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http://www.addgene.org/117702

Species: Mus musculus
Genetic Insert: Sod1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin

Proper citation: RRID:Addgene_117702 Copy   

•   Source: Addgene
  

http://www.addgene.org/117709

Species: Mus musculus
Genetic Insert: Sod1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin

Proper citation: RRID:Addgene_117709 Copy   

•   Source: Addgene
  

http://www.addgene.org/117708

Species: Mus musculus
Genetic Insert: Sod1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin

Proper citation: RRID:Addgene_117708 Copy   

•   Source: Addgene
  

http://www.addgene.org/117790

Species: Danio rerio
Genetic Insert: Cre
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a gamma-crystalin promoter driving NLS-mRFP. Online tools and cloning help available at . May work in additional species beyond those listed. Please note that some discrepancies were found between Addgene's quality control result and the depositor's provided sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

Proper citation: RRID:Addgene_117790 Copy   

•   Source: Addgene
  

http://www.addgene.org/11779

Genetic Insert: CAG promoters, GFP, rtTR-KRAB-2SM2, Tet-off
Vector Backbone Description: Backbone Size:12859; Vector Backbone:None; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16432520
Comments: Transgenes can be expressed from any RNA Pol II promoter as part of bicistronic unit comprising the KRAB-based repressor; tetO sequences are inserted into the vector LTR. Tet-on and Tet-off versions rely on repressors that bind in the absence or the presence of doxycycline, respectively. Addition of Pol III promoter-small hairpin RNA cassette allows for drug-controllable RNA interference (Tet-on shRNA). Cloning shRNA into pLVET, pLVCT, and pLVPT vectors: first clone shRNA into pLVTHM downstream of the tetO-H1 region. Then cut pLVTHM containing your shRNA with MscI-FspI and clone the insert containing the 3'LTR to target plasmid opened with MscI-FspI. FspI cuts into AmpR. This means for inverted clones AmpR will not be restored; after selection you will be left with clones with the shRNA cassette and functional AmpR. pLVTHM and packaging plasmid for this system are also available at Addgene http://www.addgene.org/rnaitools Please visit Trono lab website http://tronolab.epfl.ch to see frequently asked questions on cloning strategies and packaging.

Proper citation: RRID:Addgene_11779 Copy   

•   Source: Addgene
  

http://www.addgene.org/117773

Species: Danio rerio
Genetic Insert: 3x3 Stop sequence
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a gamma-crystalin promoter driving NLS-eGFP. Online tools and cloning help available at . May work in additional species beyond those listed. Please note that some discrepancies were found between Addgene's quality control result and the depositor's provided sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

Proper citation: RRID:Addgene_117773 Copy   

•   Source: Addgene
  

http://www.addgene.org/117775

Species: Danio rerio
Genetic Insert: 3x3 Stop sequence
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-mRFP, Oline tools and cloning help avalible at . May work in additional species beyond those listed. Please note that some discrepancies were found between Addgene's quality control result and the depositor's provided sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

Proper citation: RRID:Addgene_117775 Copy   

•   Source: Addgene
  

http://www.addgene.org/117772

Species: Danio rerio
Genetic Insert: 3x3 Stop sequence
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a gamma-crystalin promoter driving NLS-mRFP. Online tools and cloning help available at . May work in additional species beyond those listed. Please note that some discrepancies were found between Addgene's quality control result and the depositor's provided sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

Proper citation: RRID:Addgene_117772 Copy   

•   Source: Addgene
  

http://www.addgene.org/117778

Species: Danio rerio
Genetic Insert: gal4/VP16
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a gamma-crystalin promoter driving NLS-mRFP. Online tools and cloning help available at . gal4-VP16 should be used in conjunction with a UAS expression line. May work in additional species beyond those listed. Please note that some discrepancies were found between Addgene's quality control result and the depositor's provided sequence. The depositor noted that these discrepancies do NOT affect plasmid function. This plasmid has been found exist in our stock as a multimer. Plasmid multimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.

Proper citation: RRID:Addgene_117778 Copy   

•   Source: Addgene
  

http://www.addgene.org/117884

Species: Arabidopsis thaliana
Genetic Insert: AtIRT1
Vector Backbone Description: Backbone Size:3329; Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Comments: insert amino acid sequence: MASNSALLMKTIFLVLIFVSFAISPATSTAPEECGSESANPCVNKAKALPLKVIAIFVILIASMIGVGAPLFSRNVSFLQPDGNIFTIIKCFASGIILGTGFMHVLPDSFEMLSSICLEENPWHKFPFSGFLAMLSGLITLAIDSMATSLYTSKNAVGIMPHGHGHGHGPANDVTLPIKEDDSSNAQLLRYRVIAMVLELGIIVHSVVIGLSLGATSDTCTIKGLIAALCFHQMFEGMGLGGCILQAEYTNMKKFVMAFFFAVTTPFGIALGIALSTVYQDNSPKALITVGLLNACSAGLLIYMALVDLLAAEFMGPKLQGSIKMQFKCLIAALLGCGGMSIIAKWA

