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Species: Erwinia herbicola Eho10
Genetic Insert: crtY, crtI, crtB
Vector Backbone Description: Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:10609; Vector Backbone:pAC-BETA; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17634749
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53281 Copy
Species: Synechococcus PCC7942
Genetic Insert: pds
Vector Backbone Description: Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:13581; Vector Backbone:pAC-EHER; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17634749
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53284 Copy
Species: Haematococcus pluvialis
Genetic Insert: crtO
Vector Backbone Description: Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:9259; Vector Backbone:pAC-BETAipi; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17634749
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53301 Copy
Species: Erwinia herbicola Eho10
Genetic Insert: crtE, crtB
Vector Backbone Description: Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:13581; Vector Backbone:pAC-EHER; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:7919981
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53300 Copy
Species: Erwinia herbicola Eho10
Genetic Insert: crtE, idi, crtX, crtY, crtI, crtB, crtZ
Vector Backbone Description: Backbone Marker:New England BioLabs; Backbone Size:4245; Vector Backbone:pACYC184; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:7919981
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53262 Copy
Species: E. coli
Genetic Insert: etpC
Vector Backbone Description: Backbone Size:3719; Vector Backbone:pACYC184; Vector Types:Bacterial Expression, pACYC184; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:19707189
Comments: Please note that there is a L to M mutation in etpC. This mutation should not affect function.
Proper citation: RRID:Addgene_53533 Copy
Species: e. coli
Genetic Insert: pLux
Vector Backbone Description: Backbone Size:1000; Vector Backbone:pLuxR; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24104480
Proper citation: RRID:Addgene_53544 Copy
Genetic Insert: None
Vector Backbone Description: Vector Backbone:pCAndy; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24550272
Proper citation: RRID:Addgene_53571 Copy
Species: Other
Genetic Insert: λCI-β-flap fusion
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:3142; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:9121589
Comments: Note-When cloning into this plasmid, the 5' end of the insert must provide an extra nucleotide to maintain the reading frame. Additionally, a stop codon must be inserted 5' to the BamHI site. Please see supplemental document for more details.
This plasmid is used in conjuction with the other Ann Hochschild Bacterial 2-hybrid system plasmids (Addgene plasmids 53730, 53731, 53732, 53733, and 53734) and must be used in the reporter strain, FW102 OL2–62 (Addgene item 53735). Please see the associated article for links to all of these plasmids:
http://www.addgene.org/browse/article/8393/
The following articles can provide more information on using the bacterial 2-hybrid system:
Dove, et al. 1997 PMID 9121589
Dove and Hochschild 1998 PMID 9499408
Deaconescu, et al. 2006 PMID 16469698
Deighan, et al. 2008 PMID 18832144
Kuznedelov, et al. 2002 PMID 11823642
Proper citation: RRID:Addgene_53733 Copy
Species: Unspecified
Genetic Insert: AID*-3HA-mNeonGreen
Vector Backbone Description: Vector Backbone:pBluescript II SK+; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_253584 Copy
Species: Synthetic
Genetic Insert: 5x 2EBS-S10 (A1-A2)
Vector Backbone Description: Vector Backbone:pUPD2; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38379432
Proper citation: RRID:Addgene_254056 Copy
Species: Lambda phage (Addgene #87359)
Genetic Insert: lambdaRED
Vector Backbone Description: Backbone Size:3008; Vector Backbone:CloDF13; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:42067623
Proper citation: RRID:Addgene_253997 Copy
Species: Synthetic
Genetic Insert: cat
Vector Backbone Description: Vector Backbone:pR6K; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40927394
Proper citation: RRID:Addgene_243900 Copy
Species: Synthetic
Genetic Insert: tetA
Vector Backbone Description: Vector Backbone:pR6K; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40927394
Proper citation: RRID:Addgene_243901 Copy
Species: Other
Genetic Insert: J23119 CAO1 target + GAPDH T17 5x array non-targeting
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253628 Copy
Species: Other
Genetic Insert: J23119 GAPDH T17 target + GAPDH T17 5x array targeting
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253629 Copy
Species: Other
Genetic Insert: J23119 GAPDH T17 target + CAO1 5x array non-targeting
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253630 Copy
Species: Other
Genetic Insert: PxylA
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1A2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_235411 Copy
Species: Other
Genetic Insert: sfGFP Dropout Cassette
Vector Backbone Description: Vector Backbone: pNW33N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_235362 Copy
Species: Other
Genetic Insert: Bba_B0015
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1A2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_235369 Copy
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