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Species: Saccharomyces cerevisiae
Genetic Insert: sp_P32603_SPR1_YEAST:0-20
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:39674781
Comments: Signal peptides direct recombinant protein secretion in yeast. Codon optimised for expression in S. cerevisiae. This part is designed for use with components from the MoClo Yeast Toolkit (Lee et al. 2015). This insert is a 3a* component, see Williams et al. 2024 for more detail.
Proper citation: RRID:Addgene_228187 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: sp_Q05164_HPF1_YEAST:0-23
Vector Backbone Description: Backbone Marker:John Dueber, Addgene plasmid #65108; Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:39674781
Comments: Signal peptides direct recombinant protein secretion in yeast. Codon optimised for expression in S. cerevisiae. This part is designed for use with components from the MoClo Yeast Toolkit (Lee et al. 2015). This insert is a 3a* component, see Williams et al. 2024 for more detail.
Proper citation: RRID:Addgene_228188 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VIP1
Vector Backbone Description: Vector Backbone:pBB3; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:39966396
Proper citation: RRID:Addgene_238311 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VIP1
Vector Backbone Description: Vector Backbone:pBB3; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:39966396
Proper citation: RRID:Addgene_238317 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VIP1
Vector Backbone Description: Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:39966396
Proper citation: RRID:Addgene_238316 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ScYea4p-mTurquoise2-2xCrk_SH3
Vector Backbone Description: Backbone Marker:Internal; Vector Backbone:TMP138; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Comments: Additional inserts: 1) ScYnd1p(1-495)-ePTS1; 2) ScPex22(1-36)-mRuby2-SH3pep_dead
Proper citation: RRID:Addgene_239514 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 3V46
Vector Backbone Description: Backbone Size:5679; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42555168
Comments: 5' cloning site: BsaI (destroyed), 3' cloning site: BsaI (destroyed)
Proper citation: RRID:Addgene_234165 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 3ONH
Vector Backbone Description: Backbone Size:5679; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42555168
Comments: 5' cloning site: BsaI (destroyed), 3' cloning site: BsaI (destroyed)
Proper citation: RRID:Addgene_234179 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MFA-IgKappa-FAPoptim-STE3
Vector Backbone Description: Vector Backbone:pRS415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38809589
Comments: The pRS415-TEF1pr-MFA-NFAPoptim plasmid was used to insert the Ste3 coding sequence as an in-frame N-terminal fusion to FAP. The Ste3 coding sequence (lacking the stop codon) was inserted into the SmaI and XhoI restriction enzyme sites. The resulting plasmid contains the MFA1 ER targeting and secretion sequence, an N-terminal FAPoptim tag on Ste3.
Proper citation: RRID:Addgene_221114 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MFA-IgKappa-FAPoptim-STE3-K424R
Vector Backbone Description: Vector Backbone:pRS415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38809589
Comments: The lysine at position 242 in Ste3s C-tail was mutated to arginine using site-directed mutagenesis of pRS415-STE3pr-MFA-Igkappa-FAP-STE3.
Proper citation: RRID:Addgene_221119 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ERG6-FAPoptim
Vector Backbone Description: Vector Backbone:pRS413; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38809589
Comments: The coding sequence of ERG6 lacking the stop codon was PCR amplified with primers containing BamHI and SmaI restriction enzyme adaptors. The PCR product was then inserted into the pRS413-TEF1pr-NFAPoptim plasmid using standard cloning approaches.
Proper citation: RRID:Addgene_221121 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pHluorin
Vector Backbone Description: Vector Backbone:pFA6-HPHMX4; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38809589
Comments: This pHluorin integration plasmid was constructed by subcloning the 977 bp SalI/BglII fragment from pBW1571 (pFA6a-pHluorin::KANMX6 from (Prosser et al., 2010) into the SalI/BglII sites of pAG32 (pFA6-HPHMX4 described in (Goldstein and McCusker, 1999). The resulting plasmid was used for C-terminal tagging of the endogenous STE3 locus using a PCR-based integration strategy described in (Longtine et al., 1998)
Proper citation: RRID:Addgene_221127 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPA34-FAPoptim
Vector Backbone Description: Vector Backbone:pRS413; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38809589
Comments: The coding sequence of RPA34 lacking the stop codon was PCR amplified with primers containing BamHI and SmaI restriction enzyme adaptors. The PCR product was then inserted into the pRS413-TEF1pr-NFAPoptim plasmid using standard cloning approaches.
Proper citation: RRID:Addgene_221123 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PMA1-FAPoptim
Vector Backbone Description: Vector Backbone:pRS413; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38809589
Comments: The coding sequence of PMA1 lacking the stop codon was PCR amplified with primers containing BamHI and SmaI restriction enzyme adaptors. The PCR product was then inserted into the pRS413-TEF1pr-NFAPoptim plasmid using standard cloning approaches.
Proper citation: RRID:Addgene_221122 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VPH1-FAPoptim
Vector Backbone Description: Vector Backbone:pRS413; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38809589
Comments: The coding sequence of VPH1 lacking the stop codon was PCR amplified with primers containing BamHI and SmaI restriction enzyme adaptors. The PCR product was then inserted into the pRS413-TEF1pr-NFAPoptim plasmid using standard cloning approaches.
Proper citation: RRID:Addgene_221125 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SEC7-FAPoptim
Vector Backbone Description: Vector Backbone:pRS413; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38809589
Comments: The coding sequence of SEC7 lacking the stop codon was PCR amplified with primers containing BamHI and SmaI restriction enzyme adaptors. The PCR product was then inserted into the pRS413-TEF1pr-NFAPoptim plasmid using standard cloning approaches.
Proper citation: RRID:Addgene_221124 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 solvvol W
Vector Backbone Description: Backbone Marker:Invtirogen; Vector Backbone:pDest17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: The depositing lab recommends using BL21_pLysS strain for protein expression and purification. Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_231878 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 P+
Vector Backbone Description: Backbone Marker:Invtirogen; Vector Backbone:pDest17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: The depositing lab recommends using BL21_pLysS strain for protein expression and purification. Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_231872 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 R3
Vector Backbone Description: Backbone Marker:Invtirogen; Vector Backbone:pDest17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: The depositing lab recommends using BL21_pLysS strain for protein expression and purification. Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_231874 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gcn4 L113A+
Vector Backbone Description: Backbone Marker:Invtirogen; Vector Backbone:pDest17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39605529
Comments: The depositing lab recommends using BL21_pLysS strain for protein expression and purification. Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.
Proper citation: RRID:Addgene_231873 Copy
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