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Species: Mus musculus
Genetic Insert: sHRPb-NLG
Vector Backbone Description: Backbone Size:8451; Vector Backbone:FSW lentiviral vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27240195
Comments: sHRPb is the small split HRP fragment. It consists of amino acids 214-308 of horseradish peroxidase (HRP) with the following 2 mutations: N255D, L299R.
The plasmid contains the following features (including promoter region):
PacI-SynapsinP-XbaI-NLG1 ss-AgeI-sHRPb-5 aa linker-V5 epitope tag-MfeI-NLG1-stop-AscI
Human synapsin promoter
5 aa linker: GSGSG
V5 epitope tag: GKPIPNPLLGLDST
Lentiviral vector. Derived from FCGW with human synapsin1 promoter replacing CMV
Proper citation: RRID:Addgene_73148 Copy
Species: Mus musculus
Genetic Insert: spCas9
Vector Backbone Description: Vector Backbone:pX330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25642960
Comments: We cloned 2 guide RNAs and a puromycin cassette.
Proper citation: RRID:Addgene_73131 Copy
Species: Mus musculus
Genetic Insert: Rap1
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pLPC; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20339076
Comments: Additional reference: Cell Rep. 2013 Jun 27;3(6):1847-56.
Proper citation: RRID:Addgene_73135 Copy
Species: Mus musculus
Genetic Insert: BKalpha
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5401; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26301573
Proper citation: RRID:Addgene_73213 Copy
Species: Mus musculus
Genetic Insert: HDAC7
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5840; Vector Backbone:pEF6/V5-His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23853092
Proper citation: RRID:Addgene_73267 Copy
Species: Mus musculus
Genetic Insert: HDAC7
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5783; Vector Backbone:pEF6/V5-His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23853092
Proper citation: RRID:Addgene_73268 Copy
Species: Mus musculus
Genetic Insert: HDAC7
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5840; Vector Backbone:pEF6/V5-His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23853092
Proper citation: RRID:Addgene_73269 Copy
Species: Mus musculus
Genetic Insert: Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)
Vector Backbone Description: Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19875458
Comments: E16K mutation originated in Plasmid #8706 but does not affect function.
WT control for Plasmid 73364: pCDNA3-STAT3-S727D is Plasmid 8706: Stat3 pcDNA3 from the Darnell lab.
Proper citation: RRID:Addgene_73364 Copy
Species: Mus musculus
Genetic Insert: PKM
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:5000; Vector Backbone:pIRES-hr-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23559014
Comments: PKM (Genebank accession NM_001039079) was amplified with primers 5′-tgcgaattcaatggattctgtcatgccttcc-3′ and 5′-attgtcgactcacacggactcctcagcag-3′ from adult mouse brain cDNA
Proper citation: RRID:Addgene_73405 Copy
Species: Mus musculus
Genetic Insert: ninein coiled-coil 1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15784680
Proper citation: RRID:Addgene_73521 Copy
Species: Mus musculus
Genetic Insert: DGCR8
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 42230); Backbone Size:8506; Vector Backbone:X330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28100686
Proper citation: RRID:Addgene_73528 Copy
Species: Mus musculus
Genetic Insert: DGCR8
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 42230); Backbone Size:8506; Vector Backbone:X330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28100686
Proper citation: RRID:Addgene_73525 Copy
Species: Mus musculus
Genetic Insert: AGO2
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 48138); Backbone Size:9300; Vector Backbone:pSpCas9(BB)-2A-GFP (PX458); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29396181
Proper citation: RRID:Addgene_73531 Copy
Species: Mus musculus
Genetic Insert: AGO2
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 42230); Backbone Size:8506; Vector Backbone:X330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29396181
Proper citation: RRID:Addgene_73530 Copy
Species: Mus musculus
Genetic Insert: AGO1
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 48138); Backbone Size:9300; Vector Backbone:pSpCas9(BB)-2A-GFP (PX458); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29396181
Proper citation: RRID:Addgene_73534 Copy
Species: Mus musculus
Genetic Insert: AGO1
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 48138); Backbone Size:9300; Vector Backbone:pSpCas9(BB)-2A-GFP (PX458); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29396181
Proper citation: RRID:Addgene_73533 Copy
Species: Mus musculus
Genetic Insert: Ago2
Vector Backbone Description: Backbone Marker:David Root (Addgene plasmid # 41393); Backbone Size:9354; Vector Backbone:pCW57.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29396181
Comments: Vector linearized using NheI/BamH1 sites. Insert cloned by recombination using In-Fusion cloning. Note that this plasmid grows slowly. Liquid cultures may need to be incubated up to two days for substantial growth.
Proper citation: RRID:Addgene_73538 Copy
Species: Mus musculus
Genetic Insert: Gata6
Vector Backbone Description: Backbone Marker:David Root (Addgene plasmid # 41393); Backbone Size:9354; Vector Backbone:pCW57.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29396181
Comments: Vector linearized using NheI/BamH1 sites. Insert cloned by recombination using In-Fusion cloning. Note that this plasmid grows slowly. Liquid cultures may need to be incubated up to two days for substantial growth.
Proper citation: RRID:Addgene_73537 Copy
Species: Mus musculus
Genetic Insert: AGO1
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 48138); Backbone Size:9300; Vector Backbone:pSpCas9(BB)-2A-GFP (PX458); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29396181
Proper citation: RRID:Addgene_73536 Copy
Species: Mus musculus
Genetic Insert: DC-STAMP
Vector Backbone Description: Backbone Marker:Sigma Aldrich; Backbone Size:6400; Vector Backbone:3xFlag-Myc-CMV26; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_73583 Copy
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