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Species: aquifex aeolicus
Genetic Insert: ultraID
Vector Backbone Description: Backbone Size:8488; Vector Backbone:pSF3; Vector Types:Mammalian Expression, Retroviral, Luciferase, to be used in a tet-on or tet-off compatible cell line; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35788163
Proper citation: RRID:Addgene_172878 Copy
Species: Synthetic
Genetic Insert: Ace2N-4AA-mNeon A122D
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37015225
Comments: Please visit https://doi.org/10.1101/2021.11.09.467909 for bioRxiv preprint.
Proper citation: RRID:Addgene_172911 Copy
Species: Synthetic
Genetic Insert: jGCaMP7s-T2A-tdTomato
Vector Backbone Description: Backbone Marker:Gerald Rubin; Vector Backbone:derived from pJFRC7; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_172916 Copy
Species: Mus musculus
Genetic Insert: Klf4
Vector Backbone Description: Backbone Marker:Olivier Destree; Backbone Size:5500; Vector Backbone:pGlomyc (based on pCDNA3.1); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.06.22.449461v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_172870 Copy
Vector Backbone Description: Backbone Marker:Opher Gileadi (Addgene plasmid 26106); Vector Backbone:pNH-TrxT (but TrxT removed); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34786571
Comments: pNH-TrxT that contains the sequence of Nanoluciferase in place of TrxT before the TEV cleavage site. Please visit https://www.biorxiv.org/content/10.1101/2021.05.31.445082v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_173075 Copy
Species: Prevotella pectinovora
Genetic Insert: pspCas13b
Vector Backbone Description: Backbone Size:9202; Vector Backbone:EF1a-lenti; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34257311
Proper citation: RRID:Addgene_173029 Copy
Species: Rattus norvegicus
Genetic Insert: PV-NES-GFP
Vector Backbone Description: Backbone Size:5900; Vector Backbone:pCMV-Myc-Cyto-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11971908
Proper citation: RRID:Addgene_17301 Copy
Species: Feline Immunodeficiency Virus
Genetic Insert: Gag-Pol and Rev
Vector Backbone Description: Backbone Size:12905; Vector Backbone:modified pUC119; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: This is the FIV PPR based second generation packaging vector, part of the FELIX vector system from Nolan lab. The FELIX vector system consists of three plasmids - structural, envelope, and transfer vector - which are cotransfected into 293T cells to produce virus. The structural construct makes Gag-Pol and Rev (some variants also make Vif and A). Any envelope construct can be used for pseudotyping - VSV-G tends to give the highest efficiency and broadest host range. The transfer vector contains the viral LTRs and packaging signal. Any expression cassette (promoter and gene of interest) can be cloned into the vector. Inserts can be up to 8.5kB. Please note that the sequence information has some inaccuracies, so restriction digests may give slightly different bands than predicted.
Proper citation: RRID:Addgene_1732 Copy
Genetic Insert: LacZ
Vector Backbone Description: Backbone Marker:Nolan Lab; Backbone Size:8396; Vector Backbone:pBMN; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Comments: This is Nolan lab's standard retrovirus vector backbone that expresses lacZ. Most of the other library vectors and IRES constructs are based on this expression vector. A variant comes with GFP in place of the lacZ gene (see pBMN-I-GFP).
Please note that there are some minor differences between Addgene's quality control sequence (see sequence with the pLXSN-5 primer) and the assembled sequence from the Nolan lab. See the following reference for further information: digitalcommons.unl.edu/cgi/viewcontent.cgi?article=1008&context=chemistrycerny
Proper citation: RRID:Addgene_1734 Copy
Species: Rattus norvegicus
Genetic Insert: Jagged1
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:6100; Vector Backbone:pIRES; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18077452
Comments: The backbone of this plasmid appears to be approximately 600bp smaller than is reported. The depositing scientist is aware of this difference and reports that the plasmid functions as it should.
Proper citation: RRID:Addgene_17336 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3287; Vector Backbone:pENTR4; Vector Types:RNAi, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17338 Copy
Species: Homo sapiens
Genetic Insert: eIF4E
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17289916
Comments: RT-PCR product of mRNA from human A549 cell.
Proper citation: RRID:Addgene_17343 Copy
Species: Homo sapiens
Genetic Insert: 4EHP
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17289916
Proper citation: RRID:Addgene_17344 Copy
Species: Streptomyces avidinii
Genetic Insert: streptavidin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4600; Vector Backbone:pET-3a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7499314
Proper citation: RRID:Addgene_17327 Copy
Species: Streptomyces avidinii
Genetic Insert: streptavidin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4600; Vector Backbone:pET-3a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11716692
Comments: A PCR-based site-directed mutagenesis method was used to modify the coding sequence of pTSA-13. A DNA sequence encoding six amino acid residues with a translation termination codon, GGT GGT TCT GGT TGC CCG TAG (Gly-Gly-Ser-Gly-Cys-Pro-Stop), was fused to the codon for Val-133 (GTG). The entire coding sequence was cloned between the NdeI and BamHI sites of the plasmid pET-3a under the control of the promoter of bacteriophage T7.
Proper citation: RRID:Addgene_17329 Copy
Genetic Insert: mNeonGreen-CBP-2XFLAG-HYGB
Vector Backbone Description: Backbone Size:4887; Vector Backbone:pRS316; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34791226
Proper citation: RRID:Addgene_173442 Copy
Species: Homo sapiens
Genetic Insert: Calcineurin Like EF-Hand Protein 1
Vector Backbone Description: Backbone Marker:Cell Biolabs; Backbone Size:5600; Vector Backbone:pMXS-IRES-Blast; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30846317
Proper citation: RRID:Addgene_173166 Copy
Genetic Insert: mNeonGreen-CBP-2XFLAG-NAT1
Vector Backbone Description: Backbone Size:4887; Vector Backbone:pRS316; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34791226
Proper citation: RRID:Addgene_173441 Copy
Species: Homo sapiens
Genetic Insert: 4EHP
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFlag-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17289916
Proper citation: RRID:Addgene_17348 Copy
Species: Synthetic
Genetic Insert: spNW50
Vector Backbone Description: Vector Backbone:pet28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34521837
Proper citation: RRID:Addgene_173486 Copy
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