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Species: Eubacterium rectale
Genetic Insert: huErCas12a
Vector Backbone Description: Backbone Marker:Scott Gradia Lab; Backbone Size:6462; Vector Backbone:pET; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: The vector is obtained from Scott Gradia Lab (Addgene #29656) while the gene is obtained from Stephen Ekker Lab (Addgene #132641) . Read the preprint on medRxiv here: https://www.medrxiv.org/content/10.1101/2021.07.21.21260653v1
Proper citation: RRID:Addgene_174685 Copy
Species: Prevotella bryantii B14
Genetic Insert: huPb2Cas12a
Vector Backbone Description: Backbone Marker:Scott Gradia Lab; Backbone Size:6462; Vector Backbone:pET; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: The vector is obtained from Scott Gradia Lab (Addgene #29656) while the gene is obtained from Feng Zhang Lab (Addgene #92272) . Read the preprint on medRxiv here: https://www.medrxiv.org/content/10.1101/2021.07.21.21260653v1
Proper citation: RRID:Addgene_174686 Copy
Species: Thiomicrospira sp. XS5
Genetic Insert: huTsCas12a
Vector Backbone Description: Backbone Marker:Scott Gradia Lab; Backbone Size:6462; Vector Backbone:pET; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: The vector is obtained from Scott Gradia Lab (Addgene #29656) while the gene is obtained from Feng Zhang Lab (Addgene #92267) . Read the preprint on medRxiv here: https://www.medrxiv.org/content/10.1101/2021.07.21.21260653v1
Proper citation: RRID:Addgene_174687 Copy
Species: Francisella novicida
Genetic Insert: FnCas12a
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33608525
Proper citation: RRID:Addgene_174658 Copy
Species: Homo sapiens
Genetic Insert: PARP1-K893I
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-1014 (V762A, K893I) (see PMID: 8473297 for detail about the mutation)
Proper citation: RRID:Addgene_174799 Copy
Species: Homo sapiens
Genetic Insert: PARP1-W318R
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-1014 (W318R, V762A) (see PMID: 26626480 for detail about the mutation)
Proper citation: RRID:Addgene_174793 Copy
Species: Homo sapiens
Genetic Insert: PARP1-A762V
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-1014 (see PMID: 20399804 for detail about the mutation)
Proper citation: RRID:Addgene_174795 Copy
Species: Homo sapiens
Genetic Insert: PARP1-A774L
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-1014 (V762A, A774L) (*protein expression may be toxic to host, add 10 mM benzamide to all growth media, see PMID: 28695525 for protocol) (see PMID: 26626480 for detail about the mutation)
Proper citation: RRID:Addgene_174796 Copy
Species: Homo sapiens
Genetic Insert: PARP1-A870L
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-1014 (V762A, A870L) (*protein expression may be toxic to host, add 10 mM benzamide to all growth media, see PMID: 28695525 for protocol) (see PMID: 26626480 for detail about the mutation)
Proper citation: RRID:Addgene_174797 Copy
Species: X. perforans
Genetic Insert: GG_XopJ4, Kmr plant selection marker
Vector Backbone Description: Backbone Marker:https://www.addgene.org/48076/; Vector Backbone:pICH86988; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34633454
Proper citation: RRID:Addgene_174783 Copy
Species: N. benthamiana
Genetic Insert: GG_synthesized NbJIM2 (NbJIM2syn)-3xFLAG, Kmr plant selection marker
Vector Backbone Description: Backbone Marker:https://www.addgene.org/48076/; Vector Backbone:pICH86988; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34633454
Proper citation: RRID:Addgene_174785 Copy
Species: N. benthamiana
Genetic Insert: GG_NbZAR1syn-L17E/D481V-6xHA, Kmr plant selection marker
Vector Backbone Description: Backbone Marker:https://www.addgene.org/48076/; Vector Backbone:pICH86988; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34633454
Proper citation: RRID:Addgene_174777 Copy
Species: N. benthamiana
Genetic Insert: GG_NbZAR1syn-eGFP, Kmr plant selection marker
Vector Backbone Description: Backbone Marker:https://www.addgene.org/48076/; Vector Backbone:pICH86988; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34633454
Proper citation: RRID:Addgene_174778 Copy
Species: Homo sapiens
Genetic Insert: ALC1-K77R
Vector Backbone Description: Vector Backbone:pET41; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-897 (a start codon included in-frame)
Please note: Plasmid contains R25P and S743C natural variants in ALC1.
Proper citation: RRID:Addgene_174813 Copy
Species: Homo sapiens
Genetic Insert: ALC1-E175Q
Vector Backbone Description: Vector Backbone:pET41; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34465625
Comments: Protein residues 1-897 (a start codon included in-frame).
Please note: Plasmid contains R25P and S743C natural variants in ALC1.
Proper citation: RRID:Addgene_174814 Copy
Genetic Insert: Enhanced green fluorescent protein shRNA #2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2556; Vector Backbone:pENTR1A; Vector Types:RNAi, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19657394
Comments: shRNA sequence is- 5'-CCGCTGGAGTACAACTACAACTTCAAGAGAGTTGTAGTTGTACTCCAGCTTTTT
Proper citation: RRID:Addgene_17471 Copy
Genetic Insert: Firefly Luciferase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2240; Vector Backbone:pENTR4; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17473 Copy
Species: N. benthamiana
Genetic Insert: GG_NbZAR1syn-L17E-4xMyc, Kmr plant selection marker
Vector Backbone Description: Backbone Marker:https://www.addgene.org/48076/; Vector Backbone:pICH86988; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34633454
Proper citation: RRID:Addgene_174772 Copy
Species: N. benthamiana
Genetic Insert: GG_NbZAR1syn-L17E/D481V-4xMyc, Kmr plant selection marker
Vector Backbone Description: Backbone Marker:https://www.addgene.org/48076/; Vector Backbone:pICH86988; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34633454
Proper citation: RRID:Addgene_174773 Copy
Species: N. benthamiana
Genetic Insert: GG_NbZAR1syn-6xHA, Kmr plant selection marker
Vector Backbone Description: Backbone Marker:https://www.addgene.org/48076/; Vector Backbone:pICH86988; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34633454
Proper citation: RRID:Addgene_174774 Copy
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