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Genetic Insert: Luciferase
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4283; Vector Backbone:pGL4.23; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: The XhoI/NcoI fragment containing QUAS-Pmin was subcloned from pQUAS-GG
into pGL4.23 (Promega) to replace its minimal promoter. pGL4.23 contains synthetic firefly
luciferase gene (luc2), which has been codon optimized for high expression in mammalian cells.
Proper citation: RRID:Addgene_24337 Copy
Genetic Insert: Cre
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Worm Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19706417
Comments: ins-Cre_cryaa-Venus was generated by placing the Cre coding sequence downstream of 1.5 kb of the proximal insulin promoter. The cryaa-Venus cassette was inserted downstream of Cre in the reverse orientation using 0.54 kb of the cryaa promoter.
Proper citation: RRID:Addgene_24333 Copy
Species: Danio rerio
Genetic Insert: H2B
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Worm Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19706417
Comments: This plasmid was constructed using 1.5 kb of the hsp70l promoter. The floxed mCherry-STOP and H2b_GFP fragments were PCR amplified individually and sequentially cloned downstream of the hsp70l promoter. The mCherry-STOP cassette serves as a heat-shock induction marker prevents read-through translation of the H2B-GFP fusion protein, which is only expressed in cells that have undergone a Cre mediated excision event. cryaa-Cerulean was inserted downstream of HSB-GFP in the reverse orientation.
*H2B has D26G and V119I mutations. According to the depositor, GFP expression from this construct was always nuclear so there is no reason to suspect that the mutations affect the function (nuclear localization) of the H2B fragment.
Proper citation: RRID:Addgene_24334 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Streptomycin
Defining Citation: PMID:20350301
Comments: F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZ M15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara, leu) 7649 galU galK rspL nupG [ λcI857 (cro-bioA) < > araC-PBADtrfA]
Proper citation: RRID:Addgene_24545 Copy
Species: Homo sapiens
Genetic Insert: transcription factor Sp3
Vector Backbone Description: Backbone Marker:Guntram Suske, Addgene plasmid 24544; Backbone Size:4000; Vector Backbone:pN3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15247228
Comments: Two nucleotide mismatches with author sequence detected (one adjacent to the EcoRI cloning site and the second in the middle of the 5´-UTR). They do not affect the coding sequence of Sp3.
Proper citation: RRID:Addgene_24541 Copy
Genetic Insert: pN3-Control
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pN3-Control; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15247228
Comments: vector control for pN3-Sp1FL and pN3-Sp3FL vectors
Proper citation: RRID:Addgene_24544 Copy
Species: Homo sapiens
Genetic Insert: Sp1FL
Vector Backbone Description: Backbone Marker:Guntram Suske, Addgene plasmid 24544; Backbone Size:4000; Vector Backbone:pN3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: pN3-Control vector also available from Addgene
2 nucleotide mismatches with author sequence detected in the most 3´-UTR. They do not affect the coding sequence of Sp1.
Proper citation: RRID:Addgene_24543 Copy
Species: Homo sapiens
Genetic Insert: Kinesin
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11086009
Proper citation: RRID:Addgene_24439 Copy
Species: A. victoria
Genetic Insert: eGFP
Vector Backbone Description: Backbone Size:4557; Vector Backbone:pBluescript; Vector Types:Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19966808
Proper citation: RRID:Addgene_24557 Copy
Vector Backbone Description: Backbone Size:4650; Vector Backbone:pBluescript; Vector Types:Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19966808
Comments: TAP tag is 6X his-TEV cleavage site-3X FLAG tag
Proper citation: RRID:Addgene_24556 Copy
Species: Mus musculus
Genetic Insert: Ofd1
Vector Backbone Description: Backbone Size:4501; Vector Backbone:pBluescript; Vector Types:Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19966808
Comments: Also see the following article:
Ofd1, a human disease gene, regulates the length and distal structure of centrioles.
Singla V, Romaguera-Ros M, Garcia-Verdugo JM, Reiter JF.
Dev Cell. 2010 Mar 16;18(3):410-24.PMID: 20230748
Proper citation: RRID:Addgene_24559 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3893; Vector Backbone:pBluescript; Vector Types:Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19966808
Proper citation: RRID:Addgene_24553 Copy
Species: Homo sapiens
Genetic Insert: Kinesin
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11086009
Proper citation: RRID:Addgene_24432 Copy
Genetic Insert: Sleeping Beauty delta DDE
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3712; Vector Backbone:pCMVbeta; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9390559
Proper citation: RRID:Addgene_24552 Copy
Species: Homo sapiens
Genetic Insert: Kinesin
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11086009
Proper citation: RRID:Addgene_24433 Copy
Species: Homo sapiens
Genetic Insert: Kinesin
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11086009
Proper citation: RRID:Addgene_24434 Copy
Vector Backbone Description: Backbone Size:4512; Vector Backbone:pBluescript; Vector Types:Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19966808
Proper citation: RRID:Addgene_24554 Copy
Genetic Insert: Sleeping Beauty
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3712; Vector Backbone:pCMVbeta; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9390559
Proper citation: RRID:Addgene_24551 Copy
Species: Homo sapiens
Genetic Insert: Kinesin
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11086009
Proper citation: RRID:Addgene_24430 Copy
Species: Homo sapiens
Genetic Insert: CLASP2 (340-1362) 9xS/A
Vector Backbone Description: Backbone Marker:Stratagene, ATCC; Backbone Size:33414; Vector Backbone:pAdEasy-1; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19289791
Comments: See the attached file for the old NIH reference sequence. Numbering starts at amino acid 78 and is off by 8 amino acids because of an insertion that is not accounted for.
Due to difficulty in sequencing this plasmid, the EGFP and C-terminus of CLASP2 has not been sequence verified.
EGFP-CLASP2 340-1362 9xS/A was cloned into pShuttle-CMV between KpnI and NotI then recombined by homologous recombination into pAdEasy-1.
AdEasy is a registered trademark of the Johns Hopkins University.
Proper citation: RRID:Addgene_24523 Copy
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