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  • Organism:saccharomyces cerevisiae (facet)

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NAT-TET-OFF – ubiquitin – LEU – 4MYC
 
Resource Report
Resource Website 		RRID:Addgene_193314 Ubiquitin - Leucine - 4MYC N-terminal tagging Saccharomyces cerevisiae Ampicillin PMID:36650116 Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:25:01 0
SIR4 gRNA HIS
 
Resource Report
Resource Website 		RRID:Addgene_182520 SIR4 gRNA Saccharomyces cerevisiae Ampicillin PMID:36650116 Vector Backbone:pRS413; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:25:00 0
HYG-TET-OFF – ubiquitin – LEU – 4MYC
 
Resource Report
Resource Website 		RRID:Addgene_193316 Ubiquitin - Leucine - 4MYC N-terminal tagging Saccharomyces cerevisiae Ampicillin PMID:36650116 Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:25:01 0
pET23PI-Sce ESARC
 
Resource Report
Resource Website 		RRID:Addgene_195607 PI-SceI Saccharomyces cerevisiae Ampicillin PMID:7890714 Expresses the excised intein of the VMA1 gene product including amino-terminal methionine-cysteine residues. Contains silent AvrII and RsrII sites near the borders of the N-terminal LAGLIDADG motif and silent EaeI and SnaBI sites near the borders of the C-terminal motif. Backbone Marker:Novagen; Vector Backbone:pET-23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin C552T,C554A, A557T,G584T, C588A, G878T, C881T, C905T, A908T 2026-08-15 01:25:05 0
pET15b I-SceI (Ctermhis) K223I
 
Resource Report
Resource Website 		RRID:Addgene_195615 I-SceI Saccharomyces cerevisiae Ampicillin PMID:18644379 Expresses the I-SceI homing endonuclease under the control of the phage T7 promoter. The sequence is codon optimized for expression in E. coli and is derived from plasmid pSCM525 (Dr. Bernard Dujon, Institut Pasteur). The protein contains a carboxyl-terminal histidine tag for purification. The expressed protein contains the K223I mutation that results in DNA nicking activity. An appended methionine-histidine sequence is present at the amino terminus. Backbone Marker:Novagen; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin C2694G, G2345C, G2351C, A3365T, A3366T 2026-08-15 01:25:03 0
pET PI-SceI C-His K301A
 
Resource Report
Resource Website 		RRID:Addgene_195611 PI-SceI Saccharomyces cerevisiae Ampicillin PMID:9468518 Expresses the excised intein of the VMA1 gene product including amino-terminal methionine-cysteine residues. Contains silent AvrII and RsrII sites near the borders of the N-terminal LAGLIDADG motif and silent EaeI and SnaBI sites near the borders of the C-terminal motif. Also contains silent mutations that introduce ApaI, BssHII, BstEII, BsiWI, MluI and SpeI sites. Expresses PI-SceI containing a carboxyl-terminal histidine tag and a K301A mutation in the PI-SceI ORF. Backbone Marker:Novagen; Vector Backbone:pET-23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin A822G, A823C, A824T 2026-08-15 01:25:03 0
pET23PI-Sce ESARC D218A
 
Resource Report
Resource Website 		RRID:Addgene_195608 PI-SceI Saccharomyces cerevisiae Ampicillin PMID:7890714 Expresses the excised intein of the VMA1 gene product including amino-terminal methionine-cysteine residues. Contains silent AvrII and RsrII sites near the borders of the N-terminal LAGLIDADG motif and silent EaeI and SnaBI sites near the borders of the C-terminal motif. Contains a D218A mutation in the first LAGLIDADG motif that inactivates the protein. Backbone Marker:Novagen; Vector Backbone:pET-23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin T928C, T929C, A942C 2026-08-15 01:25:06 0
pT7PI-Sce ESARC D326A
 
