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  • Bacterial Resistance:kanamycin (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
6xHis-MBP-huErCas12a
 
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RRID:Addgene_174685 huErCas12a Eubacterium rectale Kanamycin The vector is obtained from Scott Gradia Lab (Addgene #29656) while the gene is obtained from Stephen Ekker Lab (Addgene #132641) . Read the preprint on medRxiv here: https://www.medrxiv.org/content/10.1101/2021.07.21.21260653v1 Backbone Marker:Scott Gradia Lab; Backbone Size:6462; Vector Backbone:pET; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin Q926R mutation is observed as indicated from the lab the gene was obtained, but the mutation does not affect ErCas12a function 2026-08-15 01:10:43 0
6xHis-MBP-huPb2Cas12a
 
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RRID:Addgene_174686 huPb2Cas12a Prevotella bryantii B14 Kanamycin The vector is obtained from Scott Gradia Lab (Addgene #29656) while the gene is obtained from Feng Zhang Lab (Addgene #92272) . Read the preprint on medRxiv here: https://www.medrxiv.org/content/10.1101/2021.07.21.21260653v1 Backbone Marker:Scott Gradia Lab; Backbone Size:6462; Vector Backbone:pET; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:10:43 0
6xHis-MBP-huTsCas12a
 
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RRID:Addgene_174687 huTsCas12a Thiomicrospira sp. XS5 Kanamycin The vector is obtained from Scott Gradia Lab (Addgene #29656) while the gene is obtained from Feng Zhang Lab (Addgene #92267) . Read the preprint on medRxiv here: https://www.medrxiv.org/content/10.1101/2021.07.21.21260653v1 Backbone Marker:Scott Gradia Lab; Backbone Size:6462; Vector Backbone:pET; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:10:43 0
pET28-FnCas12a-TEV
 
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RRID:Addgene_174658 FnCas12a Francisella novicida Kanamycin PMID:33608525 Vector Backbone:pET28; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin 2026-08-15 01:10:42 0
pET28_6xHis-PARP1-K893I
 
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RRID:Addgene_174799 PARP1-K893I Homo sapiens Kanamycin PMID:34465625 Protein residues 1-1014 (V762A, K893I) (see PMID: 8473297 for detail about the mutation) Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin K893I, V762A 2026-08-15 01:10:44 0
pET28_6xHis-PARP1-W318R
 
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RRID:Addgene_174793 PARP1-W318R Homo sapiens Kanamycin PMID:34465625 Protein residues 1-1014 (W318R, V762A) (see PMID: 26626480 for detail about the mutation) Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin W318, V762A 2026-08-15 01:10:44 0
pET28_6xHis-PARP1-A762V
 
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RRID:Addgene_174795 PARP1-A762V Homo sapiens Kanamycin PMID:34465625 Protein residues 1-1014 (see PMID: 20399804 for detail about the mutation) Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin A762V 2026-08-15 01:10:44 0
pET28_6xHis-PARP1-A774L
 
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RRID:Addgene_174796 PARP1-A774L Homo sapiens Kanamycin PMID:34465625 Protein residues 1-1014 (V762A, A774L) (*protein expression may be toxic to host, add 10 mM benzamide to all growth media, see PMID: 28695525 for protocol) (see PMID: 26626480 for detail about the mutation) Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin A774L, V762A 2026-08-15 01:10:44 0
pET28_6xHis-PARP1-A870L
 
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RRID:Addgene_174797 PARP1-A870L Homo sapiens Kanamycin PMID:34465625 Protein residues 1-1014 (V762A, A870L) (*protein expression may be toxic to host, add 10 mM benzamide to all growth media, see PMID: 28695525 for protocol) (see PMID: 26626480 for detail about the mutation) Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin A870L, V762A 2026-08-15 01:10:44 0
pZA72
 
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RRID:Addgene_174783 GG_XopJ4, Kmr plant selection marker X. perforans Kanamycin PMID:34633454 Backbone Marker:https://www.addgene.org/48076/; Vector Backbone:pICH86988; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin WT + STOP codon 2026-08-15 01:10:44 0
pZA71
 
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RRID:Addgene_174785 GG_synthesized NbJIM2 (NbJIM2syn)-3xFLAG, Kmr plant selection marker N. benthamiana Kanamycin PMID:34633454 Backbone Marker:https://www.addgene.org/48076/; Vector Backbone:pICH86988; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin WT 2026-08-15 01:10:44 0
pZA64
 
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RRID:Addgene_174777 GG_NbZAR1syn-L17E/D481V-6xHA, Kmr plant selection marker N. benthamiana Kanamycin PMID:34633454 Backbone Marker:https://www.addgene.org/48076/; Vector Backbone:pICH86988; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin L17E/D481V 2026-08-15 01:10:44 0
pZA65
 
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RRID:Addgene_174778 GG_NbZAR1syn-eGFP, Kmr plant selection marker N. benthamiana Kanamycin PMID:34633454 Backbone Marker:https://www.addgene.org/48076/; Vector Backbone:pICH86988; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin WT 2026-08-15 01:10:44 0
pET41sIII_GST-ALC1-K77R
 
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RRID:Addgene_174813 ALC1-K77R Homo sapiens Kanamycin PMID:34465625 Protein residues 1-897 (a start codon included in-frame) Please note: Plasmid contains R25P and S743C natural variants in ALC1. Vector Backbone:pET41; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin K77R 2026-08-15 01:10:44 0
pET41sIII_GST-ALC1-E175Q
 
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RRID:Addgene_174814 ALC1-E175Q Homo sapiens Kanamycin PMID:34465625 Protein residues 1-897 (a start codon included in-frame). Please note: Plasmid contains R25P and S743C natural variants in ALC1. Vector Backbone:pET41; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin E175Q 2026-08-15 01:10:44 0
pENTR/pTER shEGFP #2 (728-2)
 
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RRID:Addgene_17471 Enhanced green fluorescent protein shRNA #2 Kanamycin PMID:19657394 shRNA sequence is- 5'-CCGCTGGAGTACAACTACAACTTCAAGAGAGTTGTAGTTGTACTCCAGCTTTTT Backbone Marker:Invitrogen; Backbone Size:2556; Vector Backbone:pENTR1A; Vector Types:RNAi, Gateway Cloning; Bacterial Resistance:Kanamycin 2026-08-15 01:10:43 0
pENTR-LUC (w158-1)
 
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1+ mentions
RRID:Addgene_17473 Firefly Luciferase Kanamycin PMID:19657394 Backbone Marker:Invitrogen; Backbone Size:2240; Vector Backbone:pENTR4; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin 2026-08-15 01:10:43 5
pZA59
 
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RRID:Addgene_174772 GG_NbZAR1syn-L17E-4xMyc, Kmr plant selection marker N. benthamiana Kanamycin PMID:34633454 Backbone Marker:https://www.addgene.org/48076/; Vector Backbone:pICH86988; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin L17E 2026-08-15 01:10:43 0
pZA60
 
Resource Report
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RRID:Addgene_174773 GG_NbZAR1syn-L17E/D481V-4xMyc, Kmr plant selection marker N. benthamiana Kanamycin PMID:34633454 Backbone Marker:https://www.addgene.org/48076/; Vector Backbone:pICH86988; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin L17E/D481V 2026-08-15 01:10:43 0
pZA61
 
Resource Report
Resource Website
RRID:Addgene_174774 GG_NbZAR1syn-6xHA, Kmr plant selection marker N. benthamiana Kanamycin PMID:34633454 Backbone Marker:https://www.addgene.org/48076/; Vector Backbone:pICH86988; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin WT 2026-08-15 01:10:43 0

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