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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pGAL-flp(H305L)-NAT Resource Report Resource Website |
RRID:Addgene_160380 | FLP1 | Saccharomyces cerevisiae | Ampicillin | NAT cassette amplified from pLND46 using primers GGT GAT GAC GGT GAA AAC CTC and CTG ATG CGG TAT TTT CTC CTT ACG . Vector flp(H305L)-URA3 (Yeast, Vol. 14: 847–852 (1998))amplified with oligos TGC GTA AGG AGA AAA TAC CGC & GTC AGA GGT TTT CAC CGT CAT outwards from the URA3 region to generate linear fragment with ends complementary to the NAT cassette. Both PCRs carried out using the Primestar MAX polymerase mix (Takara).The two products were then annealed using Gibson assembly, using 3X the amount of NAT PCR product compared to the flp PCR product. 2uL of the mix transformed into NEB 5-alpha competent cells, and transformants were miniprepped and checked for the correct marker replacement with BamH1-Nco1 restriction digest. New insert gives a smaller (1.2 kb) fragment than original vector (1.5kb fragment). Overexpression of flp(H305L) cures 2-micron plasmids. | Backbone Marker:Yeast, Vol 14: 847–852 (1998); Backbone Size:6861; Vector Backbone:pBIS-GALkFLP(URA3); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | H305L | 2026-08-15 01:24:29 | 0 | |
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pGAL-SKI1-NAT Resource Report Resource Website |
RRID:Addgene_160382 | SKI1 | Saccharomyces cerevisiae | Ampicillin | Backbone Marker:Genetics. 2009 Sep; 183(1): 365–383.; Backbone Size:5643; Vector Backbone:pLND46; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:24:29 | 0 | |||
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GFP-ATG8(416)/GFP-AUT7(416) Resource Report Resource Website |
RRID:Addgene_160389 | autophagy-related 8 | Saccharomyces cerevisiae | Ampicillin | Backbone Size:5313; Vector Backbone:pAG36; Vector Types:Yeast Expression, CEN/ARS; Bacterial Resistance:Ampicillin | 2026-08-15 01:24:29 | 0 | |||
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pUSE-URA-Sec7-mGold Resource Report Resource Website |
RRID:Addgene_218967 | SEC7-mGold | Saccharomyces cerevisiae | Ampicillin | Vector Backbone:pUSE-URA3; Vector Types:Gene replacement vector for yeast; Bacterial Resistance:Ampicillin | 2026-08-15 01:30:41 | 0 | |||
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pAM289 Resource Report Resource Website |
RRID:Addgene_226126 | Npl4 D460K/Y461A | Saccharomyces cerevisiae | Ampicillin | PMID:36736315 | Vector Backbone:pET DUET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | D460K/Y461A | 2026-08-15 01:30:41 | 0 | |
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pAM288 Resource Report Resource Website |
RRID:Addgene_226125 | Npl4 W252A/R253E | Saccharomyces cerevisiae | Ampicillin | PMID:36736315 | Vector Backbone:pET DUET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | W252A/R253E | 2026-08-15 01:30:41 | 0 | |
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pAM282 Resource Report Resource Website |
RRID:Addgene_226121 | His6-3C-Cdc48 | Saccharomyces cerevisiae | Ampicillin | PMID:36736315 | Vector Backbone:pET DUET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:30:41 | 0 | ||
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pAM290 Resource Report Resource Website |
RRID:Addgene_226127 | His6-GST-3C-Npl4 | Saccharomyces cerevisiae | Ampicillin | PMID:36736315 | Vector Backbone:pET DUET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:30:41 | 0 | ||
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pAM295 Resource Report Resource Website |
RRID:Addgene_226131 | Ufd1R64C-3C-His6 | Saccharomyces cerevisiae | Kanamycin | PMID:36736315 | Vector Backbone:pET DUET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | R64C | 2026-08-15 01:30:42 | 0 | |
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pJJB1559 Resource Report Resource Website |
RRID:Addgene_218590 | GES | Saccharomyces cerevisiae | Kanamycin | PMID:39402374 | Also expresses Idi1 and a mutant Erg20 (F96W, N127W) from Saccharomyces cerevisiae. Idi1, Erg20(F96W, N127W), and GES are targeted to the peroxisome with ePTS1. This plasmid also expresses a cytosolic, WT Erg20 synthetic homodimer in which two WT versions of the Erg20 gene are combined onto one transcript with a 6x serine-glycine linker. Integrated into the URA3 locus. | Backbone Marker:Internal; Vector Backbone:TMP138; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin | Erg20(F96W, N127W), Erg20-Erg20 synthetic homodimer fusion | 2026-08-15 01:30:49 | 0 |
