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Species: Saccharomyces cerevisiae
Genetic Insert: Fyv6
Vector Backbone Description: Vector Backbone:pRS414; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234134 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Fyv6
Vector Backbone Description: Vector Backbone:pRS414; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234136 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Fyv6
Vector Backbone Description: Vector Backbone:pRS414; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234137 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PHO8∆60
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40458443
Proper citation: RRID:Addgene_240971 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: BleMX6
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40458443
Proper citation: RRID:Addgene_240974 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VPS34
Vector Backbone Description: Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26450213
Proper citation: RRID:Addgene_235751 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VPS38
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26450213
Proper citation: RRID:Addgene_235758 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ATG38
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27630019
Proper citation: RRID:Addgene_242493 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ATG38
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27630019
Proper citation: RRID:Addgene_242491 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ATG38
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27630019
Comments: Addgene found the insertion of an IS4 transposase like element in the backbone, downstream of T3 promoter. This discrepancy is not likely to affect plasmid function.
Proper citation: RRID:Addgene_242492 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ATG38
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27630019
Proper citation: RRID:Addgene_242489 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PHO8∆60
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40458443
Proper citation: RRID:Addgene_240970 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FLP Recombinase
Vector Backbone Description: Backbone Marker:NA; Backbone Size:5209; Vector Backbone:pET28a(+)v2; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: We use a tag-free SUMO protease (ulp1) for cleaving the TwinStrep tag from the final product. Tag-free ulp1 can be synthesized using Addgene Plasmid #190063 and a His-tagged TEV protease or HRV-3C protease (PreScission protease).
The total insert size displayed on this page (1872bp) includes all tags and linkers.
Proper citation: RRID:Addgene_246980 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: msGFP2-Vrg4
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_247743 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sys1-FRBx2
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_247750 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sys1-HaloTagx2
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_247751 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gnt1-HaloTag
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_247736 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gnt1-moxGFP2
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_247737 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HaloTag-Vrg4
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_247738 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VPS34
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26450213
Proper citation: RRID:Addgene_235750 Copy
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