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Species: Synthetic
Genetic Insert: SARS-COV-2 Nsp7
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34154407
Proper citation: RRID:Addgene_166860 Copy
Species: Synthetic
Genetic Insert: Phi29 Polymerase codon-optimized for overexpression in E. coli
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pGS-21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21071419
Proper citation: RRID:Addgene_166863 Copy
Species: Synthetic
Genetic Insert: non-targeting presgRNA
Vector Backbone Description: Backbone Marker:Plasmid #109054; Backbone Size:2927; Vector Backbone:pXR004: CasRx pre-gRNA cloning backbone; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34178991
Proper citation: RRID:Addgene_166867 Copy
Species: Synthetic
Genetic Insert: Toehold Switch - Block G - Flanked by BsaI sites
Vector Backbone Description: Backbone Marker:GeneArt - Thermo Fisher; Backbone Size:2456; Vector Backbone:pMA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33741937
Proper citation: RRID:Addgene_166932 Copy
Species: Synthetic
Genetic Insert: Green Fluorescent Protein - Block F- Flanked by BsaI sites
Vector Backbone Description: Backbone Marker:Twist Bioscience; Backbone Size:2221; Vector Backbone:pTwist Amp High Copy; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33741937
Proper citation: RRID:Addgene_166931 Copy
Species: Synthetic
Genetic Insert: STOP CODON TAA and spacer - BLOCK E- Flanked by BsaI sites
Vector Backbone Description: Backbone Marker:GeneArt - Thermo Fisher; Backbone Size:2456; Vector Backbone:pMA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33741937
Proper citation: RRID:Addgene_166930 Copy
Species: Synthetic
Genetic Insert: PTAC promoter - Block H- Flanked by BsaI restriction sites.
Vector Backbone Description: Backbone Marker:GeneArt; Backbone Size:2456; Vector Backbone:pMA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33741937
Proper citation: RRID:Addgene_166936 Copy
Species: Synthetic
Genetic Insert: pT181 dual control activator (DC) - Flanked by BsaI restriction sites
Vector Backbone Description: Backbone Marker:GeneArt - Thermo Fisher; Backbone Size:2456; Vector Backbone:pMA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33741937
Proper citation: RRID:Addgene_166935 Copy
Species: Synthetic
Genetic Insert: Star sense AD1.S6 - Block G- Flanked by BsaI sites
Vector Backbone Description: Backbone Marker:GeneArt - Thermo Fisher; Backbone Size:2456; Vector Backbone:pMA; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33741937
Proper citation: RRID:Addgene_166934 Copy
Species: Synthetic
Genetic Insert: sRNA ToeHold - Block C- Flanked by BsaI sites
Vector Backbone Description: Backbone Size:2456; Vector Backbone:pMA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33741937
Proper citation: RRID:Addgene_166925 Copy
Species: Synthetic
Genetic Insert: PTAC promoter - Block B- Flanked by BsaI restriction sites.
Vector Backbone Description: Backbone Marker:GeneArt - Thermo Fisher; Backbone Size:2456; Vector Backbone:pMA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33741937
Proper citation: RRID:Addgene_166924 Copy
Species: Synthetic
Genetic Insert: Star Antisense AD1.A5- Block C- Flanked by BsaI restriction sites
Vector Backbone Description: Backbone Marker:GeneArt - Thermo Fisher; Backbone Size:2456; Vector Backbone:pMA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33741937
Proper citation: RRID:Addgene_166926 Copy
Species: Synthetic
Genetic Insert: Circuit expressing GFP- Small transcription activating RNAs
Vector Backbone Description: Backbone Size:1704; Vector Backbone:pBR322; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33741937
Proper citation: RRID:Addgene_166940 Copy
Species: Synthetic
Genetic Insert: Termination module:PolyU(0)
Vector Backbone Description: Vector Backbone:pAV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33811918
Proper citation: RRID:Addgene_166994 Copy
Species: Synthetic
Genetic Insert: Termination module:PolyU(0)
Vector Backbone Description: Vector Backbone:pAV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33811918
Proper citation: RRID:Addgene_166992 Copy
Species: Synthetic
Genetic Insert: Termination module:PolyU(4), Hairpin = 0 nt
Vector Backbone Description: Vector Backbone:pAV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33811918
Proper citation: RRID:Addgene_166991 Copy
Species: Synthetic
Genetic Insert: Negative control sgRNA 1
Vector Backbone Description: Backbone Marker:Addgene #99375; Backbone Size:9524; Vector Backbone:LentiGuide_Hygro-eGFP; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33112375
Proper citation: RRID:Addgene_166998 Copy
Species: Synthetic
Genetic Insert: Lactate oxidase
Vector Backbone Description: Backbone Size:12266; Vector Backbone:pTYF; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33400321
Proper citation: RRID:Addgene_166919 Copy
Species: Synthetic
Genetic Insert: EKARrEV-NLS
Vector Backbone Description: Backbone Size:6600; Vector Backbone:pPBbsr2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34667080
Comments: Please note there are several ambiguous bases in EKARrEV. This is a highly repetitive region. Depositor is not concerned and ensures this plasmid is functional.
Proper citation: RRID:Addgene_173855 Copy
Species: Synthetic
Genetic Insert: mRFP-FKBP-mSos1-linkercat
Vector Backbone Description: Backbone Size:6700; Vector Backbone:pT2Aneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34667080
Proper citation: RRID:Addgene_173858 Copy
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