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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pEGFP-Nck1 (M3) Resource Report Resource Website |
RRID:Addgene_45896 | Nck-1 M3 mutant | Homo sapiens | Kanamycin | PMID:11959995 | Wild type pEGFP-Nck1 (Addgene plasmid 45903) is described in JBC 26:26633-44 2006 (PMID 16835242). Mutations are described in the article linked to this plasmid (PMID 11959995) and then subcloned into the pEGFP-C1 vector using PCR amplification with primers containing XhoI and EcoRI sites. | Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | M3 (W229R) in SH3 domain | 2026-08-15 01:15:27 | 0 |
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pBEST-PLlacO-1-UTR1-TetR-YbaQ-T500 Resource Report Resource Website |
RRID:Addgene_45778 | Tetracycline repressor | Ampicillin | Backbone Marker:Promega; Vector Backbone:pBEST-Luc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:26 | 0 | ||||
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hα1Ga-pDsRed Resource Report Resource Website 1+ mentions |
RRID:Addgene_45811 | hα1Ga | Homo sapiens | Kanamycin | The Cav3.1 insert retains its STOP codon, so there is no expression of the Ds-Red. The 5'UTR is gone and contains a new SalI site. The 3' end of insert ends in the endogenous Kpn1 site. Identification of Cav3.1 is described in Nature. 1998 Feb 26;391(6670):896-900. | Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pDsRed-Express-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:15:26 | 5 | ||
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pSV HA eIF2beta (Pro110-113) Resource Report Resource Website |
RRID:Addgene_45899 | eIF2beta | Mus musculus | Bleocin (Zeocin) | PMID:11959995 | Two fragments were PCR amplified from GST-eIF2beta (Addgene plasmid 45900) and ligated together with vector to incorporate deletion. Xba1 site is used to ligate the 2 parts of eIF2beta. | Backbone Marker:Invitrogen; Backbone Size:3500; Vector Backbone:pZeoSV2; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin) | P110A, P113A | 2026-08-15 01:15:27 | 0 |
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hE-cadherin/Δp120 Resource Report Resource Website 1+ mentions |
RRID:Addgene_45770 | hE-cadherin/Δp120 | Homo sapiens | Ampicillin | PMID:11381089 | The three-alanine substitution present in this plasmid prevent the hE-cadherin gene product from interacting with p120 catenin. Alternate plasmid name: hE-cadherin-762AAA-pcDNA3 | Vector Backbone:pLKpac1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Amino acids 762-764 converted from EED to AAA to abolish p120 interaction | 2026-08-15 01:15:26 | 2 |
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YCplac111-yVps23-mCherry Resource Report Resource Website |
RRID:Addgene_45925 | yeast Vps23 | Saccharomyces cerevisiae | Ampicillin | PMID:21505419 | Backbone Size:6103; Vector Backbone:YCplac111; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:27 | 0 | ||
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pMRXIP GFP-VAMP7 Resource Report Resource Website |
RRID:Addgene_45920 | vesicle-associated membrane protein 7 | Mus musculus | Ampicillin | PMID:23217709 | Backbone Marker:Dr.Shoji Yamaoka of Tokyo Medical and Dental University; Backbone Size:6600; Vector Backbone:pMRXIP GFP-Ci2; Vector Types:Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:27 | 0 | ||
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pMRXIP GFP-VAMP8 Resource Report Resource Website 1+ mentions |
RRID:Addgene_45919 | vesicle-associated membrane protein 8 | Homo sapiens | Ampicillin | PMID:23217709 | Backbone Marker:Dr.Shoji Yamaoka of Tokyo Medical and Dental University; Backbone Size:6600; Vector Backbone:pMRXIP GFP-Ci2; Vector Types:Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:27 | 1 | ||
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pNMHCII-C1-GFP Resource Report Resource Website |
RRID:Addgene_46041 | nonmuscle myosin heavy chain II-C, isoform C1 | Mus musculus | Kanamycin | PMID:16790446 | Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:15:28 | 0 | ||
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pNMHCII-C1C2-GFP Resource Report Resource Website |
RRID:Addgene_46044 | nonmuscle myosin heavy chain II-C, isoform C1C2 | Mus musculus | Kanamycin | PMID:19240025 | Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:15:28 | 0 | ||
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pHIS2-KL EAT Resource Report Resource Website |
RRID:Addgene_46042 | Atg1 EAT domain | K. lactis | Ampicillin | PMID:23219485 | Backbone Size:5550; Vector Backbone:pHIS-parallel.2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:28 | 0 | ||
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pOSIP-TH (TetR, HK022) Resource Report Resource Website |
