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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pPEE20 Resource Report Resource Website |
RRID:Addgene_128363 | Acetamidase (amdS) | Synthetic | Ampicillin | PMID:31220607 | Contains the Safe Haven Site 4 sequence (CNAG_06526 - CNAG_06527) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site. | Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:36 | 0 | |
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pPEE19 Resource Report Resource Website |
RRID:Addgene_128362 | Acetamidase (amdS) | Synthetic | Ampicillin | PMID:31220607 | Contains the Safe Haven Site 3 sequence (CNAG_02589 - CNAG_02588) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site. | Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:36 | 0 | |
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p2HGPD/ER/cyc Resource Report Resource Website |
RRID:Addgene_1283 | ER | Saccharomyces cerevisiae | Ampicillin | PMID:7761857 | Backbone Size:0; Vector Backbone:2u (HIS3); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:36 | 0 | ||
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pPEE17 Resource Report Resource Website |
RRID:Addgene_128361 | Acetamidase (amdS) | Synthetic | Ampicillin | PMID:31220607 | Contains the Safe Haven Site 1 sequence (CNAG_00777 - CNAG_00778) in the backbone of pBluescript II SK- flanked by blaster repeats to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site. | Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:36 | 0 | |
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pSDMA65 Resource Report Resource Website |
RRID:Addgene_128354 | Hygromycin (HYG) | Synthetic | Ampicillin | PMID:27711143 | Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:CRISPR; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:36 | 0 | ||
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pQCXIP-mCherry-TAZ Resource Report Resource Website |
RRID:Addgene_128351 | TAZ | Homo sapiens | Ampicillin | pCIneomCherry-TAZ was generated by ligating TAZ(EcoRI/XhoI) into EcoRI/Sall sites of pCIneomCherry. NheI/NotI fragment was ligated inot XbaI/NotI sites of pQCXIP. | Backbone Marker:Clontech; Vector Backbone:pQCXIP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:36 | 0 | ||
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pSDMA67 Resource Report Resource Website |
RRID:Addgene_128356 | Noureothricin (NAT) | Synthetic | Ampicillin | PMID:27711143 | Please note: The plasmid contains a 390bp discrepancy in C-terminus of the LacZ Split. This region of the plasmid serves no purpose other than for screening and hosting the MCS used in generating the plasmid. This difference is not expected to affect the final function of this plasmid. | Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:36 | 0 | |
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pSDMA66 Resource Report Resource Website |
RRID:Addgene_128355 | Noureothricin (NAT) | Synthetic | Ampicillin | PMID:27711143 | Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:36 | 0 | ||
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DCT.VV.Orf141 Resource Report Resource Website |
RRID:Addgene_12829 | VV.Orf141 | Vaccinia Virus | Ampicillin | PMID:15623576 | Please see the Vaccinia kit page ( https://www.addgene.org/vvorf/ ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid. | Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA 3.1D V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:36 | 0 | |
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pPEE13 Resource Report Resource Website |
RRID:Addgene_128359 | Acetamidase (amdS) | Synthetic | Ampicillin | PMID:31220607 | Contains the amdS2 mCherry Blaster marker with a flexable linker in the multicloning site of pBluescript II SK-. Importantly, the amdS2 marker can be excised using restriction enzymes HindIII and EcoRI. | Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:36 | 0 | |
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DCT.VV.Orf144 Resource Report Resource Website |
RRID:Addgene_12832 | VV.Orf144 | Vaccinia Virus | Ampicillin | PMID:15623576 | Please see the Vaccinia kit page ( https://www.addgene.org/vvorf/ ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid. | Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA 3.1D V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:36 | 0 | |
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DCT.VV.Orf145 Resource Report Resource Website |
RRID:Addgene_12833 | VV.Orf145 | Vaccinia Virus | Ampicillin | PMID:15623576 | Please see the Vaccinia kit page ( https://www.addgene.org/vvorf/ ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid. | Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA 3.1D V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:36 | 0 | |
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DCT.VV.Orf143 Resource Report Resource Website |
RRID:Addgene_12831 | VV.Orf143 | Vaccinia Virus | Ampicillin | PMID:15623576 | Please see the Vaccinia kit page ( https://www.addgene.org/vvorf/ ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid. | Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA 3.1D V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:36 | 0 | |
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pFB-U6-Chrna6-gRNA-hSyn-mCherry-WPRE-SV40pA Resource Report Resource Website |
RRID:Addgene_128341 | Chrna6 | Mus musculus | Ampicillin | PMID:29779223 | Backbone Marker:Keith Joung (Addgene plasmid #43860); Vector Backbone:MLM3636; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:36 | 0 | ||
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pFB-U6-sgNeuN-gRNA-hSyn-mCherry-WPRE-SV40pA Resource Report Resource Website |
RRID:Addgene_128346 | Rbfox3 (NeuN) | Mus musculus | Ampicillin | PMID:29779223 | Backbone Marker:Keith Joung (Addgene plasmid #43860); Vector Backbone:MLM3636; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:36 | 0 | ||
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pFB-U6-Chrnb3-gRNA-hSyn-mCherry-WPRE-SV40pA Resource Report Resource Website |
RRID:Addgene_128344 | Chrnb3 | Mus musculus | Ampicillin | PMID:29779223 | Backbone Marker:Keith Joung (Addgene plasmid #43860); Vector Backbone:MLM3636; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:36 | 0 | ||
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DCT.VV.Orf148 Resource Report Resource Website |
RRID:Addgene_12836 | VV.Orf148 | Vaccinia Virus | Ampicillin | PMID:15623576 | Please see the Vaccinia kit page ( https://www.addgene.org/vvorf/ ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid. | Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA 3.1D V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:36 | 0 | |
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DCT.VV.Orf146 Resource Report Resource Website |
RRID:Addgene_12834 | VV.Orf146 | Vaccinia Virus | Ampicillin | PMID:15623576 | Please see the Vaccinia kit page ( https://www.addgene.org/vvorf/ ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid. | Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA 3.1D V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:36 | 0 | |
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pSPB848 Resource Report Resource Website |
RRID:Addgene_128299 | Para - lp-parB | Ampicillin | PMID:31406375 | Cloning method: Golden Gate. | Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:36 | 0 | ||
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pSM040 Resource Report Resource Website |
RRID:Addgene_128294 | Piq - mRFP, parS | Chloramphenicol | PMID:31406375 | Cloning method: Golden Gate. | Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | Addgene | 2026-10-24 02:05:36 | 0 |
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