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Species: Synthetic
Genetic Insert: spNW15
Vector Backbone Description: Vector Backbone:pet28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34521837
Proper citation: RRID:Addgene_173483 Copy
Species: Synthetic
Genetic Insert: spMSP1D1
Vector Backbone Description: Vector Backbone:pet28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34521837
Proper citation: RRID:Addgene_173482 Copy
Species: Other
Genetic Insert: Bovine Rhodopsin promoter driving DsRed fluorescent protein
Vector Backbone Description: Backbone Marker:n/a; Backbone Size:3200; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23818646
Proper citation: RRID:Addgene_173490 Copy
Species: Homo sapiens
Genetic Insert: 4EHP
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFlag-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17289916
Proper citation: RRID:Addgene_17353 Copy
Genetic Insert: IRES-GFP
Vector Backbone Description: Backbone Marker:Nolan Lab; Backbone Size:6332; Vector Backbone:pBMN; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Comments: The pBMN backbone with IRES allowing for expression of GFP. Please note that the sequence information has some inaccuracies, so restriction digests may give slightly different bands than predicted.
This plasmid is extremely slow growing and may need 2 days to grow.
Proper citation: RRID:Addgene_1736 Copy
Species: Caenorhabditis elegans
Genetic Insert: Prps-0::HygR::unc-54 3'UTR; Psqt-1::sqt-1::sqt-1 3' UTR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34849800
Comments: Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173755 Copy
Species: Homo sapiens
Genetic Insert: SENP1
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFlag-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17981124
Comments: See author's map for more information.
I193F and T207A mutations are not known to affect function.
Proper citation: RRID:Addgene_17357 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript SKII (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11784061
Proper citation: RRID:Addgene_173686 Copy
Species: Homo sapiens
Genetic Insert: SUMO1
Vector Backbone Description: Backbone Size:0; Vector Backbone:SRa; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9452416
Comments: See author's map for more information.
Proper citation: RRID:Addgene_17359 Copy
Species: Other
Genetic Insert: Blasticidin S deaminase
Vector Backbone Description: Backbone Size:14873; Vector Backbone:lentiCRISPR v2 (Addgene #52961); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34524848
Proper citation: RRID:Addgene_167186 Copy
Species: Other
Genetic Insert: GFP-GUS
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8000; Vector Backbone:pB7m34GW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28784835
Proper citation: RRID:Addgene_167122 Copy
Species: Synthetic
Genetic Insert: HaloTag7
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET51b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34339177
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.04.13.439540v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167266 Copy
Species: Homo sapiens
Genetic Insert: MEFV
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3800; Vector Backbone:pEntr1A; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35759198
Proper citation: RRID:Addgene_167020 Copy
Species: SARS-CoV-2
Genetic Insert: Nsp1 (residues 13-127)
Vector Backbone Description: Vector Backbone:pMCSG53; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33319167
Proper citation: RRID:Addgene_167256 Copy
Species: Homo sapiens
Genetic Insert: MEFV
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3200; Vector Backbone:pENTR1A; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35759198
Comments: the pENTR1A backbone might impose some restriction
Proper citation: RRID:Addgene_167018 Copy
Vector Backbone Description: Backbone Marker:Depositing Lab: George Church; Backbone Size:3914; Vector Backbone:gRNA_Cloning Vector (Plasmid #41824); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30943414
Proper citation: RRID:Addgene_167165 Copy
Species: Homo sapiens
Genetic Insert: Human LEF-1
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14594802
Comments: Description: This is a wild type LEF-1, cloned into pCS2+.
Reference: Made by Ken-Ichi Takemaru. It is an Eco-R1-blunted HindIII fragment cloned into EcoR1/Stu1 sites.
Proper citation: RRID:Addgene_16709 Copy
Species: Mus musculus
Genetic Insert: dnPKA
Vector Backbone Description: Backbone Size:4100; Vector Backbone:CS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8812120
Comments: Insert: dominant negative Protein Kinase A-bright green flourescent protein fusion protein.
To make sense RNA, for expression, cut with Not1 and transcribe with SP6.
Plasmid History:
Constructed by Anne Ungar in 1996.
Citation: Ungar and Moon (1996)
Developmental Biology 178:186-191.
Proper citation: RRID:Addgene_16716 Copy
Species: Escherichia coli, Neonothopanus nambi
Genetic Insert: caffeic acid cycle genes
Vector Backbone Description: Backbone Size:4839; Vector Backbone:Level2-like; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32341562
Comments: Encodes 5 transcription units for expression in plants: Kanamycin resistance cassette, Neonothopanus nambi hispidin synthase, Neonothopanus nambi hispidin-3-hydroxylase, Neonothopanus nambi luciferase, Neonothopanus nambi caffeoylpiruvate hydrolase
The depositing lab does not recommend this plasmid for most applications, and recommend using pNK3071 instead (https://www.addgene.org/219755/)
Proper citation: RRID:Addgene_167156 Copy
Species: Synthetic
Genetic Insert: SNAPf-tag
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET51b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34339177
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.04.13.439540v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167271 Copy
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