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Species: Aequorea victora (modified) / synthetic
Genetic Insert: 7xTcf-eGFP // SV40-mCherry
Vector Backbone Description: Backbone Marker:Naldini/Trono (Addgene #12252); Backbone Size:6132; Vector Backbone:pRRLSIN.cPPT.PGK-GFP.WPRE ; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20186325
Comments: TCF repeat sequence is AGATCAAAGG
This plasmid requires a 2nd generation lentiviral packaging system.
Proper citation: RRID:Addgene_24304 Copy
Species: Aequorea victoria (modified) / Streptomyces alboniger
Genetic Insert: 7xTcf-eGFP // SV40-PuroR
Vector Backbone Description: Backbone Marker:Naldini/Trono (Addgene #12252); Backbone Size:6132; Vector Backbone:pRRLSIN.cPPT.PGK-GFP.WPRE ; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20186325
Comments: TCF repeat sequence is AGATCAAAGGgg.
This plasmid requires a 2nd generation lentiviral packaging system.
Proper citation: RRID:Addgene_24305 Copy
Species: Homo sapiens
Genetic Insert: Beclin1
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pCDNA4/TO-3*flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19050071
Proper citation: RRID:Addgene_24389 Copy
Species: Homo sapiens
Genetic Insert: CLASP2 (340-1084) 9xS/A
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:7469; Vector Backbone:pShuttle-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19289791
Comments: See the attached file for the old NIH reference sequence. Numbering starts at amino acid 78 and is off by 8 amino acids because of an insertion that is not accounted for.
Proper citation: RRID:Addgene_24384 Copy
Genetic Insert: mCD8-GFP
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pUAS-mCD8-GFP; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: The FRT-stop-FRT cassette, with additional BglII/NotI restriction sites, was PCR amplified from genomic DNA of UAS-FRT-stop-FRT-shibire (ts) flies and cloned into pUAST-mCD8-gfp to generate pUAST-FRT-stop-FRT-mCD8-gfp (pUAS>stop>mCD8-GFP)
Proper citation: RRID:Addgene_24385 Copy
Species: Homo sapiens
Genetic Insert: AGO4
Vector Backbone Description: Backbone Size:4969; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19946268
Proper citation: RRID:Addgene_24319 Copy
Species: Homo sapiens
Genetic Insert: human AGO2
Vector Backbone Description: Backbone Size:4969; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19946268
Comments: *Note that though the depositor lists XhoI as the 5' cloning enzyme, the AGO2 gene has an internal XhoI site. Addgene's quality control sequence shows that there is no intact XhoI site at the 5' end of the insert.
Proper citation: RRID:Addgene_24317 Copy
Species: Mus musculus
Genetic Insert: EF1alpha-betacatenin(deltaGSK) // SV40-PuroR
Vector Backbone Description: Backbone Marker:Naldini/Trono (Addgene #12252); Backbone Size:6074; Vector Backbone:pRRLSIN.cPPT.PGK-GFP.WPRE ; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20186325
Comments: This plasmid requires a 2nd generation lentiviral packaging system.
Proper citation: RRID:Addgene_24313 Copy
Species: Homo sapiens
Genetic Insert: Barkor
Vector Backbone Description: Backbone Size:5489; Vector Backbone:pCDNA3.0/Myc/His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19050071
Proper citation: RRID:Addgene_24394 Copy
Species: Homo sapiens
Genetic Insert: Barkor
Vector Backbone Description: Backbone Size:5489; Vector Backbone:pCDNA3.0/Myc/His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19050071
Proper citation: RRID:Addgene_24395 Copy
Species: Homo sapiens
Genetic Insert: Barkor
Vector Backbone Description: Backbone Size:5489; Vector Backbone:pCDNA3.0/Myc/His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19050071
Proper citation: RRID:Addgene_24396 Copy
Species: Homo sapiens
Genetic Insert: Beclin1
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pCDNA4/TO-3*flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19050071
Proper citation: RRID:Addgene_24390 Copy
Species: Homo sapiens
Genetic Insert: Beclin1
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pCDNA4/TO-3*flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19050071
Proper citation: RRID:Addgene_24393 Copy
Species: Homo sapiens
Genetic Insert: TRPC1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9215637
Proper citation: RRID:Addgene_24408 Copy
Species: Homo sapiens
Genetic Insert: CLASP2 (512-650)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19289791
Comments: See the attached file for the old NIH reference sequence. Numbering starts at amino acid 78 and is off by 8 amino acids because of an insertion that is not accounted for.
Proper citation: RRID:Addgene_24409 Copy
Species: Neurospora crassa
Genetic Insert: QF
Vector Backbone Description: Backbone Size:8300; Vector Backbone:pattB-DSCP-sv40; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: A newer version of this plasmid is now available - pattB-DSCP-QF#7-hsp70 www.addgene.org/46133 (Addgene plasmid # 46133)
Proper citation: RRID:Addgene_24369 Copy
Species: Homo sapiens
Genetic Insert: Barkor
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19050071
Proper citation: RRID:Addgene_24402 Copy
Species: Homo sapiens
Genetic Insert: Beclin1
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19050071
Proper citation: RRID:Addgene_24403 Copy
Species: Homo sapiens
Genetic Insert: Beclin1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pDsRed-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19050071
Proper citation: RRID:Addgene_24405 Copy
Species: Neurospora crassa
Genetic Insert: QS
Vector Backbone Description: Backbone Marker:Bischof et al., 2007; Backbone Size:9000; Vector Backbone:pUAST attB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: The QS cDNA was excised from pAC-QS with Acc65I and NotI, blunted, and ligated
into a blunted pUASTattB (Bischof et al., 2007) which had been digested with BglII and KpnI.
Proper citation: RRID:Addgene_24366 Copy
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