Resource Report
Resource Website 		RRID:Addgene_195609 PI-SceI Saccharomyces cerevisiae Ampicillin PMID:7890714 Expresses the excised intein of the VMA1 gene product including amino-terminal methionine-cysteine residues. Contains silent AvrII and RsrII sites near the borders of the N-terminal LAGLIDADG motif and silent EaeI and SnaBI sites near the borders of the C-terminal motif. Contains a D326A mutation in the second LAGLIDADG motif that inactivates the protein. Vector Backbone:pT7-7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin T3593C, A3601C, T3602G 2026-08-15 01:25:03 0
pGL0_15 [CEN6/ARSH4 ORI]
 
Resource Report
Resource Website 		RRID:Addgene_198928 CEN6/ARSH4 ORI Saccharomyces cerevisiae Chloramphenicol Vector Backbone:pGLDV_17; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:27:13 0
pGL0_16 [2μ ORI]
 
Resource Report
Resource Website 		RRID:Addgene_198929 2μ ORI Saccharomyces cerevisiae Chloramphenicol Vector Backbone:pGLDV_17; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:27:13 0
Atg32 [200-341] 1B
 
Resource Report
Resource Website 		RRID:Addgene_190431 Atg32 Saccharomyces Cerevisiae Kanamycin PMID:29909755 Vector Backbone:pET His6 TEV LIC cloning vector (1B); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:27:14 0
pFA6a-yomRuby3-SpHIS5
 
Resource Report
Resource Website 		RRID:Addgene_204287 pFA6-yomRuby3-SpHIS5 Saccharomyces cerevisiae Ampicillin PMID:37436806 Backbone Size:4021; Vector Backbone:pFA-Envy-SpHIS5 (Addgene plasmid 60782); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:27:15 0
pYZ038
 
Resource Report
Resource Website 		RRID:Addgene_200227 tRNA-gRNA scaffold Saccharomyces cerevisiae Kanamycin PMID:37326023 A common PCR template used fo CRISPR Engineering of EPisomes in Yeast, or CREEPY, a method for rapid engineering of large synthetic episomal DNA constructs. This is designed and assembled based on the template consisting of gRNA scaffold with a tRNA sequence, as published in Zhang, Yueping, et al. "A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae." Nature communications 10.1 (2019): 1053. Backbone Marker:Thermo Fisher; Backbone Size:3500; Vector Backbone:pCR-Blunt II-TOPO; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:27:24 0
pYZ959
 
Resource Report
Resource Website 		RRID:Addgene_192603 Cas9 (codon optimized for human) Saccharomyces cerevisiae Ampicillin PMID:37326023 Backbone Size:4898; Vector Backbone:pRS416; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:27:22 0
pYZ462
 
Resource Report
Resource Website 		RRID:Addgene_187970 Cas9 (codon optimized for human) Saccharomyces cerevisiae Ampicillin PMID:37326023 Backbone Size:4898; Vector Backbone:pRS416; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:27:25 0
Linked VLP NES (G)
 
Resource Report
Resource Website 		RRID:Addgene_182476 VP2C-FPPS(G)-NES Saccharomyces cerevisiae Ampicillin Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint. Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin 2026-08-15 01:24:28 0
Coexpressed VLP NES
 
Resource Report
Resource Website 		RRID:Addgene_182433 VP2C-FPPS Saccharomyces cerevisiae Ampicillin Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint. Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin 2026-08-15 01:24:28 0
Linked VLP NES
 
Resource Report
Resource Website 		RRID:Addgene_182432 VP2C-FPPS-NES Saccharomyces cerevisiae Ampicillin Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint. Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin 2026-08-15 01:24:28 0
Coexpressed VLP NES (M)
 
Resource Report
Resource Website 		RRID:Addgene_182435 VP2C-FPPS(M) Saccharomyces cerevisiae Ampicillin Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint. Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin 2026-08-15 01:24:28 0
Linked VLP NES (M)
 
Resource Report
Resource Website 		RRID:Addgene_182434 VP2C-FPPS(M)-NES Saccharomyces cerevisiae Ampicillin Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint. Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin 2026-08-15 01:24:28 0

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