RRID:Addgene_45988 | Tetracycline | PMID:24050148 | Reference: St-Pierre F, Cui L et al., "One-step cloning and chromosomal integration of DNA", ACS Synthetic Biology, http://pubs.acs.org/doi/abs/10.1021/sb400021j | Backbone Size:6925; Vector Backbone:pOSIP; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline | 2026-08-15 01:15:28 | 0 | |||
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pMM287 Resource Report Resource Website |
RRID:Addgene_46035 | WRN K577M | Homo sapiens | Ampicillin | PMID:15084309 | Bidirectional vector expresses WRN and GFP at similar expression levels. In the associated article, cells were transiently transfected with this plasmid together with a tTA transactivator plasmid (Gossen, 1992). This is a mammalian expression vector for helicase-dead human WRN protein. It was constructed from pBI, a Clontech plasmid with a bi-directional promoter+ TRE backbone, modified by insertion of a polylinker containing BspD1 and Xhol sites followed by insertion of a myc-WRN K577M ORF- created by making K577M (A>T) mutation in pMM290 sequence. The S2G mutation was a result of the cloning strategy to add the myc tag. The WRN protein containing S2G was biochemically verified and is wild-type in terms of activities, as described in the associated publication. | Backbone Marker:Clontech; Vector Backbone:pBI; Vector Types:Mammalian Expression, TetOn, bidirectional promoter; Bacterial Resistance:Ampicillin | K577M helicase-dead mutant; S2G introduced during cloning | 2026-08-15 01:15:28 | 0 |
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pOSIP-KC (KanR, PhiC31) Resource Report Resource Website |
RRID:Addgene_45982 | Kanamycin | PMID:24050148 | Reference: St-Pierre F, Cui L et al., "One-step cloning and chromosomal integration of DNA", ACS Synthetic Biology, http://pubs.acs.org/doi/abs/10.1021/sb400021j | Backbone Size:7145; Vector Backbone:pOSIP; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:15:28 | 0 | |||
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tetO-GATA4 Resource Report Resource Website 1+ mentions |
RRID:Addgene_46030 | GATA4 | Homo sapiens | Ampicillin | PMID:23591016 | Please note that Addgene's sequencing identified R43Q, G50S and N197S mutations in the GATA4 insert in this plasmid when compared to GenBank reference sequence NP_002043.2. These difference were present in the original GATA4 sample and the plasmid functions as described in the associated publication. | Vector Backbone:tetO-Gateway; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | R43Q, G50S and N197S | 2026-08-15 01:15:28 | 6 |
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tetO-MEF2C Resource Report Resource Website 1+ mentions |
RRID:Addgene_46031 | MEF2C | Homo sapiens | Ampicillin | PMID:23591016 | Vector Backbone:tetO-Gateway; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:28 | 4 | ||
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pE-FLP Resource Report Resource Website 10+ mentions |
RRID:Addgene_45978 | flp | Ampicillin | PMID:24050148 | Can be used to remove the integration module (including the antibiotic resistance cassette) from pOSIP integrants. Reference: St-Pierre F, Cui L et al., "One-step cloning and chromosomal integration of DNA", ACS Synthetic Biology, http://pubs.acs.org/doi/abs/10.1021/sb400021j | Vector Backbone:pKD46; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | flp driven by the constitutive promoter pE from phage P2 | 2026-08-15 01:15:28 | 15 | |
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pGEX-parkin W403A-C431S Resource Report Resource Website |
RRID:Addgene_45977 | Parkin W403A-C431S | Rattus norvegicus | Ampicillin | PMID:23661642 | Backbone Marker:Amersham; Backbone Size:4966; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Codon usage optimized for E.coli expression. Double mutation W403A-C431S | 2026-08-15 01:15:28 | 0 | |
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TroponinT-Gateway Resource Report Resource Website |
RRID:Addgene_46026 | Chloramphenicol and Ampicillin | PMID:23591016 | Please note that Addgene's sequencing results identified nucleotide mismatches at bp# 3473, 3937, 4087 & 4822 when compared to the full plasmid sequence. According to the depositing laboratory, these differences are not a concern for the function of the plasmid. | Vector Backbone:FUdeltaGW-rtTA; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:15:28 | 0 | |||
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pGEX-parkin 219-465 Resource Report Resource Website |
RRID:Addgene_45972 | Parkin 219-465 | Rattus norvegicus | Ampicillin | PMID:23661642 | Backbone Marker:Amersham; Backbone Size:4966; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Codon usage optimized for E.coli expression. Deleted a.a. 1-219 | 2026-08-15 01:15:28 | 0 |
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