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Species: Synthetic
Genetic Insert: hTetR::P2A::EGFP::T2A::PuroR
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:7547; Vector Backbone:pDN-D2irTN2aG5kwh; Vector Types:Mammalian Expression, Synthetic Biology, Flp-In expression vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31235692
Comments: The plasmid encodes a synthetic gene circuit controlling the PuroR gene using negative feedback through a humanized tetracycline repressor (hTetR) self-repressing a CMV-derived promoter with 2 tetO2 sites (CMV-D2ir). The circuit is also called mNF-PuroR.
Proper citation: RRID:Addgene_128255 Copy
Species: Synthetic
Genetic Insert: rtTA-Advanced::P2A::EGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5070; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression, Synthetic Biology, Flp-In expression vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31235692
Comments: The plasmid encodes a mammalian synthetic gene circuit that contains the regulator reverse tetracycline Trans Activator (rtTA) and the reporter EGFP, which is controlled by a tight TRE promoter that rtTA binds to activate its own expression in a positive feedback loop. The backbone consists of the Flp-In expression vector (pcDNA5/FRT). The synthetic gene circuit is also called mPF-GFP.
Proper citation: RRID:Addgene_128254 Copy
Species: Synthetic
Genetic Insert: Beta Globin Intron::hTetR::P2A::EGFP
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:7505; Vector Backbone:pDN-D2irTNG5kwh; Vector Types:Mammalian Expression, Synthetic Biology, Flp-In expression vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31235692
Comments: The plasmid encodes a mammalian synthetic gene circuit with the humanized Tetracycline Repressor (hTetR) regulator separated from EGFP by a P2A motif, which is controlled by a CMV-derived promoter containing 2 tetO2 sites. With hTetR binding to the promoter, negative feedback lowers gene expression noise while linearizing the mean dose response. The circuit is also called mNF-GFP and is within a Flp-In expression vector.
Proper citation: RRID:Addgene_128253 Copy
Species: Homo sapiens
Genetic Insert: TEX264 LIR4A
Vector Backbone Description: Backbone Marker:Dr.Shoji Yamaoka of Tokyo Medical and Dental University; Backbone Size:6404; Vector Backbone:pMRX-INU-FLAG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31006538
Proper citation: RRID:Addgene_128259 Copy
Species: Homo sapiens
Genetic Insert: TEX264
Vector Backbone Description: Backbone Marker:Dr.Shoji Yamaoka of Tokyo Medical and Dental University; Backbone Size:6404; Vector Backbone:pMRX-INU-FLAG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31006538
Proper citation: RRID:Addgene_128258 Copy
Genetic Insert: ssRFPGFPKDEL
Vector Backbone Description: Backbone Size:7710; Vector Backbone:pCW57-CMV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31006538
Proper citation: RRID:Addgene_128257 Copy
Species: Homo sapiens
Genetic Insert: ZNF326_iso1 gRNA
Vector Backbone Description: Vector Backbone:PX458; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26355004
Proper citation: RRID:Addgene_128244 Copy
Species: Homo sapiens
Genetic Insert: ZNF326_iso1 gRNA
Vector Backbone Description: Vector Backbone:PX458; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26355004
Proper citation: RRID:Addgene_128243 Copy
Species: Homo sapiens
Genetic Insert: THRA_iso1 gRNA
Vector Backbone Description: Vector Backbone:PX458; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26355004
Proper citation: RRID:Addgene_128245 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GRE-lacZ
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS425(LEU2, 2u); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7761857
Proper citation: RRID:Addgene_1282 Copy
Species: Homo sapiens
Genetic Insert: ZHX3_iso1 gRNA
Vector Backbone Description: Vector Backbone:PX458; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26355004
Proper citation: RRID:Addgene_128249 Copy
Species: Vaccinia Virus
Genetic Insert: VV.Orf134
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA 3.1D V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15623576
Comments: Please see the Vaccinia kit page ( https://www.addgene.org/vvorf/ ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid.
Proper citation: RRID:Addgene_12822 Copy
Species: Vaccinia Virus
Genetic Insert: VV.Orf132
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA 3.1D V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15623576
Comments: Please see the Vaccinia kit page ( https://www.addgene.org/vvorf/ ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid.
Proper citation: RRID:Addgene_12820 Copy
Species: MS2 Phage
Genetic Insert: Maturation Protein
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:2200; Vector Backbone:pMX; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31618483
Proper citation: RRID:Addgene_128233 Copy
Species: Synthetic
Genetic Insert: classic sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)
Vector Backbone Description: Backbone Marker:Twist Biosciences; Vector Backbone:pTwist-Kan; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128198 Copy
Species: Homo sapiens
Genetic Insert: lamin A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5500; Vector Backbone:pCMS-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31006814
Comments: lamin A splicing reporter with short-lived GFP downstream LMNA exon 12 (CMV-driven) + SV40-driven dsRed2 to identified transfected cells. C>T mutation in exon 11. The mutation causes aberrant splicing event, creating a mutant protein, delta50 lamin A.
Proper citation: RRID:Addgene_128231 Copy
Species: Synthetic
Genetic Insert: ZmUBIp::nScCas9 (wheat codon optimized):NOSt
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128197 Copy
Species: Homo sapiens
Genetic Insert: lamin A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5500; Vector Backbone:pCMS-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31006814
Comments: WT lamin A splicing reporter with short-lived GFP downstream LMNA exon 12 (CMV-driven) + SV40-driven dsRed2 to identified transfected cells.
Proper citation: RRID:Addgene_128230 Copy
Species: MS2 Phage
Genetic Insert: Maturation Protein
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:2200; Vector Backbone:pMX; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31618483
Proper citation: RRID:Addgene_128234 Copy
Species: Homo sapiens
Genetic Insert: ZHX3_iso1 Homology arms
Vector Backbone Description: Vector Backbone:pFETCh_Donor; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26355004
Proper citation: RRID:Addgene_128239 Copy
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