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Species: Other
Genetic Insert: dnaQ926 RBS+ dam seqA emrR ugi CDA1
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69700 Copy
Species: Other
Genetic Insert: dnaQ926 dam seqA yffI
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69673 Copy
Species: Other
Genetic Insert: dnaQ926 dam seqA rsmE
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69671 Copy
Species: Other
Genetic Insert: dnaQ926 dam seqA nrdAB
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69678 Copy
Species: Other
Genetic Insert: dnaQ926 dam seqA ugi CDA1
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69677 Copy
Species: Other
Genetic Insert: dnaQ926 dam seqA nrdAB(P334L)
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69682 Copy
Species: Other
Genetic Insert: dnaQ926 dam seqA nrdA(A65V)B
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69680 Copy
Species: Other
Genetic Insert: dnaQ926 dam seqA emrR ugi CDA1 (opt)
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69689 Copy
Species: Other
Genetic Insert: dnaQ926 dam seqA ugi APOBEC (opt)
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69685 Copy
Vector Backbone Description: Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27084942
Proper citation: RRID:Addgene_69779 Copy
Vector Backbone Description: Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27084942
Comments: Please note that parental strain (MC4100) is streptomycin-resistant.
Proper citation: RRID:Addgene_69786 Copy
Species: Francisella tularensis subsp. novicida
Genetic Insert: FnCpf1 locus without Cas genes
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:4245; Vector Backbone:pACYC184; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26422227
Proper citation: RRID:Addgene_69974 Copy
Species: Francisella tularensis subsp. novicida
Genetic Insert: FnCpf1 locus
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:4245; Vector Backbone:pACYC184; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26422227
Proper citation: RRID:Addgene_69973 Copy
Genetic Insert: Lasso 1
Vector Backbone Description: Backbone Marker:Registry of Standard Biological Parts; Backbone Size:2060; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25967060
Comments: Please see genebank file for annotations.
Proper citation: RRID:Addgene_69935 Copy
Species: Homo sapiens
Genetic Insert: Pumilio homology domain amino acids 25-60
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2280; Vector Backbone:pChlr-pNEB193; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24581042
Proper citation: RRID:Addgene_52139 Copy
Species: Homo sapiens
Genetic Insert: Pumilio homology domain amino acids 61-96
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2280; Vector Backbone:pChlr-pNEB193; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24581042
Proper citation: RRID:Addgene_52145 Copy
Species: Homo sapiens
Genetic Insert: Pumilio homology domain amino acids 25-60
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2280; Vector Backbone:pChlr-pNEB193; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24581042
Proper citation: RRID:Addgene_52142 Copy
Species: Homo sapiens
Genetic Insert: Pumilio homology domain amino acids 61-96
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2280; Vector Backbone:pChlr-pNEB193; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24581042
Proper citation: RRID:Addgene_52143 Copy
Species: Homo sapiens
Genetic Insert: Pumilio homology domain amino acids 97-132
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2280; Vector Backbone:pChlr-pNEB193; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24581042
Proper citation: RRID:Addgene_52149 Copy
Species: Homo sapiens
Genetic Insert: Pumilio homology domain amino acids 97-132
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2280; Vector Backbone:pChlr-pNEB193; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24581042
Proper citation: RRID:Addgene_52150 Copy
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