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Species: Mus musculus
Genetic Insert: Unc5c.z
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Comments: Unc5c.z is missing exon 3.
Proper citation: RRID:Addgene_72182 Copy
Species: Mus musculus
Genetic Insert: Ten-Eleven Translocation 2
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21778364
Comments: Modified pcDNA3 vector with a beta globulin intron inserted. This insertion can help stabilize the transcripts and is particularly useful for the expression of large genes, such as p300 and Tet proteins
For additional information about how this plasmid was created and used, please see Ito et al., Nature 2010 Pubmed ID: 20639862
Proper citation: RRID:Addgene_72220 Copy
Species: Mus musculus
Genetic Insert: Ten-Eleven Translocation 1
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21778364
Comments: Modified pcDNA3 vector with a beta globulin intron inserted. This insertion can help stabilize the transcripts and is particularly useful for the expression of large genes, such as p300 and Tet proteins.
Note that an additional mutation, K1581H, was introduced by PCR. This additional mutation does not affect the property of the mutant protein (as it is catalytically dead).
For additional information about how this plasmid was created and used, please see Ito et al., Nature 2010 Pubmed ID: 20639862
Proper citation: RRID:Addgene_72218 Copy
Species: Mus musculus
Genetic Insert: Ten-Eleven Translocation 2
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21778364
Comments: Modified pcDNA3 vector with a beta globulin intron inserted. This insertion can help stabilize the transcripts and is particularly useful for the expression of large genes, such as p300 and Tet proteins
For additional information about how this plasmid was created and used, please see Ito et al., Nature 2010 Pubmed ID: 20639862
Proper citation: RRID:Addgene_72219 Copy
Species: Mus musculus
Genetic Insert: MEK1/2
Vector Backbone Description: Backbone Size:7032; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26942229
Proper citation: RRID:Addgene_72567 Copy
Species: Mus musculus
Genetic Insert: MEK1/2
Vector Backbone Description: Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26942229
Proper citation: RRID:Addgene_72569 Copy
Species: Mus musculus
Genetic Insert: UAS upstream of PSD95-FingR with ZFC(CCR5TC) and KRAB(A)
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR 221; Vector Types:Gateway Cloning; Bacterial Resistance:Ampicillin
Comments: Details of cloning are in:
Son et al., Scientific Reports, 2016. Transgenic FingRs for Live Mapping of Synaptic Dynamics in Genetically-Defined Neurons.
Proper citation: RRID:Addgene_72638 Copy
Species: Mus musculus
Genetic Insert: UAS upstream of GPHN-FingR with ZFI(IL2RFTC) and KRAB(A)
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR 221; Vector Types:Gateway Cloning; Bacterial Resistance:Ampicillin
Comments: PLEASE NOTE- this plasmid has a Stop codon after mCherry which is an error and will cause the construct to not be specifically targeted to the synapse.
The Bonkowsky Lab has a corrected plasmid available which will soon be available at Addgene: pTol2-ziUAS:GPHN.FingR-mKate2-ZFI (IL2RGTC)-KRAB(A).
Details of cloning are in:
Son et al., Scientific Reports, 2016. Transgenic FingRs for Live Mapping of Synaptic Dynamics in Genetically-Defined Neurons.
Proper citation: RRID:Addgene_72639 Copy
Species: Mus musculus
Genetic Insert: Gata 4
Vector Backbone Description: Backbone Size:11391; Vector Backbone:pSAM2_GW_mCherry; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26877223
Proper citation: RRID:Addgene_72690 Copy
Species: Mus musculus
Genetic Insert: PICK1
Vector Backbone Description: Backbone Marker:Oligoengine; Backbone Size:5300; Vector Backbone:pSuper; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21307255
Proper citation: RRID:Addgene_72575 Copy
Species: Mus musculus
Genetic Insert: Islet1
Vector Backbone Description: Backbone Size:11391; Vector Backbone:pSAM2_GW_mCherry; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26877223
Proper citation: RRID:Addgene_72696 Copy
Species: Mus musculus
Genetic Insert: Dub3
Vector Backbone Description: Vector Backbone:pCDN3-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24207026
Proper citation: RRID:Addgene_72683 Copy
Species: Mus musculus
Genetic Insert: CRTC3
Vector Backbone Description: Backbone Marker:Luk Parijs, Addgene Plasmid #11795; Backbone Size:7649; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22232675
Proper citation: RRID:Addgene_72712 Copy
Species: Mus musculus
Genetic Insert: Drp1
Vector Backbone Description: Backbone Marker:EMD Millipore (Novagen); Vector Backbone:pET21b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26446846
Proper citation: RRID:Addgene_72927 Copy
Species: Mus musculus
Genetic Insert: Drp1(deltaIB)
Vector Backbone Description: Backbone Marker:EMD Millipore (Novagen); Vector Backbone:pET21b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26446846
Proper citation: RRID:Addgene_73041 Copy
Species: Mus musculus
Genetic Insert: Foxg1
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26523971
Proper citation: RRID:Addgene_73047 Copy
Species: Mus musculus
Genetic Insert: mFoxg1
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26523971
Proper citation: RRID:Addgene_73048 Copy
Species: Mus musculus
Genetic Insert: Foxg1
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26523971
Proper citation: RRID:Addgene_73057 Copy
Species: Mus musculus
Genetic Insert: control-APEX
Vector Backbone Description: Backbone Size:6833; Vector Backbone:pEF5B.FRT.DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26585297
Comments: APEX sequence derived from glycine max according to: Rhee et al., Science, 2013
Proper citation: RRID:Addgene_73187 Copy
Species: Mus musculus
Genetic Insert: sHRPb-NLG
Vector Backbone Description: Backbone Size:4247; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27240195
Comments: sHRPb is the small split HRP fragment. It consists of amino acids 214-308 of horseradish peroxidase (HRP) with the following 2 mutations: N255D, L299R
The insert contains the following features:
EcoRI-NLG1 ss-AgeI-sHRPb-5 aa linker-V5 epitope tag-MfeI-NLG1-stop-BglII-NotI
CAG promoter
5 aa linker: GSGSG
V5 epitope tag: GKPIPNPLLGLDST
NLG: mouse neuroligin1
This construct was derived from the 3xAP-NLG1 plasmid (Addgene ID 43923).
Proper citation: RRID:Addgene_73144 Copy
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