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Species: Danio rerio
Genetic Insert: Kitlgb
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, Vertebrate expression (zebrafish, chicken, xenopus); Bacterial Resistance:Ampicillin
Defining Citation: PMID:33259600
Comments: Please note I85V, V222I, and D233N are a common SNP and does not affect plasmid function.
Proper citation: RRID:Addgene_168200 Copy
Species: Danio rerio
Genetic Insert: Thrombopoietin
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, Vertebrate expression (zebrafish, chicken, xenopus); Bacterial Resistance:Ampicillin
Defining Citation: PMID:24869937
Proper citation: RRID:Addgene_168198 Copy
Species: Danio rerio
Genetic Insert: Kitlga
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, Vertebrate expression (zebrafish, chicken, xenopus); Bacterial Resistance:Ampicillin
Defining Citation: PMID:33259600
Comments: Please note F198L is a common SNP and does not affect plasmid function.
Proper citation: RRID:Addgene_168199 Copy
Species: Danio rerio
Genetic Insert: lef1
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Zebrafish Lef-1 in unknown vector for making antisense probe. From Chris.
For antisense: EcoRI, T7.
Proper citation: RRID:Addgene_16885 Copy
Species: Danio rerio
Genetic Insert: isthmin
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSPORT1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15797017
Comments: Zebrafish Isthmin cDNA, clone 27j15 from the microarray screen 1st round (Matthew Clark normalized late somitogenesis / 24 hpf / liver library) in pSPORT1. A clone that is slightly longer at the 5' end was identified in the 2nd array round, so this is not exactly full-length. For antisense probe synthesis.
Expressed on dorsal side early, in a dorsal high to ventral low gradient in margin, during gastrulation around axial mesoderm, later strongly at MHB. For antisense: Asp718, SP6.
Citation: G. Weidinger and R. T. Moon (2005). Development.
Proper citation: RRID:Addgene_16881 Copy
Species: Danio rerio
Genetic Insert: cystathionine beta-synthase
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSPORT1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15797017
Comments: Zebrafish cystathionine beta-synthase cDNA (likely full-lenghth, at least contains full coding sequence) in pSPORT1. clone 6h9 from the microarray screen 1st round (Matthew clark normalized late somitogenesis / 24 hpf / liver library).
Expressed pretty ubiquitously early, but stronger in axail mesoderm during gastrulation. Later most prominently in eyes and somites.
For antisense: Asp718, SP6.
Citation: G. Weidinger et al Development 2005.
Proper citation: RRID:Addgene_16880 Copy
Species: Danio rerio
Genetic Insert: wnt8 3' orf1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T easy; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11703928
Comments: 530 bp PCR product ligated into vector pGEMTeasy. Purpose of clone is to provide an in situ probe specific for the first open reading frame of the bicistronic wnt8 RNA.
To make antisense probe: cut with Sph1, transcribe with SP6.
Citation: A. Lekven et al., 2001, Developmental Cell.
Proper citation: RRID:Addgene_16899 Copy
Species: Danio rerio
Genetic Insert: Nlk1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15151990
Comments: For antisense probe cut with SacII and transcribe with SP6. For sense probe cut with Sal1 and transcribe with T7.
Citation: C. J. Thorpe and R. T. Moon (2004). Development 131:2899-2909.
Proper citation: RRID:Addgene_16895 Copy
Species: Danio rerio
Genetic Insert: zvox-1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Zvox-1 in CS2+. EcoRI-HpaI fragment from Zvox-1-2 was inserted into KS EcoRI-Sma as ZV80. EcoRI-XbaI fragment from this was inserted into CS2+ EcoRI-XbaI.
To make RNA: cut with Asp718, transcribe with SP6.
Constructed by: David Kimelman, 2/1/97.
Proper citation: RRID:Addgene_16900 Copy
Species: Danio rerio
Genetic Insert: pax2
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: For probe digest with EcoRI, transcribe with SP6 polymerase.
Proper citation: RRID:Addgene_16906 Copy
Species: Danio rerio
Genetic Insert: tbx5
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Details unknown.
Proper citation: RRID:Addgene_16905 Copy
Species: Danio rerio
Genetic Insert: fgf8
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Fgf8 insert (XhoI-ApaI) from fgf8-CS2 cloned into XhoI-ApaI cut pSK+.
For antisense probe digest with XhoI, transcribe with T7 polymerase.
Proper citation: RRID:Addgene_16912 Copy
Species: Danio rerio
Genetic Insert: Mnx1 enhancer (3x)
Vector Backbone Description: Backbone Marker:Chi-Bin Chien lab; Backbone Size:2663; Vector Backbone:p5E; Vector Types:Gateway Entry Cloning Vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32516597
Comments: See the following paper for cloning details of the 3x 125bp mnx1 enhancer: Zelenchuk, T.A. and Brusés, J.L. (2011), In Vivo labeling of zebrafish motor neurons using an mnx1 enhancer and Gal4/UAS. Genesis, 49: 546-554. https://doi.org/10.1002/dvg.20766
Proper citation: RRID:Addgene_169043 Copy
Species: Danio rerio
Genetic Insert: wnt-1
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: cDNA clone of wnt-1 for DIG labelled probes cloned by G.
Kelly. Antisense: linearize with EcoR1 and transcribe with SP6 RNA polyermase. Sense: linearize with HindIII and transcribe with T7 RNA polymerase. Hydrolyze probes for 12 minutes according to Harland, 1991.
Proper citation: RRID:Addgene_16925 Copy
Species: Danio rerio
Genetic Insert: Krox20
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Linearize with Xba1 and transcribe with T3 for antisense in situ probe.
Proper citation: RRID:Addgene_16924 Copy
Species: Danio rerio
Genetic Insert: Cavin4b
Vector Backbone Description: Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34633413
Proper citation: RRID:Addgene_176011 Copy
Species: Danio rerio
Genetic Insert: Bin1b
Vector Backbone Description: Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34633413
Proper citation: RRID:Addgene_176014 Copy
Species: Danio rerio
Genetic Insert: Cavin4a
Vector Backbone Description: Vector Backbone:SITS-ccdb-EGFP (Addgene 118752); Vector Types:Leishmania cell free expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34633413
Proper citation: RRID:Addgene_176018 Copy
Species: Danio rerio
Genetic Insert: Cavin4b
Vector Backbone Description: Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34633413
Proper citation: RRID:Addgene_176009 Copy
Species: Danio rerio
Genetic Insert: Cavin4b
Vector Backbone Description: Vector Backbone:SITS-ccdb-mCherry (Addgene 118753); Vector Types:Leishmania cell free expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34633413
Proper citation: RRID:Addgene_176023 Copy
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