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Species: Caenorhabditis elegans
Genetic Insert: GFP::mlcd-1
Vector Backbone Description: Backbone Size:3616; Vector Backbone:pRSET B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41773048
Comments: Please visit for https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 bioRxiv preprint.
There is a single nt discrepancy in Pgpc-1. This does not affect the plasmid's function.
Proper citation: RRID:Addgene_253863 Copy
Species: Caenorhabditis elegans
Genetic Insert: GFP::C54F6.5 3'UTR
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41773048
Comments: Please visit for https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 bioRxiv preprint.
There is a single nt discrepancy in Pgpc-1. This does not affect the plasmid's function.
The EGFP in the plasmid has synthetic introns in it to help expression in C. elegans. The mutations or indels are all in the introns and does not affect the protein expression or function. We observed good GFP expression from the plasmid
Proper citation: RRID:Addgene_253862 Copy
Species: Caenorhabditis elegans
Genetic Insert: SL2 NLS mNeptune
Vector Backbone Description: Backbone Size:5900; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41773048
Comments: Please visit for https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 bioRxiv preprint.
There is a single nt discrepancy in Pgpc-1. This does not affect the plasmid's function.
The Addgene sequence result finds a 1bp indel at the 3' end of the ORF containing mCardinal that causes a premature stop codon. The indel does not affect the expression and localization of the NLS::mNeptune. The protein in the plasmid expresses a few amino acids past the final Lysine of mNeptune and therefore, the premature stop does not truncate any functional part of the fluorescent protein. We observed bright nuclear RFP signal with this plasmid.
Proper citation: RRID:Addgene_253948 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmyo-2::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTR
Vector Backbone Description: Backbone Marker:Erik Jorgensen, Addgene plasmid #73716; Vector Backbone:pMLS257; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39841730
Proper citation: RRID:Addgene_229879 Copy
Species: Caenorhabditis elegans
Genetic Insert: Psnt-1::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR
Vector Backbone Description: Backbone Marker:Erik Jorgensen, Addgene plasmid #73716; Vector Backbone:pMLS257; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39841730
Proper citation: RRID:Addgene_229878 Copy
Species: Caenorhabditis elegans
Genetic Insert: cGAL[cGAL(DBD)-linker-QFAD]-tbb-2 3'UTR::SEC
Vector Backbone Description: Vector Backbone:pHW1133; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39841730
Proper citation: RRID:Addgene_229870 Copy
Species: Caenorhabditis elegans
Genetic Insert: QF-act-4 3' UTR::SEC
Vector Backbone Description: Vector Backbone:pHW1133; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39841730
Proper citation: RRID:Addgene_229873 Copy
Species: Caenorhabditis elegans
Genetic Insert: Tet-Off[TetR-QFAD]-act-4 3'UTR::SEC
Vector Backbone Description: Vector Backbone:pHW1133; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39841730
Proper citation: RRID:Addgene_229872 Copy
Species: Caenorhabditis elegans
Genetic Insert: QF2[QF(DBD)-linker-QFAD]-act-4 3'UTR::SEC
Vector Backbone Description: Vector Backbone:pHW1133; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39841730
Proper citation: RRID:Addgene_229874 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmyo-2::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR
Vector Backbone Description: Backbone Marker:Erik Jorgensen, Addgene plasmid #73716; Vector Backbone:pMLS257; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39841730
Proper citation: RRID:Addgene_229880 Copy
Species: Caenorhabditis elegans
Genetic Insert: FRT-SA-ArtificialExon-3xStopCodons-SL2-AAAA
Vector Backbone Description: Vector Backbone:pNM3468; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39841730
Proper citation: RRID:Addgene_229866 Copy
Species: Caenorhabditis elegans
Genetic Insert: PRDX-2
Vector Backbone Description: Backbone Marker:Anuj Sharma; Vector Backbone:pAS3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38733622
Proper citation: RRID:Addgene_232609 Copy
Species: Caenorhabditis elegans
Genetic Insert: GUR-3
Vector Backbone Description: Backbone Marker:Anuj Sharma; Vector Backbone:pAS3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38733622
Proper citation: RRID:Addgene_232605 Copy
Species: Caenorhabditis elegans
Genetic Insert: GUR-3
Vector Backbone Description: Backbone Marker:Anuj Sharma; Vector Backbone:pAS3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38733622
Proper citation: RRID:Addgene_232608 Copy
Species: Caenorhabditis elegans
Genetic Insert: LITE-1
Vector Backbone Description: Backbone Marker:Anuj Sharma; Vector Backbone:pAS3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38733622
Proper citation: RRID:Addgene_232610 Copy
Species: Caenorhabditis elegans
Genetic Insert: GUR-3
Vector Backbone Description: Backbone Marker:Anuj Sharma; Vector Backbone:pAS3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38733622
Proper citation: RRID:Addgene_232613 Copy
Species: Caenorhabditis elegans
Genetic Insert: pSKI
Vector Backbone Description: Backbone Size:2598; Vector Backbone:pPD95_75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40973646
Comments: Please visit https://doi.org/10.1101/2024.12.06.627295 for bioRxiv preprint.
Proper citation: RRID:Addgene_232484 Copy
Species: Caenorhabditis elegans
Genetic Insert: TUB1
Vector Backbone Description: Vector Backbone:pcDNA 3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39695235
Proper citation: RRID:Addgene_246173 Copy
Species: Caenorhabditis elegans
Genetic Insert: SignalPeptide-FLAG-His8-GFP-SID1(H740A_H744A)
Vector Backbone Description: Vector Backbone:pT2CAG-MCS-Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_253487 Copy
Species: Caenorhabditis elegans
Genetic Insert: 12aa linker::mScarlet (GLO)::3xHA::12aa linker
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2353; Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40463100
Comments: This plasmid contains four unique crRNA sites that can be used for re-editing the initial knock-in and inserting alternate cassettes. See this preprint for details: https://www.biorxiv.org/content/10.1101/2025.05.14.653803v1
Proper citation: RRID:Addgene_253925 Copy
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