9.figshare.13218437.v35). Thus, the sfGFP fluorescence should be detectable only in cells in which the marker gene expression level is high.
Homology arms subcloned by recombineering from a clone of RPCI-24 C57BL/6J mouse genomic DNA library.
Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.
Please note there are a few ambiguous bases in Addgene’s NGS result. This is due to repetitive T-A regions. Depositor confirms these bases do not affect plasmid function.
Proper citation: RRID:Addgene_140270 Copy
Species: Mus musculus
Genetic Insert: XAF1 sgRNA
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_140290 Copy
Species: Mus musculus
Genetic Insert: Lmx1b
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: EcoRI fragment containing full length mouse Lmx-1 cDNA, from Randy Johnson.
Proper citation: RRID:Addgene_14025 Copy
Species: Mus musculus
Genetic Insert: Lef1
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Comments: From Gabrielle Kardon.
Proper citation: RRID:Addgene_14021 Copy
Species: Mus musculus
Genetic Insert: Gfap
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:pDTA-5; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin
Comments: A targeting vector to knock-in T2A-td-sfGFP into mouse Gfap protein isoform 2 (also known as Gfap-alpha). This is the most abundant isoform in the mouse brain. Of note, the Gfap protein transcript variant 2 transcript level is high in the SVZ, an adult neural stem cell niche. See Kamphuis et al., 2012 (https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0042823).
Homology arms subcloned from a clone of RPCI-23 C57BL/6J mouse genomic DNA library.
Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.
Proper citation: RRID:Addgene_137044 Copy
Species: Mus musculus
Genetic Insert: Acetyl CoA Synthetase 1
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:4700; Vector Backbone:pQE80; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16790548
Proper citation: RRID:Addgene_13746 Copy
Species: Mus musculus
Genetic Insert: mouse Kif3A
Vector Backbone Description: Backbone Size:5058; Vector Backbone:pGREENLANTERN-1; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11102514
Proper citation: RRID:Addgene_13742 Copy
Species: Mus musculus
Genetic Insert: Truncated Acetyl CoA Sythetase 2
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:4700; Vector Backbone:pQE80; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16790548
Proper citation: RRID:Addgene_13748 Copy
Species: Mus musculus
Genetic Insert: Crx promoter
Vector Backbone Description: Backbone Size:3078; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17209010
Comments: The mouse Crx promoter is active in photoreceptor and bipolar cells in the retina.
Proper citation: RRID:Addgene_13765 Copy
Species: Mus musculus
Genetic Insert: TKR2/4 microRNA
Vector Backbone Description: Backbone Marker:Åkerblom et al., 2013; Vector Backbone:pLV-PGK-GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30033154
Comments: the website for the BLOCK-iT RNAi Designer
Proper citation: RRID:Addgene_137733 Copy
Species: Mus musculus
Genetic Insert: mouse Lin28A, CSD and ZnFs (residues 36–186)
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26045559
Proper citation: RRID:Addgene_137998 Copy
Species: Mus musculus
Genetic Insert: SA-EGFP-bpA with micro-homology arms
Vector Backbone Description: Backbone Size:2955; Vector Backbone:pBluescriptII SK+; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32433962
Proper citation: RRID:Addgene_137926 Copy
Species: Mus musculus
Genetic Insert: Cerulean-Epac2(R53A)-Venus
Vector Backbone Description: Backbone Size:5380; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21338921
Proper citation: RRID:Addgene_138197 Copy
Species: Mus musculus
Genetic Insert: Cerulean-Epac2(R466A)-Venus
Vector Backbone Description: Backbone Size:5380; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21338921
Proper citation: RRID:Addgene_138199 Copy
Species: Mus musculus
Genetic Insert: Cerulean-Epac2(L426W)-Venus
Vector Backbone Description: Backbone Size:5380; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21338921
Proper citation: RRID:Addgene_138200 Copy
Species: Mus musculus
Genetic Insert: ERas CVLS
Vector Backbone Description: Backbone Size:6900; Vector Backbone:pMX-EGFP-gw-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16046393
Proper citation: RRID:Addgene_13828 Copy
Species: Mus musculus
Genetic Insert: ERas
Vector Backbone Description: Backbone Size:6900; Vector Backbone:pMX-EGFP-gw-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16046393
Proper citation: RRID:Addgene_13826 Copy
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