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Genetic Insert: rtTA-MAVS(C)
Vector Backbone Description: Backbone Size:7578; Vector Backbone:pLenti-CMVMCSSVBsd; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25746010
Proper citation: RRID:Addgene_65010 Copy
Genetic Insert: eyfp
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pUC18T-mini-Tn7T-Tp; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17406227
Comments: GenBank Accession # DQ493883
Proper citation: RRID:Addgene_65035 Copy
Species: Homo sapiens
Genetic Insert: Engineered target sites miR-148a-3p
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4637; Vector Backbone:pGL4.13; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24895996
Proper citation: RRID:Addgene_65053 Copy
Species: Homo sapiens
Genetic Insert: hsa-miR-148a (mutated)
Vector Backbone Description: Backbone Size:5175; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24895996
Comments: Please note that this plasmid was also described in PubMed ID 25714032.
Proper citation: RRID:Addgene_65055 Copy
Species: Homo sapiens
Genetic Insert: hsa-miR-216a (pre-miRNA)
Vector Backbone Description: Backbone Size:5175; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24895996
Proper citation: RRID:Addgene_65054 Copy
Species: Rattus norvegicus
Genetic Insert: DDIT4
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5500; Vector Backbone:pCMS-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17005863
Comments: There are 2 mutations if compared to PubMed DDIT4 R. Norvegicus sequence (L121V and S208G) because the insert was cloned out of DC12 cells DNA.
Proper citation: RRID:Addgene_65057 Copy
Species: Homo sapiens
Genetic Insert: Delta(Pro-MP)ADAM10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12743035
Comments: Mutagenesis creates EcoRI site 57bp downstream of Start ATG
Proper citation: RRID:Addgene_65107 Copy
Species: Rattus norvegicus
Genetic Insert: shNP1
Vector Backbone Description: Backbone Marker:Didier Trono; Backbone Size:11090; Vector Backbone:pLVTH; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25855168
Proper citation: RRID:Addgene_65062 Copy
Species: Rattus norvegicus
Genetic Insert: Neuronal Pentraxin 1
Vector Backbone Description: Backbone Marker:Didier Trono; Backbone Size:10510; Vector Backbone:pWPXL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25855168
Comments: The mutations introduce a new EcoR1 restriction site on bp 459 of CDS to allow distinction between wildtype and resistant NP1. However, this prevents excision of the whole resNP1 insert from the pWPXL vector using EcoR1 restriction site
Proper citation: RRID:Addgene_65061 Copy
Species: Rattus norvegicus
Genetic Insert: Neuronal Pentraxin 1
Vector Backbone Description: Backbone Marker:Didier Trono; Backbone Size:11078; Vector Backbone:pWPI; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25855168
Comments: * N280D and R322Q are present in NP1 and this plasmid should function as described in the associated publication.
Proper citation: RRID:Addgene_65060 Copy
Species: Synthetic
Genetic Insert: MS2
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pMal-c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18570876
Comments: The following reference describes how to isolate RNA binding complexes using this construct:
Jurica and Moore, Methods. 2002 Nov;28(3):336-45. https://www.ncbi.nlm.nih.gov/pubmed/12431437
Proper citation: RRID:Addgene_65104 Copy
Genetic Insert: ABKAR biosensor
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25892241
Proper citation: RRID:Addgene_65068 Copy
Genetic Insert: RBD, mCerulean, mVenus, RhoC
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5200; Vector Backbone:pTriEx; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24224016
Proper citation: RRID:Addgene_65071 Copy
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pPS854; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15908923
Comments: GenBank Accession # AY597269
Proper citation: RRID:Addgene_65009 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GRX2
Vector Backbone Description: Vector Backbone:p415TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23242256
Proper citation: RRID:Addgene_65005 Copy
Species: Mus musculus
Genetic Insert: Streptavidin-KDEL and truncated Mannosidase II
Vector Backbone Description: Backbone Marker:system biosciences; Vector Backbone:pCDH-CMV-MCS-EF1-puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22406856
Comments: luminal tagging
Proper citation: RRID:Addgene_65259 Copy
Species: Mus musculus
Genetic Insert: Streptavidin-KDEL and truncated Mannosidase II
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRES_neo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22406856
Comments: luminal tagging
Proper citation: RRID:Addgene_65253 Copy
Species: Mus musculus
Genetic Insert: Ii-Streptavidin and truncated Mannosidase II
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRESneo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22406856
Comments: luminal tagging
Proper citation: RRID:Addgene_65255 Copy
Species: Aequorea victoria
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3001; Vector Backbone:pGEM5Z(+); Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19248754
Comments: To be used in conjunction with pGEM5Z(+)-EGFP (p111): www.addgene.org/65206. See corrigendum/erratum for original publication (https://doi.org/10.1016/j.ab.2009.10.024) before performing experiments with this plasmid.
Proper citation: RRID:Addgene_65207 Copy
Species: Other
Genetic Insert: modified AAV-2 rep78
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5238; Vector Backbone:pFastBac-Dual; Vector Types:Insect Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19532142
Comments: Rep78 ORF utilizes a non-canonical initiation codon (CTG codon) beginning at nt 6822.
VP1 capsid ORF utilizes a non-canonical initiation codon (TTG codon) beginning at nt 6514.
Additional article references: Barbash et al. Gene Therapy 20: 274-282 (2013).
Proper citation: RRID:Addgene_65215 Copy
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