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Species: Other
Genetic Insert: 6His-TEV-TBP6.7(R47A)
Vector Backbone Description: Backbone Size:5277; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29961805
Proper citation: RRID:Addgene_112722 Copy
Species: Other
Genetic Insert: 6His-TEV-TBP6.7(R49A)
Vector Backbone Description: Backbone Size:5277; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29961805
Proper citation: RRID:Addgene_112726 Copy
Species: Other
Genetic Insert: uvrA-like protein
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28017602
Comments: uvrA gene was taken from the Echinomycin biosynthetic gene cluster. JoVE antibiotic dereplication paper: Zubyk, H.L., Cox, G., Wright, G.D. Antibiotic Dereplication Using the Antibiotic Resistance Platform. J. Vis. Exp. (152), e60536, doi:10.3791/60536 (2019). (https://www.jove.com/video/60536/antibiotic-dereplication-using-the-antibiotic-resistance-platform)
Proper citation: RRID:Addgene_112892 Copy
Species: Other
Genetic Insert: fosA
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28017602
Comments: Please note that some discrepancies were found between Addgene's quality control and the depositor's insert sequence. The depositor noted that these discrepancies do NOT affect plasmid function. JoVE antibiotic dereplication paper: Zubyk, H.L., Cox, G., Wright, G.D. Antibiotic Dereplication Using the Antibiotic Resistance Platform. J. Vis. Exp. (152), e60536, doi:10.3791/60536 (2019). (https://www.jove.com/video/60536/antibiotic-dereplication-using-the-antibiotic-resistance-platform)
Proper citation: RRID:Addgene_112889 Copy
Species: Other
Genetic Insert: eSpCas9
Vector Backbone Description: Vector Backbone:N.A.; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29982721
Comments: Please visit https://www.biorxiv.org/content/early/2018/02/26/272377 for bioRxiv preprint.
Proper citation: RRID:Addgene_113152 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:N.A.; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29982721
Comments: Please visit https://www.biorxiv.org/content/early/2018/02/26/272377 for bioRxiv preprint.
Proper citation: RRID:Addgene_113150 Copy
Species: Other
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:N.A.; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29946130
Comments: Please visit https://www.biorxiv.org/content/early/2018/02/26/272377 for bioRxiv preprint.
Proper citation: RRID:Addgene_113149 Copy
Species: Other
Genetic Insert: msfGFP
Vector Backbone Description: Vector Backbone:pFNMB1; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30234022
Proper citation: RRID:Addgene_113191 Copy
Species: Other
Genetic Insert: dCas9, MCP-TetD, J107 scRNA.b1_MS2
Vector Backbone Description: Vector Backbone:p15A vector; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29950558
Proper citation: RRID:Addgene_113320 Copy
Species: Other
Genetic Insert: dCas9 and MCP-SoxS_R93A
Vector Backbone Description: Vector Backbone:p15A vector; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29950558
Comments: Plasmid contains a G nucleotide deletion in the BBa_B1002 terminator sequence, which is not known to affect plasmid function.
Proper citation: RRID:Addgene_113324 Copy
Species: Other
Genetic Insert: TfRΔEctoR1-mRFP-GFPnb
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29590595
Proper citation: RRID:Addgene_113555 Copy
Species: Other
Genetic Insert: Flag
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:5344; Vector Backbone:pcDNA5/FRT/TO/intron; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29425494
Proper citation: RRID:Addgene_113548 Copy
Species: Other
Genetic Insert: ndmC
Vector Backbone Description: Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31540989
Comments: https://benchling.com/s/COGhnD32 . Please visit https://www.biorxiv.org/content/early/2018/09/16/419283 for bioRxiv preprint.
Proper citation: RRID:Addgene_113648 Copy
Species: Other
Genetic Insert: ndmB
Vector Backbone Description: Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31540989
Comments: https://benchling.com/s/2C3Oh1ew . Please visit https://www.biorxiv.org/content/early/2018/09/16/419283 for bioRxiv preprint.
Proper citation: RRID:Addgene_113647 Copy
Species: Other
Genetic Insert: ndmA
Vector Backbone Description: Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31540989
Comments: https://benchling.com/s/4vRm4arA . Please visit https://www.biorxiv.org/content/early/2018/09/16/419283 for bioRxiv preprint.
Proper citation: RRID:Addgene_113646 Copy
Species: Other
Genetic Insert: mKaxxte2.5
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30804405
Comments: One clones a fragment of DNA sequence at the MCS, then uses a targeted nuclease, such as Cas9 to trigger a double-strand break. This is then repaired by homologous recombination resulting in restoration of an intact mKate2.5 fluorescent protein (cloned from Addgene #54828). It can be used to test Cas9 gRNA efficiency, as it was in the paper, or HR-capacity in different cells.
Proper citation: RRID:Addgene_113630 Copy
Species: Other
Genetic Insert: ndmB and ndmC
Vector Backbone Description: Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31540989
Comments: https://benchling.com/s/kNCaeGXO . Please visit https://www.biorxiv.org/content/early/2018/09/16/419283 for bioRxiv preprint.
Proper citation: RRID:Addgene_113651 Copy
Species: Other
Genetic Insert: promoter T5 and sgRNA
Vector Backbone Description: Backbone Size:2906; Vector Backbone:pYTK095; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30703321
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/16/419283 for bioRxiv preprint.
Proper citation: RRID:Addgene_113655 Copy
Species: Other
Genetic Insert: promoter J23119 and sgRNA
Vector Backbone Description: Backbone Size:2906; Vector Backbone:pYTK095; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30703321
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/16/419283 for bioRxiv preprint.
Proper citation: RRID:Addgene_113654 Copy
Species: Other
Genetic Insert: ndmA and ndmB and ndmC
Vector Backbone Description: Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31540989
Comments: https://benchling.com/s/xeoN8t6c . Please visit https://www.biorxiv.org/content/early/2018/09/16/419283 for bioRxiv preprint.
Proper citation: RRID:Addgene_113652 Copy
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