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Species: Rhodobactor
Genetic Insert: crtEBI operon
Vector Backbone Description: Vector Backbone:FTV; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24952589
Comments: Needs to be grown in E_IF3_15 strain for overproduction of neurosporene
Proper citation: RRID:Addgene_63810 Copy
Species: Synthetic
Genetic Insert: mRFP1
Vector Backbone Description: Vector Backbone:FTV; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24952589
Proper citation: RRID:Addgene_63823 Copy
Species: Synthetic
Genetic Insert: mRFP1
Vector Backbone Description: Vector Backbone:FTV; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24952589
Proper citation: RRID:Addgene_63824 Copy
Species: Synthetic
Genetic Insert: mRFP1
Vector Backbone Description: Vector Backbone:FTV; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24952589
Proper citation: RRID:Addgene_63821 Copy
Species: Synthetic
Genetic Insert: mRFP1
Vector Backbone Description: Vector Backbone:FTV; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24952589
Proper citation: RRID:Addgene_63822 Copy
Species: Synthetic
Genetic Insert: CFP, mRFP1 and GFPmut3b
Vector Backbone Description: Vector Backbone:FTV3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26117546
Proper citation: RRID:Addgene_63828 Copy
Species: Synthetic
Genetic Insert: CFP, mRFP1 and GFPmut3b
Vector Backbone Description: Vector Backbone:FTV3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26117546
Proper citation: RRID:Addgene_63836 Copy
Species: Synthetic
Genetic Insert: CFP, mRFP1 and GFPmut3b
Vector Backbone Description: Vector Backbone:FTV3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26117546
Proper citation: RRID:Addgene_63832 Copy
Species: Synthetic
Genetic Insert: CFP, mRFP1 and GFPmut3b
Vector Backbone Description: Vector Backbone:FTV3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26117546
Proper citation: RRID:Addgene_63833 Copy
Species: Synthetic
Genetic Insert: No-aptamer control mRNA
Vector Backbone Description: Vector Backbone:pFTV1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26621913
Proper citation: RRID:Addgene_63848 Copy
Species: Synthetic
Genetic Insert: DNT3 DNT riboswitch
Vector Backbone Description: Vector Backbone:pFTV1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26621913
Proper citation: RRID:Addgene_63852 Copy
Species: Synthetic
Genetic Insert: Theo45 theophylline riboswitch
Vector Backbone Description: Vector Backbone:pFTV1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26621913
Proper citation: RRID:Addgene_63850 Copy
Species: Homo sapiens
Genetic Insert: TT Fab
Vector Backbone Description: Backbone Size:3500; Vector Backbone:pAra; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Provided with the pComb3H system for arabinose induced expression of soluble protein with detection tags. The “TT” refers to the human Fab to tetanus toxin which can be used as a protein expression control. Alternatively, one could upgrade to the pComb3X system, also available on Addgene, which already contains tags.
Select reference:
Cary, S.P.; Lee, J.; Wagenknecht, R.; Silverman, G.J. (2000) Characterization of Superantigen-Induced Clonal Deletion with a Novel Clan III-Restricted Avian Monoclonal Antibody: Exploiting Evolutionary Distance to Create Antibodies Specific for a Conserved VH Region Surface. J. Immun., 164, 4730-4741.
Proper citation: RRID:Addgene_63901 Copy
Vector Backbone Description: Backbone Marker:DNA 2.0; Backbone Size:3792; Vector Backbone:pJ344; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26133196
Proper citation: RRID:Addgene_64026 Copy
Species: Arabidopsis thaliana
Genetic Insert: zep
Vector Backbone Description: Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:10040; Vector Backbone:pAC-ZEAXipi; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24506237
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53087 Copy
Species: Rhodobacter capsulatus
Genetic Insert: crtI
Vector Backbone Description: Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:8106; Vector Backbone:pAC-PHYT; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:7919981
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53271 Copy
Species: Arabidopsis thaliana
Genetic Insert: lcyE
Vector Backbone Description: Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:9476; Vector Backbone:pAC-LYC; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:8798688
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53273 Copy
Species: Synechococcus PCC7942
Genetic Insert: pds
Vector Backbone Description: Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:8106; Vector Backbone:pAC-PHYT; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:7919981
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53316 Copy
Species: Erwinia herbicola (Pantoea agglomerans) Eho10
Genetic Insert: crtE, crtY, crtI
Vector Backbone Description: Backbone Marker:New England BioLabs; Backbone Size:4245; Vector Backbone:pACYC184; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17634749
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53282 Copy
Species: Erwinia herbicola Eho10
Genetic Insert: crtE, idi, crtY, crtI, crtB
Vector Backbone Description: Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:13581; Vector Backbone:pAC-EHER; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17634749
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53283 Copy
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