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Species: Rattus norvegicus
Genetic Insert: PACT-mOrange2-hCM1
Vector Backbone Description: Backbone Size:6972; Vector Backbone:pBeta-actin-Nedd1-mOrange2-hCM1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36796357
Proper citation: RRID:Addgene_196868 Copy
Species: Rattus norvegicus
Genetic Insert: Akap9
Vector Backbone Description: Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36796357
Comments: For successful transformation of Plasmid 196867: pCR-XL-rAkap9, it is recommended to use electroporation and to grow transformed bacteria at no more than 28 °C for two days and with 30 μg/ml kanamycin on LB plates for full length plasmid production
Proper citation: RRID:Addgene_196867 Copy
Species: Rattus norvegicus
Genetic Insert: Nedd1-mOrange2-rCM1
Vector Backbone Description: Backbone Size:8089; Vector Backbone:pBeta-actin-Nedd1-mOrange2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36796357
Proper citation: RRID:Addgene_196861 Copy
Species: Rattus norvegicus
Genetic Insert: Nedd1-mOrange2
Vector Backbone Description: Backbone Size:6040; Vector Backbone:pBeta-actin-Tubg1-mEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36796357
Proper citation: RRID:Addgene_196860 Copy
Species: Rattus norvegicus
Genetic Insert: Nedd1-mOrange2-F75A
Vector Backbone Description: Backbone Size:8089; Vector Backbone:pBeta-actin-Nedd1-mOrange2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36796357
Proper citation: RRID:Addgene_196862 Copy
Species: Rattus norvegicus
Genetic Insert: AcGFP-Akap9 (128-425)
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pBeta-actin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36796357
Proper citation: RRID:Addgene_196871 Copy
Species: Rattus norvegicus
Genetic Insert: Synaptotagmin 4
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22398727
Comments: The first codon of the Synpatotagmin has been removed
Proper citation: RRID:Addgene_184505 Copy
Species: Rattus norvegicus
Genetic Insert: td-mCherry-Cdk5rap2
Vector Backbone Description: Backbone Size:7437; Vector Backbone:pBeta-actin-td-mCherry-CI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36796357
Proper citation: RRID:Addgene_196859 Copy
Species: Rattus norvegicus
Genetic Insert: AcGFP-Cdk5rap2-CTD
Vector Backbone Description: Backbone Size:6748; Vector Backbone:pBeta-actin-AcGFP-CI ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36796357
Proper citation: RRID:Addgene_196853 Copy
Species: Rattus norvegicus
Genetic Insert: enNTS1
Vector Backbone Description: Backbone Marker:Internal Lab Plasmid; Backbone Size:6112; Vector Backbone:pDS170; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36680775
Comments: For additional information, please refer to:
Bumbak et al. 2018 "Optimization and 13CH3 methionine labeling of a signaling competent neurotensin receptor 1 variant for NMR studies" BBA Biomembranes. https://doi.org/10.1016/j.bbamem.2018.03.020
Bumbak et al. 2019 "Expression and Purification of a Functional E. coli 13CH3-Methionine-Labeled Thermostable Neurotensin Receptor 1 Variant for Solution NMR Studies" Methods Mol Biol. https://doi.org/10.1007/978-1-4939-9121-1_3
Proper citation: RRID:Addgene_199152 Copy
Species: Rattus norvegicus
Genetic Insert: Map1lc3b
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3900; Vector Backbone:pEGFPc/PGK; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37133994
Proper citation: RRID:Addgene_200083 Copy
Species: Rattus norvegicus
Genetic Insert: Shank3
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: This is a validated antigen plasmid of Anti-Shank3 [N69/46R].
Proper citation: RRID:Addgene_197335 Copy
Species: Rattus norvegicus
Genetic Insert: HA-BioID2-BioID2-Kir4.1
Vector Backbone Description: Backbone Marker:Khakh lab; Backbone Size:5110; Vector Backbone:PZac2.1; Vector Types:Bacterial Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37046092
Proper citation: RRID:Addgene_176746 Copy
Species: Rattus norvegicus
Genetic Insert: AP-3 μ3A
Vector Backbone Description: Backbone Size:8117; Vector Backbone:pACT2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24229647
Proper citation: RRID:Addgene_198174 Copy
Species: Rattus norvegicus
Genetic Insert: AP-3 μ3A
Vector Backbone Description: Backbone Size:5474; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24831812
Comments: μ3A + linker + HA tag subcloned between EcoRI and SalI sites; double HA tag subcloned between SalI and NotI sites (SalI site destroyed)
Proper citation: RRID:Addgene_198179 Copy
Species: Rattus norvegicus
Genetic Insert: Pan-Neurofascin (extracellular)
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9562181
Comments: This is a validated antigen plasmid of Anti-Pan-Neurofascin (extracellular) [A12/18R].
Additional reference: https://pubmed.ncbi.nlm.nih.gov/10931875/.
Proper citation: RRID:Addgene_197290 Copy
Species: Rattus norvegicus
Genetic Insert: ND6-W82-L15 TALEN
Vector Backbone Description: Vector Backbone:pGL3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37058569
Comments: These plasmids may not be suitable for sequencing with NGS, because they contain RVD array. In our lab, we perform Sanger sequencing using the following primers: RVD seq Fwd TGACCGCAGTGGAGGCAGTG; RVD seq Rev TTCACTGCATCCAGCGCAGG
Proper citation: RRID:Addgene_198860 Copy
Species: Rattus norvegicus
Genetic Insert: mCh-V5-NES-EGFP-Erbb2 NLS
Vector Backbone Description: Backbone Marker:Addgene #25752; Vector Backbone:pEN_TTmiRC2; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37055441
Proper citation: RRID:Addgene_192317 Copy
Species: Rattus norvegicus
Genetic Insert: mCh-V5-NES-EGFP-Erbb2 NLS
Vector Backbone Description: Backbone Marker:Addgene #25736; Vector Backbone:pSLIK zeo; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37055441
Proper citation: RRID:Addgene_192339 Copy
Species: Rattus norvegicus
Genetic Insert: Calmodulin 2
Vector Backbone Description: Backbone Size:5335; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37516972
Proper citation: RRID:Addgene_198200 Copy
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