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  • Bacterial Resistance:ampicillin and kanamycin (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
LSB-hsa-miR-766-5p
 
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RRID:Addgene_103732 hsa-miR-766-5p target Homo sapiens Ampicillin and Kanamycin PMID:29934631 Please note that this plasmid is inherently unstable due to a repeating poly A element. We recommend performing a diagnostic digest on multiple colonies upon receipt. The depositors would like to note the presence of several discrepancies between the depositor sequence and physical plasmid sequence. In particular, the sequences upstream and downstream of the puromycin coding region have 10 bp insertions which do not affect the ability to perform puromycin selection. The sequences for those regions within the physical plasmids are as follows: Upstream of Puro: GAATTCGACCGCCTGTCTCAAGGTGCCACC; Downstream of Puro: GCTTTGAGACTACGCGTGAATTCACTCCTC. Please contact [email protected] if you are interested in this plasmid prior to placing an order. Vector Backbone:Low Sensor Backbone (LSB); Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:00:35 0
LSB-hsa-miR-887-3p
 
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RRID:Addgene_103746 hsa-miR-887-3p target Homo sapiens Ampicillin and Kanamycin PMID:29934631 Please note that this plasmid is inherently unstable due to a repeating poly A element. We recommend performing a diagnostic digest on multiple colonies upon receipt. The depositors would like to note the presence of several discrepancies between the depositor sequence and physical plasmid sequence. In particular, the sequences upstream and downstream of the puromycin coding region have 10 bp insertions which do not affect the ability to perform puromycin selection. The sequences for those regions within the physical plasmids are as follows: Upstream of Puro: GAATTCGACCGCCTGTCTCAAGGTGCCACC; Downstream of Puro: GCTTTGAGACTACGCGTGAATTCACTCCTC. Please contact [email protected] if you are interested in this plasmid prior to placing an order. Vector Backbone:Low Sensor Backbone (LSB); Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:00:35 0
LSB-hsa-miR-885-3p
 
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RRID:Addgene_103744 hsa-miR-885-3p target Homo sapiens Ampicillin and Kanamycin PMID:29934631 Please note that this plasmid is inherently unstable due to a repeating poly A element. We recommend performing a diagnostic digest on multiple colonies upon receipt. The depositors would like to note the presence of several discrepancies between the depositor sequence and physical plasmid sequence. In particular, the sequences upstream and downstream of the puromycin coding region have 10 bp insertions which do not affect the ability to perform puromycin selection. The sequences for those regions within the physical plasmids are as follows: Upstream of Puro: GAATTCGACCGCCTGTCTCAAGGTGCCACC; Downstream of Puro: GCTTTGAGACTACGCGTGAATTCACTCCTC. Please contact [email protected] if you are interested in this plasmid prior to placing an order. Vector Backbone:Low Sensor Backbone (LSB); Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:00:35 0
LSB-hsa-miR-708-3p
 
Resource Report
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RRID:Addgene_103724 hsa-miR-708-3p target Homo sapiens Ampicillin and Kanamycin PMID:29934631 Please note that this plasmid is inherently unstable due to a repeating poly A element. We recommend performing a diagnostic digest on multiple colonies upon receipt. The depositors would like to note the presence of several discrepancies between the depositor sequence and physical plasmid sequence. In particular, the sequences upstream and downstream of the puromycin coding region have 10 bp insertions which do not affect the ability to perform puromycin selection. The sequences for those regions within the physical plasmids are as follows: Upstream of Puro: GAATTCGACCGCCTGTCTCAAGGTGCCACC; Downstream of Puro: GCTTTGAGACTACGCGTGAATTCACTCCTC. Please contact [email protected] if you are interested in this plasmid prior to placing an order. Vector Backbone:Low Sensor Backbone (LSB); Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:00:35 0
LSB-hsa-miR-7-5p
 
Resource Report
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RRID:Addgene_103723 hsa-miR-7-5p target Homo sapiens Ampicillin and Kanamycin PMID:29934631 Please note that this plasmid is inherently unstable due to a repeating poly A element. We recommend performing a diagnostic digest on multiple colonies upon receipt. The depositors would like to note the presence of several discrepancies between the depositor sequence and physical plasmid sequence. In particular, the sequences upstream and downstream of the puromycin coding region have 10 bp insertions which do not affect the ability to perform puromycin selection. The sequences for those regions within the physical plasmids are as follows: Upstream of Puro: GAATTCGACCGCCTGTCTCAAGGTGCCACC; Downstream of Puro: GCTTTGAGACTACGCGTGAATTCACTCCTC. Please contact [email protected] if you are interested in this plasmid prior to placing an order. Vector Backbone:Low Sensor Backbone (LSB); Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:00:37 0
pCR4-TOPO_L31-FLAG-TEV-AviTag_repair_template
 
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RRID:Addgene_235608 Rpl31 C-terminus with FLAG-TEV-AviTag and flanking homology arms Synthetic Ampicillin and Kanamycin PMID:40048539 Backbone Marker:Invitrogen; Vector Backbone:pCR-4Blunt-TOPO; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:32:31 0
pTn Donor
 
