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Species: Homo sapiens
Genetic Insert: p18
Vector Backbone Description: Backbone Marker:BD PharMingen; Backbone Size:4661; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22980980
Proper citation: RRID:Addgene_42331 Copy
Genetic Insert: humanized S. pyogenes Cas9 (D10A) nickase
Vector Backbone Description: Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23287718
Comments: Note, Addgene's quality control sequencing has found a few discrepancies with the depositor's full sequence. The depositing lab is aware of these discrepancies, and they are not thought to affect plasmid function.
For more information on Zhang Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/zhang/
Proper citation: RRID:Addgene_42333 Copy
Genetic Insert: humanized S. pyogenes Cas9 (D10A) nickase
Vector Backbone Description: Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23287718
Comments: Note, Addgene's quality control sequencing has found a few discrepancies with the depositor's full sequence. The depositing lab is aware of these discrepancies, and they are not thought to affect plasmid function.
For more information on Zhang Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/zhang/
Proper citation: RRID:Addgene_42335 Copy
Species: Homo sapiens
Genetic Insert: p18 G2A
Vector Backbone Description: Backbone Marker:BD PharMingen; Backbone Size:4661; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22980980
Proper citation: RRID:Addgene_42327 Copy
Species: Homo sapiens
Genetic Insert: mp1
Vector Backbone Description: Backbone Marker:BD PharMingen; Backbone Size:4661; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22980980
Proper citation: RRID:Addgene_42329 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Prof. Amir Sharon (Tel Aviv University, Israel); Vector Backbone:pSH1; Vector Types:Filamentous Ascomycetes EGFP expression; Bacterial Resistance:Ampicillin
Comments: Plasmid provided by Prof. Amir Sharon (Tel Aviv University, Israel)
The full plasmid sequence is assembled and not the result of sequencing, there are some discrepancies between the Addgene QC sequence and the full plasmid sequence. These differences are in the plasmid backbone regions.
Proper citation: RRID:Addgene_42323 Copy
Species: Homo sapiens
Genetic Insert: RagB D163N
Vector Backbone Description: Backbone Marker:BD PharMingen; Backbone Size:4661; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22980980
Proper citation: RRID:Addgene_42324 Copy
Species: Nostoc punctiforme
Genetic Insert: NpuDnaEC6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4786; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19344715
Proper citation: RRID:Addgene_42305 Copy
Vector Backbone Description: Backbone Marker:Invitrogen (Life Technologies); Backbone Size:3600; Vector Backbone:pMT/BiP/V5-His A; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_42300 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ATG18
Vector Backbone Description: Backbone Size:6112; Vector Backbone:Ycplac111; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22704557
Comments: ATG18 was cloned with 300bp upstream and downstream of the gene. The myc tag was added later by mutagenesis.
Proper citation: RRID:Addgene_42530 Copy
Species: cyanobacteria
Genetic Insert: KaiC CII cat-/EE (E318Q; S431E; T432E)
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:2200; Vector Backbone:prset-b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23277568
Proper citation: RRID:Addgene_42498 Copy
Species: Arabidopsis thaliana
Genetic Insert: FKF1 (N-LOV, G128D mutant) fused with VP16
Vector Backbone Description: Backbone Marker:Invitrogene; Backbone Size:5300; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19801976
Proper citation: RRID:Addgene_42499 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsv2
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHIS2 parallel; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22704557
Proper citation: RRID:Addgene_42524 Copy
Species: Mus musculus
Genetic Insert: Pyruvate Kinase M2 Cys358->Ser
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6800; Vector Backbone:pLHCX; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22052977
Comments: Changed Cys358 codon to Ser358 by 2-step PCR mutagenesis using pLHCX-FT-PKM2 as template
Proper citation: RRID:Addgene_42513 Copy
Species: Mus musculus
Genetic Insert: Pyruvate Kinase M2 Lys433->Glu
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6800; Vector Backbone:pLHCX; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22052977
Comments: Christofk, H. R., Vander Heiden, M. G., Wu, N., Asara, J. M., & Cantley, L. C. (2008). Pyruvate kinase M2 is a phosphotyrosine-binding protein. Nature, 452(7184), 181–186. doi:10.1038/nature06667
Proper citation: RRID:Addgene_42514 Copy
Species: Homo sapiens
Genetic Insert: shRNA-PKM2
Vector Backbone Description: Backbone Marker:The RNAi consortium; Backbone Size:7032; Vector Backbone:pLKO.1; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22052977
Comments: The depositing scientist notes: NM_011099.3 is PKM2 and NM_001253883.1 is PKM1, based on sequence homology of the respective translated products to the human PKM isoforms. The isoform annotations in the corresponding GeneBank entries are incorrect.
Proper citation: RRID:Addgene_42516 Copy
Species: Streptomyces coelicolor A3(2)
Genetic Insert: guanosine ADP-ribosyltransferase
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6703; Vector Backbone:pMAL-p2x; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23212047
Proper citation: RRID:Addgene_42509 Copy
Species: Homo sapiens
Genetic Insert: soluble PACE
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5077; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23205847
Proper citation: RRID:Addgene_42503 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Kex2 protease
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5140; Vector Backbone:pcDNA6/V5-His Version C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23205847
Proper citation: RRID:Addgene_42504 Copy
Species: Streptomyces coelicolor A3(2)
Genetic Insert: guanosine ADP-ribosyltransferase
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6715; Vector Backbone:pMAL-p2x; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23212047
Proper citation: RRID:Addgene_42507 Copy
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