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Species: Synthetic
Genetic Insert: Superfolder GFP
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:5653; Vector Backbone:pRBC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31243963
Proper citation: RRID:Addgene_174077 Copy
Species: Synthetic
Genetic Insert: human codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
Vector Backbone Description: Vector Backbone:JDS246 (Plasmid #43861); Vector Types:Mammalian Expression, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_174137 Copy
Species: Synthetic
Genetic Insert: eABE
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34732717
Proper citation: RRID:Addgene_174120 Copy
Species: Synthetic
Genetic Insert: HypaR-ABE8e
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34732717
Proper citation: RRID:Addgene_174124 Copy
Species: Synthetic
Genetic Insert: Candida optimized monomeric GFP
Vector Backbone Description: Backbone Size:2242; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34730409
Proper citation: RRID:Addgene_174434 Copy
Species: Synthetic
Genetic Insert: RFP with PAM rich sequence upstream promoter
Vector Backbone Description: Backbone Size:3396; Vector Backbone:SC101; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33823546
Proper citation: RRID:Addgene_174369 Copy
Species: Synthetic
Genetic Insert: Alpha N-terminal domain from the E. coli RNA polymerase fused to SYNZIP17
Vector Backbone Description: Backbone Size:1925; Vector Backbone:CloDF13; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33823546
Proper citation: RRID:Addgene_174367 Copy
Species: Synthetic
Genetic Insert: 3C-6xHIS
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_174590 Copy
Species: Synthetic
Genetic Insert: Twin Streptavidin uncleavable
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_174591 Copy
Species: Synthetic
Genetic Insert: 6xHIS uncleavable
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_174589 Copy
Species: Synthetic
Genetic Insert: Twin Streptavidin uncleavable
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_174586 Copy
Species: Synthetic
Genetic Insert: 6xHIS-GB1
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_174587 Copy
Species: Synthetic
Genetic Insert: attP and 3xP3-GFP-SV40 BsaI cassette
Vector Backbone Description: Backbone Size:2961; Vector Backbone:pKSB-; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: attP site within the fluorescence marker cassette is to allow subsequent insertions of attB plasmids in the knock-in locus
Proper citation: RRID:Addgene_174534 Copy
Species: Synthetic
Genetic Insert: PUb-mTurquoise BsaI cassette
Vector Backbone Description: Backbone Size:2961; Vector Backbone:pKSB-; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: The added N-terminal NLS in mTurquoise does not work very well, fluorescence is mostly cytoplasmic
Proper citation: RRID:Addgene_174535 Copy
Species: Synthetic
Genetic Insert: 3xP3-GFP BsaI cassette
Vector Backbone Description: Backbone Size:2961; Vector Backbone:pKSB-; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: The Tub56D terminator was chosen as an alternative to SV40
Proper citation: RRID:Addgene_174531 Copy
Species: Synthetic
Genetic Insert: 3xP3-DsRed BsaI cassette
Vector Backbone Description: Backbone Size:2961; Vector Backbone:pKSB-; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_174532 Copy
Species: Synthetic
Genetic Insert: 3xP3-mTurquoise BsaI cassette
Vector Backbone Description: Backbone Size:2961; Vector Backbone:pKSB-; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_174533 Copy
Species: Synthetic
Genetic Insert: LUC2
Vector Backbone Description: Backbone Marker:VectorBuilder; Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_174665 Copy
Species: Synthetic
Genetic Insert: mtrCAB
Vector Backbone Description: Backbone Size:3903; Vector Backbone:pET-28a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: Please note MtrC starts at amino acid 75. Depositor confirms this is not a concern.
Proper citation: RRID:Addgene_174618 Copy
Species: Synthetic
Genetic Insert: xXFKBP homo-mKeima-2x FKBP homo-2x FKBP
Vector Backbone Description: Vector Backbone:B-actin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34447998
Comments: Internal plasmid ID: GK21 bioRxiv 2021.04.21
Proper citation: RRID:Addgene_174650 Copy
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