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Species: Rhodosporidium toruloides
Genetic Insert: gRNA cloning cassette
Vector Backbone Description: Backbone Size:6303; Vector Backbone:pZPK; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31038202
Proper citation: RRID:Addgene_128178 Copy
Species: Synthetic
Genetic Insert: improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128211 Copy
Species: Rhodosporidium toruloides
Genetic Insert: SpCas9-NLS3
Vector Backbone Description: Backbone Marker:Zhao lab; Backbone Size:13658; Vector Backbone:pCAMBRIA; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31038202
Proper citation: RRID:Addgene_128177 Copy
Species: Synthetic
Genetic Insert: improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128210 Copy
Species: Homo sapiens
Genetic Insert: shcGAS Mmu
Vector Backbone Description: Backbone Size:13320; Vector Backbone:pTRIPZ RFP+; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28598415
Proper citation: RRID:Addgene_128175 Copy
Species: Synthetic
Genetic Insert: improved sgRNA backbone for tRNA-gRNA array, Position 2
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128215 Copy
Species: Synthetic
Genetic Insert: improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128214 Copy
Species: Synthetic
Genetic Insert: improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128213 Copy
Species: Synthetic
Genetic Insert: sgRNA#1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pX330; Vector Types:CRISPR, Toxoplasma gondii expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31243081
Proper citation: RRID:Addgene_128179 Copy
Species: Synthetic
Genetic Insert: improved sgRNA backbone for tRNA-gRNA array, Position 5
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128218 Copy
Species: Synthetic
Genetic Insert: improved sgRNA backbone for tRNA-gRNA array, Position 4
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128217 Copy
Species: Synthetic
Genetic Insert: mTagBFP2-T2A-iCreERT2
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128173 Copy
Species: Synthetic
Genetic Insert: mTagBFP2-T2A-iCreERT2
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_128171 Copy
Species: Synthetic
Genetic Insert: mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32183906
Comments: Linker between sfGFP and iCre-ERT2 is a clean GSAx9 sequence, similar in length to the mutated P2A sequence in the plasmid pCAG-sfGFP-iCre-ERT2 (#125749).
The sfGFP fluorescence from the sfGFP-iCre-ERT2 is dim, as described in Nam and Capecchi, 2020 (https://doi.org/10.1186/s13064-020-00139-5) and a Figshare page (https://doi.org/10.6084/m9.figshare.13218437.v35). In short, it is not visible to the eyes, but can be detected with flow cytometry.
Proper citation: RRID:Addgene_128167 Copy
Species: Synthetic
Genetic Insert: mTagBFP2-T2A-iCreERT2
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32183906
Comments: The depositor recommends the updated versions of this plasmid, PB CAG-sfGFP-T2A-iCreERT2 (https://www.addgene.org/177309/) or PB CAG-mTagBFP2-T2A-iCreERT2 (https://www.addgene.org/177308/).
Proper citation: RRID:Addgene_128168 Copy
Species: Synthetic
Genetic Insert: sgRNA backbone for SaCas9, combine with TaU3 promoter module (pFH33)
Vector Backbone Description: Backbone Marker:Twist Biosciences; Vector Backbone:pTwist-Kan; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128201 Copy
Species: Synthetic
Genetic Insert: crRNA backbone for LbCas12a, combine with AtU6-26 promoter module (pFH35)
Vector Backbone Description: Backbone Marker:Twist Biosciences; Vector Backbone:pTwist-Kan; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128208 Copy
Species: Synthetic
Genetic Insert: sgRNA backbone for StCas9, combine with AtU6-26 promoter module (pFH35)
Vector Backbone Description: Backbone Marker:Twist Biosciences; Vector Backbone:pTwist-Kan; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128206 Copy
Species: Vaccinia Virus
Genetic Insert: VV.Orf129
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA 3.1D V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15623576
Comments: Please see the Vaccinia kit page ( https://www.addgene.org/vvorf/ ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid.
Proper citation: RRID:Addgene_12817 Copy
Species: Homo sapiens
Genetic Insert: CCPG1
Vector Backbone Description: Backbone Marker:Dr.Shoji Yamaoka of Tokyo Medical and Dental University; Backbone Size:6389; Vector Backbone:pMRX-INU-FLAG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31006538
Proper citation: RRID:Addgene_128262 Copy
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