Resource Report
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1+ mentions
RRID:Addgene_236211 None Ampicillin and Kanamycin PMID:40148565 Please visit https://doi.org/10.1101/2024.05.21.595064 for bioRxiv preprint. Vector Backbone:R6K origin; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:32:31 1
pYJ01-Guide-SOX17-E1-gRNA1
 
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RRID:Addgene_131046 SOX17 Exon1 gRNA1 Homo sapiens Ampicillin and Kanamycin Backbone Size:3979; Vector Backbone:pGuide-U6-gRNA; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:34:04 0
pcRII-mogat3a in situ probe
 
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RRID:Addgene_232135 mogat3a Danio rerio Ampicillin and Kanamycin PMID:39510175 Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCRII-TOPO; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:31:47 0
pCRII-TOPO-mogat2 in situ probe
 
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RRID:Addgene_232139 mogat2 Danio rerio Ampicillin and Kanamycin PMID:39510175 Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCRII-TOPO; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:31:47 0
pCRII-TOPO-dgat1b in situ probe
 
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RRID:Addgene_232138 dgat1b Danio rerio Ampicillin and Kanamycin PMID:39510175 Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCRII-TOPO; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:31:47 0
pTA-attB
 
Resource Report
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1+ mentions
RRID:Addgene_18937 phiC31 attB Saccharomyces cerevisiae Ampicillin and Kanamycin PMID:10801973 Backbone Marker:Invitrogen; Backbone Size:3805; Vector Backbone:pCR2.1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:11:40 1
pSEVA12DKR-J
 
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RRID:Addgene_58306 Genomic cassette Synthetic Ampicillin and Kanamycin PMID:24847673 Vector Backbone:SEVA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:17:15 0
pSEVA12TEC-I
 
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RRID:Addgene_58308 Genomic cassette Synthetic Ampicillin and Kanamycin PMID:24847673 Please note that Addgene's NGS found a 1209bp transposase insertion between the RepA and Epsilon Antitoxin sequence. The effect of this insertion is unknown. If you are interested in the version without the insertion, please contact the depositing lab (Tom Ellis lab). Vector Backbone:SEVA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:17:15 0
pZmU3-gRNA
 
Resource Report
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1+ mentions
RRID:Addgene_53061 zmU3 promoter - gRNA maize (corn) Ampicillin and Kanamycin PMID:24576457 Backbone Marker:Transgen; Backbone Size:3929; Vector Backbone:pEasy-blunt; Vector Types:CRISPR, Plant expression; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:16:31 1
pIKM1
 
Resource Report
Resource Website
RRID:Addgene_51054 Ampicillin and Kanamycin PMID:14766568 For more information about this plasmid, see the associated publication (PMID: 14766568), as well as the publication describing its original construction: Mai, Lorenz, and Wiegel, Transformation of Thermoanaerobacterium sp. strain JW/SL-YS485 with plasmid pIKM1 conferring kanamycin resistance. FEMS Microbiol Lett. 1997 DOI: 10.1111/j.1574-6968.1997.tb10283.x ( http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.1997.tb10283.x/abstract ) Backbone Marker:Mermelstein et al. 1992; Backbone Size:4500; Vector Backbone:pIMP1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:16:13 0
p2attNG-H11-short
 
Resource Report
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1+ mentions
RRID:Addgene_51546 GFP4m Ampicillin and Kanamycin PMID:24304893 Vector Backbone:p2attNG; Vector Types:Mammalian Expression, Human Targeting; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:16:14 1
mCherryS131C
 
Resource Report
Resource Website
RRID:Addgene_48732 mCherryS131C Synthetic Ampicillin and Kanamycin PMID:21696135 This plasmid must be grown under Ampicillin (100ug/ml) and Kanamycin (50ug/ml) selection in order to retain both the mCherryS131C plasmid and the pREP4 helper plasmid. Please note that verifying this plasmid by sequencing or digest is challenging due to the presence of the helper plasmid, as multiple bands or mispriming is likely to occur. See Addgene's sequencing results for recommended primers. The S131C mutation in this plasmid is S136C, when compared to the starting Met of wild-type mCherry. Backbone Marker:QIAGEN; Backbone Size:3439; Vector Backbone:pQE-9; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin changed serine 131 to cysteine 2026-08-15 01:15:53 0
pJC-lecg
 
Resource Report
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RRID:Addgene_239180 lecg Schmidtea mediterranea Ampicillin and Kanamycin PMID:35294875 dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single-stranded probes can be generated with SP6 or T3 RNA polymerases Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:33:08 0
Culex-027
 
Resource Report
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RRID:Addgene_245842 16S rRNA Culexarchaeum yellowstonense LCB-24-027 Ampicillin and Kanamycin PMID:41247030 Vector Backbone:pcr2.1-oriT; Vector Types:Other; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:34:21 0

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