Proper citation: RRID:Addgene_117884 Copy   

•   Source: Addgene
  

http://www.addgene.org/117885

Species: Arabidopsis thaliana
Genetic Insert: AtZIF1
Vector Backbone Description: Backbone Size:3329; Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Comments: insert amino acid sequence: MAEEYKEALLEKQNYHDGCPGCKVEQMKQLRRGYPYLELSFVWIIVLSTSLPISSLYPFLYYMIEDFGVAKTEKDIGFYAGFVGCSFMLGRALTSVFWGIVADRYGRKPIILLGTISIAIFNALFGLSSNFWMAIGTRFLLGSFNCLLGTMKAYASEIFRDEYQATAMSAVSTAWGIGLIIGPALGGFLAQPADKYPNVFSQESLFGRFRYALPCFTISAFALLVTVLCCFIPETLHNHKLDSLSHDDSYDILEAASHESSPSTGKAGKNERKASQSLLKNWPLMSSIIVYCVLCLHDTAYSEIFALWANSPRKYGGLSYSTNEVGTVLAISGLGLFSFQVFVYPLAEKLLGPVLVTRYAGALMIPIQMSYPFIAGLSGLSLSLMLNCASILINVLSVSAITGLLILQNRAVDQSQRGAANGIAMTAMSLFKTVGPAGAGILFSWSERRLNAAFLPGSHMVFFVLNVIVVVGVALTFKPFLTTSRR

Proper citation: RRID:Addgene_117885 Copy   

•   Source: Addgene
  

http://www.addgene.org/117880

Species: Arabidopsis thaliana
Genetic Insert: CHL1T101D-eGFP
Vector Backbone Description: Backbone Size:3329; Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)

Proper citation: RRID:Addgene_117880 Copy   

•   Source: Addgene
  

http://www.addgene.org/117803

Species: Homo sapiens
Genetic Insert: CENP-A
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11129795
Comments: The insert fragment with Sac I and Xho I sites was cloned into Sac I and Sal I sites of pEGFP-C1. Thus, there is no Xho I and Sal I site in pEGFP-AF8. The stop codon was also changed to TCG (..ctcggcTCGac-3’) Note that the cloning strategy adds additional amino acids onto the C-terminus of the insert (STVPRARDPPDLDN), but this does not interfere with the function.

Proper citation: RRID:Addgene_117803 Copy   

•   Source: Addgene
  

http://www.addgene.org/117887

Species: Arabidopsis thaliana
Genetic Insert: AtALMT1-upGFP 5'
Vector Backbone Description: Backbone Size:3329; Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)

Proper citation: RRID:Addgene_117887 Copy   

•   Source: Addgene
  

http://www.addgene.org/117889

Species: Arabidopsis thaliana
Genetic Insert: AtALMT1-upGFP 3'
Vector Backbone Description: Backbone Size:3329; Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)

Proper citation: RRID:Addgene_117889 Copy   

•   Source: Addgene
  

http://www.addgene.org/117808

Species: Homo sapiens
Genetic Insert: TagRFP
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2958; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Online tools and cloning help available at . May work in additional species beyond those listed. Please visit https://www.biorxiv.org/content/10.1101/431627v2 for bioRxiv preprint.

Proper citation: RRID:Addgene_117808 Copy   

•   Source: Addgene
  

http://www.addgene.org/117807

Species: Homo sapiens
Genetic Insert: TagRFP
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2958; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Online tools and cloning help available at . May work in additional species beyond those listed. Please visit https://www.biorxiv.org/content/10.1101/431627v2 for bioRxiv preprint.

Proper citation: RRID:Addgene_117807 Copy   

•   Source: Addgene
  

http://www.addgene.org/117872

Species: Arabidopsis thaliana
Genetic Insert: AtALMT-uPIC
Vector Backbone Description: Backbone Size:3329; Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)

Proper citation: RRID:Addgene_117872 Copy   

•   Source: Addgene
  

http://www.addgene.org/117874

Species: Zea mays
Genetic Insert: NiaP
Vector Backbone Description: Backbone Size:3329; Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Comments: insert amino acid sequence: MAPEMMEEGQSASYTVDDALLSSGFGRFQILILSYAGIGLIAEAMEMMLLSFVGPSVQLEWKLTSHQESMITSVVFVGMLIGAYSWGVVSDNYGRRRGFLFTAIMTSGAGFFSAFAPNYLSLISLRFLVGIGLGGGPVLGSWFLEFVPAPTRGTWMVVFSAFWTVGTILEASLAWTIMPKFGWRWLLALSAVPSFLLLLFYAITPESPRFLCMKGRTTEAVDILEKMARLNNVQLPSGRLVSDKNIELDEVSGSSESTTLLAGAEESDNLNEDQGSDFGGIKSVGKLLAPKLIRATLLLWMAFFGNAFAYYGIVLLTSELSNGNRICAKEDVESVHSTNASLYKNVFISSFAEIPGSFLSAMIVDRFGRKRSMASMLFTSCVFLLPLVFSRTDILTRISLFGARLCISASFTIVYIYAPEIYPTAVRTTGIGIASSVGRIGGILCPLVAVALVHSCQQTTAILLFELVIFLSGLAVSFFPFETKGCRLNDTDVDMN

Proper citation: RRID:Addgene_117874 Copy   

•   Source: Addgene
  

http://www.addgene.org/117877

Species: Arabidopsis thaliana
Genetic Insert: CHL1 T101A
Vector Backbone Description: Backbone Size:3329; Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)

Proper citation: RRID:Addgene_117877 Copy   

•   Source: Addgene