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Species: Synthetic
Genetic Insert: proD guide
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_65820 Copy
Genetic Insert: RhlR
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26315440
Comments: Integrated into the attP site
Proper citation: RRID:Addgene_65954 Copy
Species: Sulfolobus solfataricus P2
Genetic Insert: DNA polymerase sliding clamp 1
Vector Backbone Description: Backbone Marker:TaKaRa; Backbone Size:2206; Vector Backbone:pHSG398; Vector Types:Cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20607777
Comments: We cloned the PCNA1 gene from the cell suspension of S. solfataricus P2 strain (ATCC35092).
Proper citation: RRID:Addgene_66125 Copy
Species: Other
Genetic Insert: STp
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5996; Vector Backbone:pBR325; Vector Types:cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:3284902
Comments: Contains the E. coli STp gene probe (157 bp) cloned on PstI linkers into pBR325 Heat stable enterotoxin gene (porcine)
Proper citation: RRID:Addgene_66149 Copy
Species: Synechocystis sp. 6803
Genetic Insert: CcaS
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:1900; Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24608181
Comments: Note that Addgene NGS identified a nonsense mutation in the CcaS insert
Proper citation: RRID:Addgene_50548 Copy
Species: Synthetic
Genetic Insert: Cph8
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:1995; Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24608181
Comments: Encodes the Cph8 (Cph1/EnvZ fusion) light sensor kinase/phosphatase downstream of the pLTetO-1 promoter.
Requires pPLPCB(S)t or some other source of TetR
Requires PCB to function
Does NOT include the response regulator.
Proper citation: RRID:Addgene_50552 Copy
Species: Synechocystis sp. 6803
Genetic Insert: CcaS
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:1900; Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24608181
Proper citation: RRID:Addgene_50551 Copy
Species: Synthetic
Genetic Insert: T1, gRNA scaffold, OsU3t plus, TaU3p, T2
Vector Backbone Description: Vector Backbone:pGWC; Vector Types:Plant Expression, CRISPR, PCR template; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25432517
Comments: Please note that this plasmid runs as a dimer (>7kb) or multimer.
Proper citation: RRID:Addgene_50593 Copy
Species: Synthetic
Genetic Insert: T3, gRNA scaffold, U6-26t plus, OsU3p, T4
Vector Backbone Description: Vector Backbone:pGWC; Vector Types:Plant Expression, CRISPR, PCR template; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25432517
Proper citation: RRID:Addgene_50595 Copy
Species: Synthetic
Genetic Insert: T1, U626t plus, U629p, T2
Vector Backbone Description: Vector Backbone:pGWC; Vector Types:Plant Expression, CRISPR, PCR template; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25432517
Proper citation: RRID:Addgene_50590 Copy
Species: Synthetic
Genetic Insert: T3, gRNA scaffold, U6-1tplus, U6-26p, T4
Vector Backbone Description: Vector Backbone:pGWC; Vector Types:Plant Expression, CRISPR, PCR template; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25432517
Proper citation: RRID:Addgene_50592 Copy
Vector Backbone Description: Vector Backbone:pCC1BAC; Vector Types:Synthetic Biology, Episomal vector for Phaeodactylum tricornutum; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25897682
Comments: Contains yeast URA3 and HIS3 genes for selection in yeast. Contains ShBle cassette for selection in Phaeodactylum tricornutum.
Depositor states that the I-SceI restriction site in this plasmid (5’-AGTTACGCTAGGGATAACAGGTAATATAG) varies from the NEB listed recognition sequence and may reduce the efficiency of cleavage using this enzyme.
Proper citation: RRID:Addgene_62862 Copy
Vector Backbone Description: Backbone Marker:BioBrick ; Backbone Size:4100; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25756291
Proper citation: RRID:Addgene_63199 Copy
Species: Synthetic
Genetic Insert: RFP cloning selection cassette
Vector Backbone Description: Backbone Marker:BioBricks Foundation; Backbone Size:2069; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26171760
Proper citation: RRID:Addgene_63674 Copy
Species: Other
Genetic Insert: LT gene probe
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:2254425
Comments: The LT probe is a 1-kilobase BamHI fragment derived from pWD299. The HincII fragment was removed from pWD299, BamHI linkers were attached, and this fragment was cloned into pACYC184
Proper citation: RRID:Addgene_63740 Copy
Species: Enteropathogenic Escherichia coli
Genetic Insert: eae
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:2172966
Proper citation: RRID:Addgene_63759 Copy
Species: Rhodobactor
Genetic Insert: crtEBI operon
Vector Backbone Description: Vector Backbone:FTV; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24952589
Comments: Needs to be grown in E_IF3_15 strain for overproduction of neurosporene
Proper citation: RRID:Addgene_63803 Copy
Species: Rhodobactor
Genetic Insert: crtEBI operon
Vector Backbone Description: Vector Backbone:FTV; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24952589
Comments: Needs to be grown in E_IF3_15 strain for overproduction of neurosporene
Proper citation: RRID:Addgene_63804 Copy
Species: Rhodobactor
Genetic Insert: crtEBI operon
Vector Backbone Description: Vector Backbone:FTV; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24952589
Comments: Needs to be grown in E_IF3_15 strain for overproduction of neurosporene
Proper citation: RRID:Addgene_63806 Copy
Species: Rhodobactor
Genetic Insert: crtEBI operon
Vector Backbone Description: Vector Backbone:FTV; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24952589
Comments: Needs to be grown in E_IF3_15 strain for overproduction of neurosporene
Proper citation: RRID:Addgene_63812 Copy
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