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Species: Other
Genetic Insert: mCherry with early endosome-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174452 Copy
Species: Other
Genetic Insert: iRFP with early endosome-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174453 Copy
Species: Other
Genetic Insert: EGFP with early endosome-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174454 Copy
Species: Other
Genetic Insert: iRFP with early endosome-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174456 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2349; Vector Backbone:pENTR1A; Vector Types:Mammalian Expression, Bacterial Expression, Yeast Expression, Insect Expression, Plant Expression, modified Gateway Entry vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18211686
Comments: Part of a modified Gateway Entry vector system for epitope tagging. Clone your gene of interest into this vector using the BamHI and NotI cloning sites to obtain a N-terminal STREP tagged Entry vector. The resulting Entry vector can be recombined into Gateway Destination vectors for bacteria, insect mammalian, plant or yeast expression via the Gateway LR reaction.
The tag is present in the Entry cassette to avoid the presence of att-derived protein linker sequences between the tag and gene or interest corresponding to the attB recombination site. A stop codon is already present within the vector (see plasmid map).
GenBank accession number: EU334816
Proper citation: RRID:Addgene_17441 Copy
Species: Homo sapiens
Genetic Insert: small heat shock protein27
Vector Backbone Description: Backbone Marker:BD Biosciences Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17597071
Proper citation: RRID:Addgene_17444 Copy
Species: Other
Genetic Insert: SECFP with golgi apparatus-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174470 Copy
Species: Other
Genetic Insert: Venus with endoplasmic reticulum-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174468 Copy
Species: Other
Genetic Insert: Sirius with golgi apparatus-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174469 Copy
Species: Other
Genetic Insert: EGFP with recycling endosome-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174460 Copy
Species: Other
Genetic Insert: iRFP with recycling endosome-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174462 Copy
Species: Other
Genetic Insert: Sirius with endoplasmic reticulum-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174464 Copy
Species: Other
Genetic Insert: SECFP with endoplasmic reticulum-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174465 Copy
Species: Other
Genetic Insert: Sirius with endoplasmic reticulum-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174466 Copy
Species: Other
Genetic Insert: Venus with endoplasmic reticulum-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174467 Copy
Species: Other
Genetic Insert: protein A - hia5
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35396487
Comments: The depositing laboratory recommends Addgene Plasmid 222303: prhaBAD-pA-Hia5, an improved version of this plasmid.
Protocol for purification and expression can be found at: dx.doi.org/10.17504/protocols.io.bv82n9ye
Application for DiMeLo-seq is described in: doi.org/10.1101/2021.07.06.451383
Protocol was adapted and modified from: doi.org/10.1126/science.aaz1646
Proper citation: RRID:Addgene_174372 Copy
Species: Synthetic
Genetic Insert: RFP with PAM rich sequence upstream promoter
Vector Backbone Description: Backbone Size:3396; Vector Backbone:SC101; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33823546
Proper citation: RRID:Addgene_174369 Copy
Species: Salmonella enterica ser. Typhimurium
Genetic Insert: sarA
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31901521
Proper citation: RRID:Addgene_174400 Copy
Species: Salmonella enterica ser. Typhimurium
Genetic Insert: sarA
Vector Backbone Description: Vector Backbone:pWSK129; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31901521
Proper citation: RRID:Addgene_174401 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3313; Vector Backbone:pENTR1A; Vector Types:RNAi, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17453 Copy
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