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pKS1461 Resource Report Resource Website |
RRID:Addgene_218587 | Erg12 | Saccharomyces cerevisiae | Kanamycin | PMID:39402374 | Also expresses Erg8p and Mvd1p from Saccharomyces cerevisiae. Integrated into the YDR514C locus | Backbone Marker:Internal; Vector Backbone:PSG749; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:30:49 | 0 | |
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pJJB1439 Resource Report Resource Website |
RRID:Addgene_218589 | GES | Saccharomyces cerevisiae | Kanamycin | PMID:39402374 | Also expresses Idi1 and a mutant Erg20 (F96W, N127W) from Saccharomyces cerevisiae. Integrated into the URA3 locus. | Backbone Marker:Internal; Vector Backbone:TMP138; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin | Erg20(F96W, N127W) | 2026-08-15 01:30:49 | 0 |
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pJJB1402 Resource Report Resource Website |
RRID:Addgene_218616 | Pex15 | Saccharomyces cerevisiae | Kanamycin | PMID:39402374 | Express Pex15, Pex22, Pex4, Pex1, and Pex6 into the URA3 locus | Backbone Marker:Internal; Vector Backbone:TMP138; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:30:49 | 0 | |
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pJJB1401 Resource Report Resource Website |
RRID:Addgene_218615 | Pex13 | Saccharomyces cerevisiae | Kanamycin | PMID:39402374 | Express Pex13, Pex14, Pex17, Pex2, Pex10, and Pex12 into the LEU2 locus | Backbone Marker:Internal; Vector Backbone:TMP137; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:30:49 | 0 | |
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pJJB1376 Resource Report Resource Website |
RRID:Addgene_218618 | Pex5 | Saccharomyces cerevisiae | Kanamycin | PMID:39402374 | Express Pex5, Pex3, Pex19, and Pex8 into the YBR197C locus | Backbone Marker:Internal; Vector Backbone:PSG747; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:30:49 | 0 | |
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pJJB1503 Resource Report Resource Website |
RRID:Addgene_218610 | Pex30 | Saccharomyces cerevisiae | Kanamycin | PMID:39402374 | Integrated into LEU2 locus | Backbone Marker:Internal; Vector Backbone:TMP137; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:30:49 | 0 | |
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pJJB1340 Resource Report Resource Website |
RRID:Addgene_218593 | Pex22 | Saccharomyces cerevisiae | Kanamycin | PMID:39402374 | Expresses Pex22(1-36)-mRuby2 which directs mRuby2 to the peroxisome membrane. Also overexpresses Pex11p. Integrated into the LEU2 locus | Backbone Marker:Internal; Vector Backbone:TMP137; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin | Pex22(1-36) | 2026-08-15 01:30:49 | 0 |
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pSKT001 Resource Report Resource Website |
RRID:Addgene_218594 | YFP | Saccharomyces cerevisiae | Kanamycin | PMID:39402374 | Expresses the components for the degron assay: UbiY(N-terminal degradation tag)-YFP-ePTS1, directing the YFP to the peroxisome. Integrated into the IAI11 locus. | Backbone Marker:Internal; Vector Backbone:PSG746; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:30:49 | 0 | |
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pJJB170 Resource Report Resource Website |
RRID:Addgene_218591 | Adr1 | Saccharomyces cerevisiae | Kanamycin | PMID:39402374 | Expresses three engineered transcription factors (TFs): Adr1c, Pip2c, and Oaf1c for inducing peroxisome proliferation consitutively. Integrated into the LEU2 locus | Backbone Marker:Internal; Vector Backbone:TMP137; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin | Adr1(S230A), Pip2(1-168 + 2,497-2,988), Oaf1(1-300 + 2,419-3,141) | 2026-08-15 01:30:49 | 0 |
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pJJB711 Resource Report Resource Website |
RRID:Addgene_218597 | Pex11 | Saccharomyces cerevisiae | Kanamycin | PMID:39402374 | Integrated into LEU2 locus | Backbone Marker:Internal; Vector Backbone:TMP137; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:30:49 | 0 |
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