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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pSPB867 Resource Report Resource Website |
RRID:Addgene_128305 | Para - cfp-parB | Ampicillin | PMID:31406375 | Cloning method: Golden Gate. | Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:36 | 0 | ||
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pSPB866 Resource Report Resource Website |
RRID:Addgene_128304 | Para - GCN4zip.MSE-cfp-parB | Ampicillin | PMID:31406375 | Cloning method: Golden Gate. | Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:36 | 0 | ||
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DCT.VV.Orf156 Resource Report Resource Website |
RRID:Addgene_12844 | VV.Orf156 | Vaccinia Virus | Ampicillin | PMID:15623576 | Please see the Vaccinia kit page ( https://www.addgene.org/vvorf/ ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid. | Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA 3.1D V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:37 | 0 | |
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DCT.VV.Orf152 Resource Report Resource Website |
RRID:Addgene_12840 | VV.Orf152 | Vaccinia Virus | Ampicillin | PMID:15623576 | Please see the Vaccinia kit page ( https://www.addgene.org/vvorf/ ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid. | Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA 3.1D V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:37 | 0 | |
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pPEE36 Resource Report Resource Website |
RRID:Addgene_128376 | Neomycin (NEO) | Synthetic | Ampicillin | PMID:31220607 | Contains the Safe Haven Site 7 sequence (CNAG_05557 - CNAG_05558) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site. | Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:36 | 0 | |
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pPEE35 Resource Report Resource Website |
RRID:Addgene_128375 | Neomycin (NEO) | Synthetic | Ampicillin | PMID:31220607 | Contains the Safe Haven Site 6 sequence (CNAG_04586 - CNAG_04585) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site. | Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:36 | 0 | |
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pPEE34 Resource Report Resource Website |
RRID:Addgene_128374 | Neomycin (NEO) | Synthetic | Ampicillin | PMID:31220607 | Contains the Safe Haven Site 5 sequence (CNAG_05670 - CNAG_05671) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site. | Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:36 | 0 | |
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pPEE39 Resource Report Resource Website |
RRID:Addgene_128379 | Hygromycin (HYG) | Synthetic | Ampicillin | PMID:31220607 | Contains the Safe Haven Site 5 sequence (CNAG_05670 - CNAG_05671) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site. | Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:36 | 0 | |
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pNM262 Resource Report Resource Website |
RRID:Addgene_128412 | Lysostaphin | Staphylococcus simulans | Ampicillin | PMID:31792444 | Backbone Size:7499; Vector Backbone:pLG320; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:37 | 0 | ||
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pPEE38 Resource Report Resource Website |
RRID:Addgene_128378 | Hygromycin (HYG) | Synthetic | Ampicillin | PMID:31220607 | Contains the Safe Haven Site 4 sequence (CNAG_06526 - CNAG_06527) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site. | Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:36 | 0 | |
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pPEE37 Resource Report Resource Website |
RRID:Addgene_128377 | Hygromycin (HYG) | Synthetic | Ampicillin | PMID:31220607 | Contains the Safe Haven Site 3 sequence (CNAG_02589 - CNAG_02588) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site. | Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:36 | 0 | |
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pLG371 Resource Report Resource Website |
RRID:Addgene_128410 | gfpmut2 | Synthetic | Ampicillin | PMID:31792444 | Vector Backbone:pLG166; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:37 | 0 | ||
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pCZ47 Resource Report Resource Website 1+ mentions |
RRID:Addgene_128414 | vab-1 | Caenorhabditis elegans | Ampicillin | PMID:9506518 | Backbone Size:3400; Vector Backbone:pSL1190; Vector Types:Unspecified; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:37 | 2 | ||
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DCT.VV.Orf160 Resource Report Resource Website |
RRID:Addgene_12848 | VV.Orf160 | Vaccinia Virus | Ampicillin | PMID:15623576 | Please see the Vaccinia kit page ( https://www.addgene.org/vvorf/ ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid. | Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA 3.1D V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:37 | 0 | |
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DCT.VV.Orf157 Resource Report Resource Website |
RRID:Addgene_12845 | VV.Orf157 | Vaccinia Virus | Ampicillin | PMID:15623576 | Please see the Vaccinia kit page ( https://www.addgene.org/vvorf/ ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid. | Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA 3.1D V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:37 | 0 | |
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pET28a-C9ORF80 Resource Report Resource Website 1+ mentions |
RRID:Addgene_128418 | C9ORF80 | Homo sapiens | Kanamycin | PMID:29150435 | Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Addgene | 2026-10-24 02:05:37 | 2 | ||
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pPEE32 Resource Report Resource Website |
RRID:Addgene_128372 | Neomycin (NEO) | Synthetic | Ampicillin | PMID:31220607 | Contains the Safe Haven Site 3 sequence (CNAG_02589 - CNAG_02588) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site. | Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:36 | 0 | |
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pPEE21 Resource Report Resource Website |
RRID:Addgene_128364 | Acetamidase (amdS) | Synthetic | Ampicillin | PMID:31220607 | Contains the Safe Haven Site 5 sequence (CNAG_05670 - CNAG_05671) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site. | Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:36 | 0 | |
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pPEE28 Resource Report Resource Website |
RRID:Addgene_128368 | Noureothricin (NAT) | Synthetic | Ampicillin | PMID:31220607 | Contains the Safe Haven Site 4 sequence (CNAG_06526 - CNAG_06527) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site. | Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:05:36 | 0 | |
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pTrc99S-ssDsbA-TTc-4xDQNAT Resource Report Resource Website |
RRID:Addgene_128401 | Tetanus toxin fragment C domain | C. tetani | Spectinomycin and Streptomycin | PMID:33536221 | Backbone Size:4535; Vector Backbone:pTrc99S; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin | Addgene | 2026-10-24 02:05:37 | 0 |
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